High Throughput PET/CT Imaging Using a Multiple Mouse Imaging System

Abstract

A considerable limitation of current small animal positron emission tomography/computed tomography (PET/CT) imaging is the low throughput of image acquisitions. Subsequently, to design sufficiently-powered studies, high costs accumulate. Together with Mediso Medical Imaging Systems, a four-bed mouse 'hotel' was developed to simultaneously image up to four mice, thereby reducing the cost and maximising radiotracer usage when compared to scans performed with a single mouse bed. Methods. For physiological evaluation of the four-bed mouse hotel, temperature and anaesthesia were tested for uniformity, followed by [ 18 F]fluorodeoxyglucose (FDG) PET/CT imaging of 'mini' image quality (IQ) phantoms specifically designed to fit the new imaging system. Post-reconstruction, National Electrical Manufacturers Association (NEMA) NU-4 tests examined uniformity, recovery coefficients (RCs) and spill-over ratios (SORs). To evaluate the bed under standard in vivo imaging conditions, four mice were simultaneously scanned by dynamic [ 18 F]FDG PET/CT over 60 minutes using the four-bed mouse hotel, with quantified images compared to those acquired using a single mouse bed. Results. The bed maintained a constant temperature of 36.8°C ± 0.4°C (n = 4),with anaesthesia distributed evenly to each nose cone (2.9 ± 0.1 L/min, n = 4). The NEMA tests performed on reconstructed mini IQ phantom images acquired using the four-bed mouse hotel revealed values within the tolerable limits for uniformity, RC values in >2mm rods, and SORs in the non-radioactive water-and air-filled chambers. There was low variability in radiotracer uptake in all major organs of mice scanned using the four-animal bed versus those imaged using a single bed imaging platform. Conclusion. Analysis of images acquired using the fourbed mouse hotel confirmed its utility to increase the throughput of small animal PET imaging without considerable loss of image quality and quantitative precision. In comparison to a single mouse bed, the cost and time associated with each scan were substantially reduced.

Robust and Facile Automated Radiosynthesis of [18F]FSPG on the GE FASTlab

“…Although standard elution in large volumes of eluent may be sufficient for clinical studies using higher starting activities, the The number of mice that can be scanned for a standard 1h dynamic acquisition. Parentheses refer to the number of mice that can be scanned for a standard 1 h acquisition using a 4-bed mouse 'hotel' developed in our lab [31] c…”

“…Dynamic PET scans were acquired on a four-bed mouse hotel [31] for 60 min following a bolus intravenous injection of~3 MBq of radiotracer through a tail vein cannula. CT images were acquired for anatomical visualization (480 projections; helical acquisition; 55 kVp; 600 ms exposure time).…”

Robust and Facile Automated Radiosynthesis of [18F]FSPG on the GE FASTlab

“…b The number of mice that can be scanned for a standard 1h dynamic acquisition. Parentheses refer to the number of mice that can be scanned for a standard 1h acquisition using a 4-bed mouse 'hotel' developed in our lab [32]. c Concentrations obtained using conditions reported in download file view on ChemRxiv Robust and facile automated radiosynthesis of [18F]FSPG ... (2.27 MiB)…”

“…For tumor imaging studies, mice were maintained under anesthesia with isoflurane (1.5-2% in oxygen) at 37 °C during tail vein cannulation and throughout imaging. Dynamic PET scans were acquired on a four bed mouse hotel [32] for 60 min following a bolus intravenous injection of ~3…”

Correlates of Sleep and Arousal via Matrix Methods

“…the time variable), and M denotes the number of recording units whether it be voxels or metabolic concentration levels. In fact, it is exciting that high-throughput PET/CT imaging architectures such as [23] are providing advancement to the acquirement of in vivo metabolic data by providing brain images over an hour recording interval. Additionally, works such as [24] have considered microdialysis and EEG recordings collected simultaneously from the medial prefrontal cortex (mPFC) and primary motor cortex (M1) in mice.…”