High-Throughput Profiling of Metabolic Phenotypes Using High-Resolution GC-MS

Wase, Nishikant; Abshire, Nathan; Obata, Toshihiro

doi:10.1007/978-1-0716-2537-8_19

Cited by 7 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metabolites were extracted with 730 µL of methanol by mixing at 70 °C for 30 min. Following the addition of 750 µL of water and 325 µL of chloroform for phase separation, 50 µL of upper aqueous was dried and derivatized by methoxyamination (37 °C 2 h with 40 µL of 20 mg mL −1 methoxyamine hydrochloride in pyridine) and trimethylsilylation (37 °C for 30 min with 70 µL of N-methyl-N-(trimethylsilyl)trifluoroacetamide) 140,141 . A 1 µL sample of the derivatized material was analyzed in splitless mode using a 7200 GC-QTOF system (Agilent Technologies, Santa Clara, CA, USA).…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms) Metabolite Prof...mentioning

confidence: 99%

Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass

et al. 2022

Self Cite

View full text Add to dashboard Cite

A number of crop wild relatives can tolerate extreme stress to a degree outside the range observed in their domesticated relatives. However, it is unclear whether or how the molecular mechanisms employed by these species can be translated to domesticated crops. Paspalum (Paspalum vaginatum) is a self-incompatible and multiply stress-tolerant wild relative of maize and sorghum. Here, we describe the sequencing and pseudomolecule level assembly of a vegetatively propagated accession of P. vaginatum. Phylogenetic analysis based on 6,151 single-copy syntenic orthologues conserved in 6 related grass species places paspalum as an outgroup of the maize-sorghum clade. In parallel metabolic experiments, paspalum, but neither maize nor sorghum, exhibits a significant increase in trehalose when grown under nutrient-deficit conditions. Inducing trehalose accumulation in maize, imitating the metabolic phenotype of paspalum, results in autophagy dependent increases in biomass accumulation.

show abstract

Section: Gas Chromatography-mass Spectrometry (Gc-ms) Metabolite Prof...mentioning

confidence: 99%

Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The metabolites were further trimethylsylilated by incubating for 30 min at 37 °C with shaking after the addition of 70 μL N-Methyl-N-(trimethylsilyl) trifluoroacetamide (Millipore Sigma). Gas chromatography-mass spectrometry (GC-MS) data acquisition, peak annotation, and quantification were conducted 87 , and the peak heights of the representative ions for each metabolite peak were normalized by that of internal standard (ribitol) to represent relative levels of metabolites. The parameters used for the peak annotation are provided in Supplementary Data 1 .…”

Section: Methodsmentioning

confidence: 99%

Recurrent evolutionary switches of mitochondrial cytochrome c maturation systems in Archaeplastida

Li,

Akella,

Engstler

et al. 2024

Nat Commun

Self Cite

View full text Add to dashboard Cite

Mitochondrial cytochrome c maturation (CCM) requires heme attachment via distinct pathways termed systems I and III. The mosaic distribution of these systems in Archaeplastida raises questions about the genetic mechanisms and evolutionary forces promoting repeated evolution. Here, we show a recurrent shift from ancestral system I to the eukaryotic-specific holocytochrome c synthase (HCCS) of system III in 11 archaeplastid lineages. Archaeplastid HCCS is sufficient to rescue mutants of yeast system III and Arabidopsis system I. Algal HCCS mutants exhibit impaired growth and respiration, and altered biochemical and metabolic profiles, likely resulting from deficient CCM and reduced cytochrome c-dependent respiratory activity. Our findings demonstrate that archaeplastid HCCS homologs function as system III components in the absence of system I. These results elucidate the evolutionary trajectory and functional divergence of CCM pathways in Archaeplastida, providing insight into the causes, mechanisms, and consequences of repeated cooption of an entire biological pathway.

show abstract

“…We used electron ionization with an EI energy of 70 eV, an ion source temperature of 280 °C, an acquired mass range of 70 to 600 amu, and a 50 Hz scan rate. Chromatogram evaluation was conducted according to [ 50 ]. Peaks were annotated using Agilent Masshunter Unknown Analysis software with the Fiehn GC/MS Metabolomics RTL Library (G1676AA; Agilent) as a reference library to produce the batch-specific metabolic library files for use in peak identification and quantification.…”

Section: Methodsmentioning

confidence: 99%

Tissue- and time-dependent metabolite profiles during early grain development under normal and high night-time temperature conditions

Abshire,

Hauck,

Walia

et al. 2024

BMC Plant Biol

Self Cite

View full text Add to dashboard Cite

Background Wheat grain development in the first few days after pollination determines the number of endosperm cells that influence grain yield potential and is susceptible to various environmental conditions, including high night temperatures (HNTs). Flag leaves and seed-associated bracts (glumes, awn, palea, and lemma) provide nutrients to the developing seed. However, the specific metabolic roles of these tissues are uncertain, especially their dynamics at different developmental stages and the time in a day. Tissue- and time-dependent metabolite profiling may hint at the metabolic roles of tissues and the mechanisms of how HNTs affect daytime metabolic status in early grain development. Results The metabolite profiles of flag leaf, bract, seed (embryo and endosperm), and entire spike were analyzed at 12:00 (day) and 23:00 (night) on 2, 4, and 6 days after fertilization under control and HNT conditions. The metabolite levels in flag leaves and bracts showed day/night oscillations, while their behaviors were distinct between the tissues. Some metabolites, such as sucrose, cellobiose, and succinic acid, showed contrasting oscillations in the two photosynthetic tissues. In contrast, seed metabolite levels differed due to the days after fertilization rather than the time in a day. The seed metabolite profile altered earlier in the HNT than in the control condition, likely associated with accelerated grain development caused by HNT. HNT also disrupted the day/night oscillation of sugar accumulation in flag leaves and bracts. Conclusions These results highlight distinct metabolic roles of flag leaves and bracts during wheat early seed development. The seed metabolite levels are related to the developmental stages. The early metabolic events in the seeds and the disruption of the day/night metabolic cycle in photosynthetic tissues may partly explain the adverse effects of HNT on grain yield.

show abstract

High-Throughput Profiling of Metabolic Phenotypes Using High-Resolution GC-MS

Cited by 7 publications

References 30 publications

Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass

Genome of Paspalum vaginatum and the role of trehalose mediated autophagy in increasing maize biomass

Recurrent evolutionary switches of mitochondrial cytochrome c maturation systems in Archaeplastida

Tissue- and time-dependent metabolite profiles during early grain development under normal and high night-time temperature conditions

Contact Info

Product

Resources

About