High-throughput profiling of RNA modifications by ultra-performance liquid chromatography coupled to complementary mass spectrometry: Methods, quality control, and applications

Huang, Gefei; Zhang, Feng; Xie, Dongying; Ma, Yiming; Wang, Pengxi; Cao, Guodong; Chen, Leijian; Lin, Siyi; Zhao, Zhongying; Cai, Zongwei

doi:10.1016/j.talanta.2023.124697

Cited by 3 publications

(1 citation statement)

References 53 publications

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“…Furthermore, the high sensitivity of MS makes it prone to contamination, potentially leading to false positives. 35 This risk underscores the need for a controlled laboratory environment and highly skilled technicians for accurate MS analysis. 30 Additionally, there is an urgent need for advancements in MS process automation, which would reduce the dependence on specialized skills and improve both consistency and efficiency in MS operations.…”

Section: Challengesmentioning

confidence: 99%

Mass spectrometry in clinical laboratory: applications and future prospects

Ghonaim,

Bakr,

Alturki

et al. 2023

Int J Community Med Public Health

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Innovations in laboratory medicine face additional challenges due to the unmet need for extremely precise methods of disease diagnosis. Developments in mass spectrometry-based disease biomarker identification are constantly expanding the field of clinical diagnosis. More mass spectrometry-based in vitro diagnostics are anticipated to move from the bench to the bedside shortly, although several are currently incorporated into standard clinical procedures. The molecular detection by mass spectrometry technique is very effective in diagnosis of the disease and therapeutic monitoring because of its extremely high sensitivity, specificity, and short turnaround time. Mass spectrometry is a potent analytical instrument that may be used to analyse a variety of materials and matrices; its use in clinical laboratory medicine applications is rising. For clinical prognostics and diagnostic purposes, mass spectrometry imaging has been widely utilized to distinguish between healthy and diseased tissues. Modern single-cell studies will benefit from its cutting-edge applications, which will offer comprehensive cellular biochemical data for mechanistic comprehension and, eventually, the development of therapeutic interventions. Within the field of laboratory medicine, the application of mass spectrometry is crucial, especially in terms of diagnostics and therapeutic drug monitoring. This review on the clinical application of mass spectrometry and its future prospects promises an insightful exploration of the evolving landscape of mass spectrometry in clinical settings, delving into its current applications and shedding light on its potential future advancements, for which a comprehensive search in the PubMed, ScienceDirect, and Web of Science databases was conducted.

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Section: Challengesmentioning

confidence: 99%

Mass spectrometry in clinical laboratory: applications and future prospects

Ghonaim,

Bakr,

Alturki

et al. 2023

Int J Community Med Public Health

View full text Add to dashboard Cite

show abstract

A singular chromatographic column breakthrough: Achieving full polarity range separations with the epoxy propanol molecular cage bonded silica stationary phase

Wang,

Li,

Wang

et al. 2024

Journal of Chromatography A

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Epigenetics and plant hormone dynamics: a functional and methodological perspective

Rudolf,

Tomovicova,

Panzarova

et al. 2024

Journal of Experimental Botany

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Plant hormones, pivotal regulators of plant growth, development, and response to environmental cues, have recently emerged as central modulators of epigenetic processes governing gene expression and phenotypic plasticity. This review addresses the complex interplay between plant hormones and epigenetic mechanisms, highlighting the diverse methodologies that have been harnessed to decipher these intricate relationships. We present a comprehensive overview to understand how phytohormones orchestrate epigenetic modifications, shaping plant adaptation and survival strategies. Conversely, we explore how epigenetic regulators ensure hormonal balance and regulate the signalling pathways of key plant hormones. Furthermore, our investigation includes a search for novel genes that are regulated by plant hormones under the control of epigenetic processes. Our review offers a contemporary overview of the epigenetic-plant hormone crosstalk, emphasizing its significance in plant growth, development, and potential agronomical applications.

show abstract

High-throughput profiling of RNA modifications by ultra-performance liquid chromatography coupled to complementary mass spectrometry: Methods, quality control, and applications

Cited by 3 publications

References 53 publications

Mass spectrometry in clinical laboratory: applications and future prospects

Mass spectrometry in clinical laboratory: applications and future prospects

A singular chromatographic column breakthrough: Achieving full polarity range separations with the epoxy propanol molecular cage bonded silica stationary phase

Epigenetics and plant hormone dynamics: a functional and methodological perspective

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