High-throughput proteomic characterization of seminal plasma from bulls with contrasting semen quality

Elango, K.; Karuthadurai, Thirumalaisamy; Kumaresan, A.; Sinha, Manish Kumar; King, John Peter Ebenezer Samuel; Nag, Pranab Kumar; Sharma, Ankur; Raval, Kathan; Paul, Nilendu

doi:10.1007/s13205-023-03474-6

Cited by 7 publications

(2 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, numerous bulls that appear phenotypically healthy end up producing unsatisfactory ejaculates [20] , resulting in the rejection of the ejaculates for AI. These issues could potentially arise from molecular defects within the DNA, RNA, and proteins of the spermatozoa (Prakash et al, 2021;Talluri et al, 2022;Elango et al, 2023;Kumaresan et al, 2023) [20,23,4,11] . The existing method for selecting bulls does not provide a comprehensive evaluation of semen quality since it primarily concentrates on physical characteristics and neglects genetic traits.…”

Section: Introductionmentioning

confidence: 99%

DMXL1 could be a potential candidate gene for semen quality in Holstein Friesian bulls

King,

Kumaresan,

Sinha

et al. 2023

Pharma Innovation

View full text Add to dashboard Cite

The herd's overall financial success is closely tied to the reproductive efficiency of the breeding bulls. Reduced reproductive efficiency in dairy cattle often arises due to the subfertility or infertility of the breeding bulls. In this study, our main focus is on identifying candidate genes containing SNPs within the QTL region, with the goal of using them as markers to predict semen quality. Our study reports the relationship between DMXL1 gene and sperm motility in cattle bulls. Diverse studies emphasized the significance of DMXL1 in various cellular processes and pathways, encompassing cell cycle regulation, cell proliferation, and signal transduction. Further investigation into the DMXL1 gene could offer valuable insights and potentially position it as a candidate gene for semen quality in breeding bulls.

show abstract

Section: Introductionmentioning

confidence: 99%

DMXL1 could be a potential candidate gene for semen quality in Holstein Friesian bulls

King,

Kumaresan,

Sinha

et al. 2023

Pharma Innovation

View full text Add to dashboard Cite

show abstract

“…Despite the rigor of BSE, certain conditions related to the properties of the seminal microenvironment can compromise sperm quality and, consequently, reduce the reproductive capacity of bulls [9]. These conditions are not detected by conventional sperm analysis.…”

Section: Introductionmentioning

confidence: 99%

Relative Abundance of Spermadhesin-1 in the Seminal Plasma of Young Nellore Bulls Is in Agreement with Reproductive Parameters

Ramírez-López,

Barros,

Vidigal

et al. 2023

Veterinary Sciences

View full text Add to dashboard Cite

This study aimed to evaluate the proteomic profile of seminal plasma from young Nellore bulls. We used 20 bulls aged between 19.8 and 22.7 months, divided into two groups according to the results of the Breeding Soundness Evaluation (BSE): approved (FIT n = 10) and not approved (UNFIT n = 10). The scrotal perimeter was measured and a semen collection was performed through electroejaculation. The percentage of sperm motility, mass motility, and sperm vigor were calculated using conventional microscopy, and the percentage of sperm abnormalities was calculated using phase-contrast microscopy of all ejaculates. Seminal plasma was separated from spermatozoa using centrifugation and processed for proteomic analysis by LC-MS/MS. Seminal plasma proteins were identified using MASCOT Daemon software v.2.4.0 and label-free quantification analysis was carried out by SCAFFOLD Q+ software v.4.0 using the Exponentially Modified Protein Abundance Index (emPAI) method. Functional classification of proteins was performed based on their genetic ontology terms using KOG. Functional cluster analysis was performed on DAVID. There were no differences in scrotal perimeter and physical semen characteristics between FIT and UNFIT groups of bulls. The percentage of sperm abnormalities was higher (p < 0.05) in the UNFIT group of bulls. A total of 297 proteins were identified for the two groups. There were a total of 11 differentially abundant proteins (p < 0.05), two of them more abundant in FIT bulls (Spermadhesin-1 and Ig gamma-1 chain C region) and nine in UNFIT bulls (Vasoactive intestinal peptide, Metalloproteinase inhibitor 2, Ig lambda-1 chain C regions, Protein FAM3C, Hemoglobin beta, Seminal ribonuclease, Spermadhesin 2, Seminal plasma protein BSP-30kDa, and Spermadhesin Z13). Spermadhesin-1 was the protein with the highest relative abundance (36.7%) in the seminal plasma among all bulls, corresponding to 47.7% for the FIT bulls and 25,7% for the UNFIT bulls. Posttranslational modification, protein turnover, and chaperones were the functional categories with the highest number of classified proteins. Protein functional annotation clusters were related to Phospholipid efflux, ATP binding, and chaperonin-containing T-complex. The differentially abundant proteins in the group of FIT bulls were related to sperm capacitation and protection against reactive species of oxygen. In contrast, differentially expressed proteins in the group of UNFIT bulls were related to motility inhibition, intramembrane cholesterol removal and oxidative stress. In conclusion, the proteomic profile of the seminal plasma of FIT bulls presents proteins with participation in several biological processes favorable to fertilization, while the proteins of the seminal plasma of UNFIT bulls indicate a series of alterations that can compromise the fertilizing capacity of the spermatozoa. In addition, the relative abundance of spermadhesin-1 found in the seminal plasma of young Nellore bulls could be studied as a reproductive parameter for selection.

show abstract

Transcriptomic and multi-cytokines profile analysis revealed new insights into the integrating mechanisms of cyanidin-3-O-glucoside on male reproductive damage amelioration

Fan,

Xiao,

Zhang

et al. 2024

Food Research International

View full text Add to dashboard Cite

High-throughput proteomic characterization of seminal plasma from bulls with contrasting semen quality

Cited by 7 publications

References 55 publications

DMXL1 could be a potential candidate gene for semen quality in Holstein Friesian bulls

DMXL1 could be a potential candidate gene for semen quality in Holstein Friesian bulls

Relative Abundance of Spermadhesin-1 in the Seminal Plasma of Young Nellore Bulls Is in Agreement with Reproductive Parameters

Transcriptomic and multi-cytokines profile analysis revealed new insights into the integrating mechanisms of cyanidin-3-O-glucoside on male reproductive damage amelioration

Contact Info

Product

Resources

About