High-Throughput Real-Time Analysis of Cell Oxygenation Using Intracellular Oxygen-Sensitive Probes

Abstract

Knowledge of in situ oxygenation of cells in 2D and 3D cultures offers important insights into the impact of oxygen on cellular function. Here we outline how such measurements can be performed in 2D cultures of adherent cells and also in cells cultured on 3D scaffolds. Measurements are performed on conventional time-resolved fluorescence plate readers using the intracellular oxygen-sensitive probe MitoXpress(®)-Intra. We also illustrate how the impact of drug treatment on cell oxygenation can be assessed and how the link between oxygenation and glycolytic metabolism can be examined.

Mitochondrial Dysfunction and Neurodegeneration in Lysosomal Storage Disorders

“…or MitoXpress (Luxcel) systems [53] or a more classical platinum based Clark electrode (e.g. the Oxygraph 2K, Oroboros) instruments, either using purified mitochondria or intact cells.…”

“…In yeasts [25], in fibroblasts [26,27], in hESCs-derived neurons [28], in brains and neurons from a mice model [29] In yeasts [25], in fibroblasts [26,27], in brains and neurons from a mice model [29] In fibroblasts [27] and in lymphoblasts [60] In yeasts [25], in fibroblasts [26], in cellular model [53], in mice liver and cerebellum [56], in murine brain [58] and in cerebellum from patient [59] Lewy bodies in brains from patients [84]; aS oligomers in patients' plasma [86] and in lymphoblasts [87]; cholesterol increased in fibroblasts and in a KO mice brain [29] Krabbe disease…”

Quality and exploitation of umbilical cord blood for cell therapy: Are we beyond our capabilities?

“…Alternatively, an increased oxygen consumption rate by UCB units would also be associated with a high metabolic state (Tejera et al, ). To that end, a variety of highly selective and sensitive probes or reactants and real‐time detection equipment are now available (Zou et al, ; Zaman et al, ; Zhdanov et al, ; Hynes and Carey, ).…”