High-throughput root phenotyping screens identify genetic loci associated with root architectural traits in Brassica napus under contrasting phosphate availabilities

Shi, Lei; Shi, Taoxiong; Broadley, Martin R.; White, Philip J.; Long, Yan; Meng, Jinling; Xu, Fangsen; Hammond, John P.

doi:10.1093/aob/mcs245

Cited by 95 publications

(104 citation statements)

References 69 publications

(105 reference statements)

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“…Several studies have addressed the genetic control of root system vigor in B. napus in the context of adaptation to various environmental conditions (Rahman and McClean 2013), especially drought stress (Fletcher et al 2014) and phosphorous stress (Yang et al 2010;Shi et al 2012). No potential pleiotropic relationships were found between flowering time and root vigor, root biomass, and root length, suggesting common genetic control (Rahman and McClean 2013;Fletcher et al 2014).…”

Section: Genetic Control Of Traits Related To Nitrogen Uptake Efficiencymentioning

confidence: 99%

“…In reciprocal crosses between spring-type and winter-type B. napus, Rahman and McClean (2013) hypothesized a trigenic dominant control of root vigor, based on the segregation ratio of F2 populations. Using linkage analysis on a population of recombinant inbred lines and a doubled haploid population, Yang et al (2010) and Shi et al (2012) detected QTL clusters for root length, lateral root number, root surface area, root biomass, and root volume that were specific to low-phosphorous treatments. Such studies provide the first insight into potentially interesting variation and its underlying genetic control, although care must be taken not to overestimate the importance of major QTLs in mapping populations from strongly differentiated parents, since such QTLs are often dependent on the specific genetic background, particularly in crosses between spring and winter forms.…”

Section: Genetic Control Of Traits Related To Nitrogen Uptake Efficiencymentioning

confidence: 99%

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Nitrogen use efficiency in rapeseed. A review

Bouchet

Laperche

Bissuel-Bélaygue

et al. 2016

Agron. Sustain. Dev.

179

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Mineral nitrogen fertilization has improved crop yield over the last century but has also caused air and water pollution. Reduction of nitrogen inputs and maintaining high yields are therefore essential to ensure a more sustainable agriculture. Improving the nitrogen use efficiency (NUE) of crops is therefore needed. Rapeseed, Brassica napus, depends on nitrogen fertilization due to its low NUE, with the ratio of plant nitrogen content to nitrogen supplied often not exceeding 60 %. Here, we review the major phenotypic traits associated with NUE in B. napus, with special emphasis on winter oilseed rape. We discuss the genetic diversity available and potential breeding strategies. The major points are the following: (1) rapeseed seed yield elaboration is complex, with overlapping phases of nitrogen uptake and remobilization during the crop cycle; (2) traits related to nitrogen uptake, such as root length and the amount of nitrogen absorbed after flowering, and traits related to nitrogen remobilization, such as the "staygreen" phenotype, have been identified as possible levers to improve NUE in rapeseed; (3) a substantial body of studies investigating the genetic control of NUE traits have already published and potential candidate genes identified; and (4) rapeseed genetic diversity may be enriched by exploiting interpopulation genetic variation and the closely related gene pools of Brassica rapa and Brassica oleracea.

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Section: Genetic Control Of Traits Related To Nitrogen Uptake Efficiencymentioning

confidence: 99%

Section: Genetic Control Of Traits Related To Nitrogen Uptake Efficiencymentioning

confidence: 99%

Nitrogen use efficiency in rapeseed. A review

Bouchet

Laperche

Bissuel-Bélaygue

et al. 2016

Agron. Sustain. Dev.

179

View full text Add to dashboard Cite

show abstract

“…PUE-related parameters to be assessed involve lateral root density (primordia and roots/primary root or branching zone length), preferably over a period of time encompassing several stages of seedling development. Shi et al (2013) performed high-throughput assessments of primary and lateral root lengths, and lateral root density using high-throughput imaging of Brassica napus seedling root systems in flat agar trays and ImageJ software (Abràmoff et al 2004). Wang et al (2004) zoomed in on root hair traits, using WinRhizo image analysis software (Regent Instruments Inc., Canada) on images of 1 mm lateral root parts captured by a digital camera on a stereomicroscope.…”

Section: Hydroponics: Growing Plants On Water Solutionsmentioning

confidence: 99%

Improving phosphorus use efficiency in agriculture: opportunities for breeding

Wiel¹,

Linden²,

Scholten³

2015

Euphytica

195

115

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Phosphorus (P) is often an important limiting factor for crop yields, but rock phosphate as fertilizer is a non-renewable resource and expected to become scarce in the future. High P input levels in agriculture have led to environmental problems. One of the ways to tackle these issues simultaneously is improving phosphorus use efficiency (PUE) of the crops through breeding. In this review, we describe plant architectural and physiological traits important for PUE. Subsequently, we discuss efficient methods of screening for PUE traits. We address targeted cultivation methods, including solid and hydroponic systems, as well as testing methods, such as image analysis systems, and biomass and photosynthesis measurements. Genetic variation for PUE traits has been assessed in many crops, and genetics of PUE has been studied by quantitative trait loci (QTL) analyses and genome-wide association study. A number of genes involved in the plant's response to low P have been characterized. These genes include transcription factors, and genes involved in signal transduction, hormonal pathways, sugar signalling, P saving metabolic pathways, and in P scavenging, including transporters and metabolites and/or ATP-ases mobilizing P in the soil. In addition, the role of microorganisms promoting PUE of plants, particularly arbuscular mycorrhizal fungi is discussed. An overview is given of methods for selecting for optimal combinations of plant and fungal genotypes, and their genetics, incl. QTLs and genes involved. In conclusion, significant progress has been made in selecting for traits for PUE, developing systems for the difficult but highly relevant root phenotyping, and in identifying QTLs and genes involved.

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“…Using two contrasting strains to generate a hybrid population and subsequent QTL mapping, a single QTL could be found to explain 20% of the variation between the two parental accessions. Analysis of root traits of a mapping population of Brassica napus for response to low versus high phosphorus levels identified multiple genomic regions (Shi et al, 2013).…”

Section: Natural Variation Of Phosphorous Availability In the Rootmentioning

confidence: 99%