High-throughput screening for ionic liquids dissolving (ligno-)cellulose

Zavrel, Michael; Bross, Daniela; Funke, Matthias; Büchs, Jochen; Spieß, Antje C.

doi:10.1016/j.biortech.2008.11.052

Cited by 652 publications

(395 citation statements)

References 70 publications

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“…In all these treatments, the substantial degradation of lignin is accompanied by considerable reduction in fermentable sugar content of the feedstock, resulting in a loss of 20-35% of the mass of lignocellulose (Galbe and Zacchi 2007;Lee et al 2009). In contrast, recent reports give ample evidence that pretreating biomass with ionic liquids can disrupt the interactions between plant cell wall polymers, resulting in significant improvements in enzymatic hydrolysis kinetics while preventing the loss of fermentable sugars and facilitating the fractionation of biomass (Cheng et al 2011;da Costa Lopes et al 2013;Li et al 2010;Li et al 2009;Mora-Pale et al 2011;Singh et al 2009;Zakrzewska et al 2010;Zavrel et al 2009). …”

Section: Introductionmentioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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Chemical and physical pretreatment of biomass is a critical step in the conversion of lignocellulose to biofuels and bioproducts. Ionic liquid (IL) pretreatment has attracted significant attention due to the unique ability of certain ILs to solubilize some or all components of the plant cell wall. However, these ILs not only inhibit enzyme activities, but also the growth and productivity of microorganisms used in downstream hydrolysis and fermentation processes.While pretreated biomass can be washed to remove residual IL and reduce inhibition, extensive washing is costly and not feasible in large-scale processes. IL tolerant microorganisms and microbial communities have been discovered from environmental samples and studies begun to elucidate mechanisms of IL tolerance. The discovery of IL tolerance in environmental microbial communities and individual microbes has lead to the proposal of molecular mechanisms of resistance. In this article, we review recent progress on discovering IL tolerant microorganisms, identifying metabolic pathways and mechanisms of tolerance, and engineering microorganisms for IL tolerance. Research in these areas will yield new approaches to overcome inhibition in lignocellulosic biomass bioconversion processes and increase opportunities for the use of ILs in biomass pretreatment.

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Section: Introductionmentioning

confidence: 99%

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Simmons

Singer

et al. 2016

Appl Microbiol Biotechnol

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“…The IL 1-ethyl-3-methylimidazolium acetate ( Fig. 1), abbreviated as [C2mim][OAc], has been found to be one of the most promising candidates for biomass pretreatment [27,35].…”

Section: Introductionmentioning

confidence: 99%

Recovery of Sugars from Ionic Liquid Biomass Liquor by Solvent Extraction

et al. 2010

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The dissolution of biomass into ionic liquids (ILs) has been shown to be a promising alternative biomass pretreatment technology, facilitating faster breakdown of cellulose through the disruption of lignin and the decrystallization of cellulose. Both biological and chemical catalysis have been employed to enhance the conversion of IL-treated biomass polysaccharides into monomeric sugars. However, biomass-dissolving ILs, sugar monomers, and smaller carbohydrate oligomers are all soluble in water. This reduces the overall sugar content in the recovered solid biomass and complicates the recovery and recycle of the IL. Nearcomplete recovery of the IL and the holocellulose is essential for an IL-based pretreatment technology to be economically feasible. To address this, a solvent extraction technique, based on the chemical affinity of boronates such as phenylboronic acid and naphthalene-2-boronic acid for sugars, was applied to the extraction of glucose, xylose, and cellobiose from aqueous mixtures of 1-ethyl-3-methylimidazolium acetate. It was shown that boronate complexes could extract up to 90% of mono-and disaccharides from aqueous IL solutions, 100% IL systems, and hydrolysates of corn stover containing IL. The use of boronate complexes shows significant potential as a way to recover sugars at several stages in ionic liquid biomass pretreatment processes, delivering a concentrated solution of fermentable sugars, minimizing toxic byproducts, and facilitating ionic liquid cleanup and recycle.

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“…We report that SCF1 tolerates growth in the presence of >0.5 M 1-ethyl-3-methylimidazolium chloride ([C 2 mim]Cl), an effective biomass pretreatment component (27) that is toxic to most bacteria. The cytotoxicity mechanism of many ionic liquids, specifically [C 2 mim]Cl, has not been investigated; likewise, the molecular basis for rare bacterial tolerance to ionic liquids is not understood.…”

mentioning

confidence: 99%

Global transcriptome response to ionic liquid by a tropical rain forest soil bacterium,Enterobacter lignolyticus

Khudyakov

D’haeseleer

Borglin

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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To process plant-based renewable biofuels, pretreatment of plant feedstock with ionic liquids has significant advantages over current methods for deconstruction of lignocellulosic feedstocks. However, ionic liquids are often toxic to the microorganisms used subsequently for biomass saccharification and fermentation. We previously isolated Enterobacter lignolyticus strain SCF1, a lignocellulolytic bacterium from tropical rain forest soil, and report here that it can grow in the presence of 0.5 M 1-ethyl-3-methylimidazolium chloride, a commonly used ionic liquid. We investigated molecular mechanisms of SCF1 ionic liquid tolerance using a combination of phenotypic growth assays, phospholipid fatty acid analysis, and RNA sequencing technologies. Potential modes of resistance to 1-ethyl-3-methylimidazolium chloride include an increase in cyclopropane fatty acids in the cell membrane, scavenging of compatible solutes, up-regulation of osmoprotectant transporters and drug efflux pumps, and down-regulation of membrane porins. These findings represent an important first step in understanding mechanisms of ionic liquid resistance in bacteria and provide a basis for engineering microbial tolerance.osmotic stress | osmolytes | membrane lipids | differential gene expression | whole genome metabolic reconstruction

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High-throughput screening for ionic liquids dissolving (ligno-)cellulose

Cited by 652 publications

References 70 publications

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Ionic liquid-tolerant microorganisms and microbial communities for lignocellulose conversion to bioproducts

Recovery of Sugars from Ionic Liquid Biomass Liquor by Solvent Extraction

Global transcriptome response to ionic liquid by a tropical rain forest soil bacterium,Enterobacter lignolyticus

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