High-Throughput Screening of Encapsulated Islets Using Wide-Field Lens-Free On-Chip Imaging

Zhang, Hongjie; Alexander, Michael P.; Yang, Sam; Bian, Yinxu; Botvinick, Elliot; Lakey, Jonathan R. T.; Ozcan, Aydogan

doi:10.1021/acsphotonics.8b00343

Cited by 9 publications

(3 citation statements)

References 39 publications

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“…Therefore, an effective platform for the quality control of microcapsules before implantation is essential. To replace the low throughput, high variation and labor-intensive process of the manual microscopic inspection of encapsulated islets, Ozcan et al [ 82 ] developed an ultrahigh-content lens-free on-chip imaging system to rapidly screen alginate-encapsulated islets. The system integrates an optical setup with an ultrawide field of view and a custom-designed sample chamber (Fig.…”

Section: Chip For Quality Control Of Islets For Cell Therapymentioning

confidence: 99%

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Pancreatic islet organoids-on-a-chip: how far have we gone?

Yin

Meng²,

Lin

et al. 2022

J Nanobiotechnol

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Diabetes mellitus (DM) is a disease caused by dysfunction or disruption of pancreatic islets. The advent and development of microfluidic organoids-on-a-chip platforms have facilitated reproduce of complex and dynamic environment for tissue or organ development and complex disease processes. For the research and treatment of DM, the platforms have been widely used to investigate the physiology and pathophysiology of islets. In this review, we first highlight how pancreatic islet organoids-on-a-chip have improved the reproducibility of stem cell differentiation and organoid culture. We further discuss the efficiency of microfluidics in the functional evaluation of pancreatic islet organoids, such as single-islet-sensitivity detection, long-term real-time monitoring, and automatic glucose adjustment to provide relevant stimulation. Then, we present the applications of islet-on-a-chip technology in disease modeling, drug screening and cell replacement therapy. Finally, we summarize the development and challenges of islet-on-a-chip and discuss the prospects of future research. Graphical Abstract

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Section: Chip For Quality Control Of Islets For Cell Therapymentioning

confidence: 99%

“…Reproduced with permission from Ref. [ 82 ]. D The magnetic cell sorting system to purify microencapsulated islets for transplantation.…”

Section: Chip For Quality Control Of Islets For Cell Therapymentioning

confidence: 99%

Pancreatic islet organoids-on-a-chip: how far have we gone?

Yin

Meng²,

Lin

et al. 2022

J Nanobiotechnol

View full text Add to dashboard Cite

show abstract

“…Higher resolution images of entire aggregates are not necessary in this specific application, as the presence of and ratios between two fluorescent reporters are the primary output. Automated image analysis can also be used for morphological analysis of islets encapsulated within biomaterials 147 . This approach has been applied to encapsulation strategies 148 and can be further applied to widely adopted immunoisolation strategies employed by several labs seeking to optimize islet health and engraftment upon transplantation, 13,149,150 as well as stem cell differentiation 151 .…”

Section: High Throughput Approaches To Study β‐(Like)‐cell Biology Anmentioning

confidence: 99%

Engineering-inspired approaches to study β-cell function and diabetes

Lewis

Wells

2021

Stem Cells

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Strategies to mitigate the pathologies from diabetes range from simply administering insulin to prescribing complex drug/biologic regimens combined with lifestyle changes. There is a substantial effort to better understand β-cell physiology during diabetes pathogenesis as a means to develop improved therapies. The convergence of multiple fields ranging from developmental biology to microfluidic engineering has led to the development of new experimental systems to better study complex aspects of diabetes and β-cell biology. Here we discuss the available insulin-secreting cell types used in research, ranging from primary human β-cells, to cell lines, to pluripotent stem cell-derived β-like cells. Each of these sources possess inherent strengths and weaknesses pertinent to specific applications, especially in the context of engineered platforms. We then outline how insulin-expressing cells have been used in engineered platforms and how recent advances allow for better mimicry of in vivo conditions. Chief among these conditions are β-cell interactions with other endocrine organs. This facet is beginning to be thoroughly addressed by the organ-on-a-chip community, but holds enormous potential in the development of novel diabetes therapeutics. Furthermore, high throughput strategies focused on studying β-cell biology, improving β-cell differentiation, or proliferation have led to enormous contributions in the field and will no doubt be instrumental in bringing new diabetes therapeutics to the clinic.

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Organ‐On‐A‐Chip Devices: Technology Progress and Challenges

Obeid,

Yammine,

El‐Nakat

et al. 2024

ChemBioChem

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Organ‐On‐a‐Chip (OOC) is a multichannel 3D‐microfluidic cell‐culture system incorporated in a chip that simulates the behavior of an organ. This technology relies on a multidisciplinary science that benefits from and contributes in the progress of many fields including microbiology, microfluidics, biomaterials, and bioengineering. This review article summarizes the progress and achievements of various organ‐on‐chip technologies. It highlights the significant advantages of this technology in terms of reducing animal testing and providing personalized medical responses. In addition, this paper demonstrates how OOC is becoming a promising and powerful tool in pharmaceutical research to combat diseases. It predicts not only the effects of drugs on the target organs but also, using body‐on‐a‐chip systems, it may provide insights into the side effects of the drug delivery on the other organs. Likewise, the models used for the construction of various organ‐on‐a‐chip devices are investigated along with the design and materials of microfluidic devices. For each OOC, the integrated monitoring devices within the chips (e. g., sensors and biosensors) are discussed. We also discuss the evolution of FDA regulations and the potential in the near future for integrating OOCs into protocols that support and reduce the need for and the failure rates in preclinical and clinical studies.

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High-Throughput Screening of Encapsulated Islets Using Wide-Field Lens-Free On-Chip Imaging

Cited by 9 publications

References 39 publications

Pancreatic islet organoids-on-a-chip: how far have we gone?

Pancreatic islet organoids-on-a-chip: how far have we gone?

Engineering-inspired approaches to study β-cell function and diabetes

Organ‐On‐A‐Chip Devices: Technology Progress and Challenges

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