High-Throughput Screening Using a Synchronized Pulsed Self-aspiration Vacuum Electrospray Ionization Miniature Mass Spectrometer

Abstract

With the advantages of rapid analysis, high sensitivity, and multicomponent identification, mass spectrometry (MS) is recognized as an appealing choice for high-throughput screening (HTS) analysis. Aiming at the small size, simple operation, and adequate performance, the development of miniature mass spectrometers has made great progress over the last 2 decades. Besides the essential analytical performance, simple operation and HTS capability are two other crucial features desired in miniature MS instruments. In this paper, an induced selfaspiration vacuum electrospray ionization source (ISA-VESI) was developed and coupled to a miniature ion trap mass spectrometer. A special timing sequence was designed to synchronize all the operation steps in each measurement, including dual-pulse sample injection, multipulse gas injection, MS analysis, and the movement of the homemade HTS platform used as the sampler. Then, the automatic high-throughput analysis of multiple samples can be accomplished with close coordination among the sample delivery, the sample introduction and ionization, and the ion trap operation. The measurement time of each ISA-VESI-MS analysis was about 7 s, with a sample consumption of less than 100 nL.

Miniature mass spectrometers and their potential for clinical point‐of‐care analysis

“…IM-MS, ion mobility-mass spectrometry; LIT, linear ion traps. [Color figure can be viewed at wileyonlinelibrary.com] analysis, this group proposed an induced self-aspiration vacuum electrospray ionization source and coupled it to a miniature ion trap mass spectrometer (Zhu et al, 2022) (Figure 3D). With a special timing sequence to synchronize all the operation steps, this DAPI system accomplished the automatic high-throughput analysis of multiple samples with an analysis speed of 7 s per sample and sample consumption of less than 100 nL.…”

“…(B) Electromembrane extraction and dual‐channel nanoelectrospray ionization coupled with a miniature mass spectrometer. Reprinted with permission from Zhu et al, 2022. (C) Structural diagram of a miniature ion trap mass spectrometer with membrane inlet and in‐trap photoionization source.…”

A liquid chromatography-miniature mass spectrometry (LC-Mini MS) method for quantitative analysis of risperidone and 9-hydroxyrisperidone in plasma

“…To reduce equipment footprints and simplify instrumental operation, miniaturization of mass spectrometers becomes a practical requirement. 17–22 As a response, in recent years, the development of Mini-MS has made great progress. 23–28…”

“…To reduce equipment footprints and simplify instrumental operation, miniaturization of mass spectrometers becomes a practical requirement. [17][18][19][20][21][22] As a response, in recent years, the development of Mini-MS has made great progress. [23][24][25][26][27][28] In particular, by adopting the continuous atmospheric pressure interface (CAPI), the Mini-MS coupled with ambient ionization sources could greatly simplify sample preparation procedures, and make rapid on-site chemical analysis possible.…”

Hydrogen-Assisted Photoionization and Its Use in Promoting Mass Spectrometry Analysis of VOCs

“…This study was mainly carried out with a homemade ion trap mass spectrometer, which has been depicted previously. , The main components are shown in Figure S1, including a vacuum ultraviolet (VUV) lamp (10.6 eV, PKS 106, Heraeus Deutschland GmbH & Co. KG), a linear ion trap (LIT), and an ion detector. The instrument adopts a discontinuous interface and a single vacuum chamber.…”