High-throughput sequencing method to study the dynamic changes of microbial communities in second-generation fermented lettuce

Zhang, Yao; Chen, Zhidi; Wang, Xinyu; Li, Qingwen; Gao, Xueying; Yi, Xiao Su; Ma, Yumeng

doi:10.1088/1755-1315/680/1/012064

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Operational taxonomic units (OTUs) with 97% similarity cut-off [ 33 , 34 ] were clustered using UPARSE version 7.1, and chimeric sequences were identified and removed. The taxonomy of each OTU representative sequence was analyzed by RDP Classifier version 2.2 [ 35 ] against the 16S rRNA database using a confidence threshold of 0.7 [ 36 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Intestinal Microorganisms on Metabolism and Toxicity Mitigation of Zearalenone in Broilers

Jia

Ren

Yang

et al. 2022

Animals

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Zearalenone (ZEN) is an estrogenic mycotoxin, and chickens are relatively insensitive to it. In this study, the effects of intestinal microorganisms on ZEN metabolism and toxicity mitigation in broilers were studied by two experiments. Firstly, in vitro, ZEN was incubated anaerobically with chyme from each part of the chicken intestine to study its intestinal microbial metabolism. Then, in vivo, we explored the effects of intestinal microbiota on ZEN by inhibiting intestinal microorganisms. Broilers were fed a control diet, 2.5 mg/kg ZEN diet, microbial inhibition diet or ‘microbial inhibition +2.5 mg/kg ZEN’ diet. In vitro, the results showed that the rates of ZEN degradation by microorganisms in the duodenum, ileum, caecum, and colon were 56%, 12%, 15%, and 17%, respectively, and the microorganisms could convert ZEN into Zearalenol (ZOL). After microbial inhibition in vivo, the content of ZEN and its metabolites in excreta of broilers increased significantly, and antioxidant damage and liver damage were aggravated. 16S rRNA sequencing results showed that antioxidant indices and the content of ZEN and its metabolites in excreta were significantly correlated with the relative abundance of Streptococcus, Lactococcus and Enterococcus, etc. In conclusion, the intestinal microorganisms of broilers play an important role in ZEN metabolism and ZEN-induced antioxidant and liver injury mitigation, among which the key bacteria include Streptococcus, Lactococcus and Enterococcus, etc.

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Section: Methodsmentioning

confidence: 99%

Effects of Intestinal Microorganisms on Metabolism and Toxicity Mitigation of Zearalenone in Broilers

Jia

Ren

Yang

et al. 2022

Animals

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“…Weissella spp. are present in most fermentation products [ 50 , 51 ]. In the early stage of fermentation, Weisseria can even dominate in numbers.…”

Section: Discussionmentioning

confidence: 99%

Effects of Fly Maggot Protein Replacement of Fish Meal on Growth Performance, Immune Level, Antioxidant Level, and Fecal Flora of Blue Foxes at Weaning Stage

et al. 2022

Animals

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Dietary protein is a key nutritional parameter and warrants special attention in animal husbandry. This study aimed to evaluate the effect of replacing fish meal (F) with fly maggot protein (M) on the growth performance, antioxidant levels, immune indexes, and fecal microflora in weaned blue foxes (Alopex lagopus). Twenty weaned blue foxes were randomly assigned to the control (F diet; 6% of F) or experimental (M diet; F substituted by M) group (10 blue foxes per group). The duration of the trial was 28 days. The results showed that there was no significant difference in average daily gain between group M and group F during the experiment (p = 0.473). Moreover, the diarrhea index was similar between group M and group F during the entire experimental period (p = 0.112). At the end of the experiment, the levels of IL-6 and IgG in group M at 28 d were significantly higher than that in group F (p = 0.004, p = 0.025, respectively), but not IL-1β, IL-2, SIgA, IgM, and TNF-α. The levels of SOD in group M at 28 d were significantly higher than those in group F (p = 0.001), and no difference of MDA and T-AOC was found between group F and M (p = 0.073, p = 0.196, respectively). In both groups, the diversity of fecal microbes first increased and then decreased with the progress of the experimental period. Initially, there were differences in the composition of microbial communities between the two groups. However, this difference was attenuated at later stages of the experimental period. In conclusion, fly maggot protein can replace fish meal as a source of animal protein in feed material for blue foxes during the weaning period.

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High-throughput sequencing method to study the dynamic changes of microbial communities in second-generation fermented lettuce

Cited by 2 publications

References 8 publications

Effects of Intestinal Microorganisms on Metabolism and Toxicity Mitigation of Zearalenone in Broilers

Effects of Intestinal Microorganisms on Metabolism and Toxicity Mitigation of Zearalenone in Broilers

Effects of Fly Maggot Protein Replacement of Fish Meal on Growth Performance, Immune Level, Antioxidant Level, and Fecal Flora of Blue Foxes at Weaning Stage

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