High-Throughput Sequencing of Oral Microbiota in Candida Carriage Sjögren’s Syndrome Patients: A Pilot Cross-Sectional Study

Xing, Haixia; Liu, Hongwei; Pan, Jie

doi:10.3390/jcm12041559

Cited by 8 publications

(5 citation statements)

References 63 publications

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“…One of the possible reasons why caries experience is higher in dry mouth participants than in controls may be changes in the microbiota due to dry mouth conditions. A 2023 study suggested that such changes are specific for patients with Sjögren’s syndrome, while another study mentioned that dysbiosis is seen in all dry mouth patients despite the etiology of their conditions [ 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Oral health status, related behaviours and perceived stress in xerostomia, Sicca and Sjögren’s syndromes patients - a cross-sectional study

Stankeviciene,

Stangvaltaite-Mouhat,

Aleksejuniene

et al. 2024

BMC Oral Health

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Background Mouth dryness increases the risk of some oral health-related conditions. Furthermore, it is unclear if patients with dry mouth engage in appropriate oral health-related behaviours. The study examined oral health, related behaviours, and perceived stress in dry-mouth patients and compared them to matched controls without mouth dryness. Methods Information about 182 dry-mouth patients and 302 age- and sex-matched subjects was retrieved. Three dry mouth groups: xerostomia, Sicca syndrome and Sjögren’s syndrome, were formed based on patient self-reported and objectively assessed symptoms. The World Health Organization’s Oral Health for Adults and Perceived Stress Scale (PSS-10) questionnaires inquired about sociodemographic characteristics, oral health-related behaviours, and self-perceived stress. Clinical oral health assessments included: caries experience measured as total numbers of decayed (DS), missing (MS), filled surfaces (FS), number of remaining teeth, erosive tooth wear and extent of periodontal pocketing. Data were analyzed using bivariate and multivariable tests. Results The dry-mouth participants had higher mean (SD) DMFS scores than their matched controls: xerostomia patients vs. controls: 74.6 (34.4) and 66.3 (35.4), Sicca syndrome patients vs. controls: 88.3 (34.0) and 70.1 (33.9), and Sjögren’s syndrome patients vs. controls: 95.7 (31.5) and 74 (33.2). In comparison to controls, individuals with Sicca and patients with Sjögren’s syndromes had lower mean (SD) number of remaining teeth, 15.9 (10.1) vs. 21.7 (8.4) and 13.8 (10.0) vs. 20.1 (9.2), and a lower mean (SD) extent of periodontal pocketing, 20.7 (28.6) vs. 41.1 (31.0), and 21.2 (24.1) vs. 34.8 (34.2), respectively. Xerostomia, Sicca syndrome and Sjögren’s syndrome patients had higher odds of using fluoridated toothpaste; OR 1.8 (95%CI 1.1–2.9), OR 5.6 (95%CI 1.7–18.3) and OR 6.9 (95%CI 2.2–21.3), respectively. Participants with Sjögren’s syndrome had lower odds of the last dental visit being within the last year; OR 0.2 (95%CI 0.1–0.8). Conclusions Dry-mouth patients had higher caries experience and fewer teeth than comparison groups but a lower extent of periodontal pocketing. Even though more participants with dry mouth used fluoridated toothpastes, their oral health-related behaviours were not optimal.

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Section: Discussionmentioning

confidence: 99%

Oral health status, related behaviours and perceived stress in xerostomia, Sicca and Sjögren’s syndromes patients - a cross-sectional study

Stankeviciene,

Stangvaltaite-Mouhat,

Aleksejuniene

et al. 2024

BMC Oral Health

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“…The high concentrations of the latter species, in turn, could explain the high incidence of caries in pSS (Siddiqui et al, 2016;Singh et al, 2021). In contrast, a recent study found that dental caries and Candida carriage were independent of SS (Xing et al, 2023).…”

Section: Salivary Microbiomementioning

confidence: 97%

Oral microbial biomap in the drought environment: Sjogren's syndrome

Bustos‐Lobato,

Rus,

Saúco

et al. 2023

Molecular Oral Microbiology

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Sjogren's syndrome (SS) is an autoimmune disease that affects primarily the salivary glands, making perturbations in the oral ecosystem and potential factors of salivary flow that influence the onset and development of the disease. The oral cavity contains diverse microorganisms that inhabit various niches such as the oral microbial “biomap.” It does not seem specific enough to establish a characteristic microbiome, given the diversity of clinical manifestations, variable rates of salivary secretion, and influential risk factors in patients with SS. This review discusses the biogeography of the oral microbiome in patients with SS such as saliva, tongue, tooth, mucosa, and gum. The microorganisms that were more abundant in the different oral niches were Gram‐positive species, suggesting a higher survival of cell wall bacteria in this arid oral environment. Reduced salivary flow appears not to be linked to the cause of dysbiosis alone but influences host‐associated risk factors. However, much work remains to be done to establish the role of the microbiome in the etiopathogenesis of autoimmune diseases such as SS. Future studies of the microbiome in autoimmunity will shed light on the role of specific microorganisms that have never been linked before with SS.

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“…The 20 µL PCR amplification system consisted of 4 µL of 5×FastPfu buffer, 2 µL of dNTPs (2.5 mM), 0.8 µL of forward primer (5 µM), 0.8 µL of reverse primer (5 µM), 0.4 µL of FastPfu polymerase, 0.2 µL of BSA, 10 ng of template DNA, and deionized water. PCR amplification was carried out on an ABI GeneAmp ® 9700 (Applied Biosystems, Waltham, MA, USA) with the following conditions: initial denaturation at 95 • C for 3 min, 35 cycles of 95 • C for 30 s, 55 • C for 30 s, 72 • C for 45 s, and a final extension at 72 • C for 10 min [23]. The amplified products were purified using the AxyPrep DNA Gel Extraction Kit (AXYGEN Biosciences, Union City, CA, USA) [24] and then sequenced using the Illumina MiSeq platform by Major Bio (Shanghai, China).…”

Section: Pcr Amplification and Sequencingmentioning

confidence: 99%

Microbial Community Succession and Its Correlation with Quality Characteristics during Gray Sufu Fermentation

Zhao

Yang

et al. 2023

Foods

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Gray sufu, a traditional fermented food derived from soybeans, undergoes a complex fermentation process. This study aimed to investigate the dynamics of the microbial community during sufu fermentation and its relationship with key quality characteristics. Through systematic sampling of sufu at different phases of fermentation, 143 bacterial genera and 84 fungal genera involved in the process were identified. Among these, Chishuiella, Enterococcus, Lactococcus, and Weissella emerged as the predominant bacterial communities. After seven days of ripening fermentation, Trichosporon supplanted Diutina as the predominant fungus, accounting for more than 84% of all fungi. Using redundancy analysis, significant correlations between microbiota and physicochemical properties were uncovered. Chishuiella and Empedobacter displayed positive relationships with pH, soluble protein, and amino nitrogen content. In addition, five biogenic amines were detected, and it was determined that tyramine accounted for more than 75% of the total biogenic amines in the final gray sufu products. Spearman correlation analysis revealed significant positive relationships between Lactococcus, Enterococcus, Tetragenococcus, Halanaerobium, and Trichosporon and the five biogenic amines examined. These findings shed light on the complex interactions between microorganisms and biogenic amines during the fermentation of gray sufu, thereby facilitating the development of microbial regulation strategies for better quality control.

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High-Throughput Sequencing of Oral Microbiota in Candida Carriage Sjögren’s Syndrome Patients: A Pilot Cross-Sectional Study

Cited by 8 publications

References 63 publications

Oral health status, related behaviours and perceived stress in xerostomia, Sicca and Sjögren’s syndromes patients - a cross-sectional study

Oral health status, related behaviours and perceived stress in xerostomia, Sicca and Sjögren’s syndromes patients - a cross-sectional study

Oral microbial biomap in the drought environment: Sjogren's syndrome

Microbial Community Succession and Its Correlation with Quality Characteristics during Gray Sufu Fermentation

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