High-throughput single-cell analysis of nanoparticle-cell interactions

Haddad, Majood; Frickenstein, Alex N.; Wilhelm, Stefan

doi:10.1016/j.trac.2023.117172

Cited by 7 publications

(2 citation statements)

References 192 publications

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“…Machine learning offers the potential for optimizing parameters and models, significantly reducing processing time [ 154 ]. Moreover, contemporary research in the realm of flow cytometry encompasses cell classification, cell isolation, and their synergistic integration, benefiting from the integration of artificial intelligence techniques and microfluidic methodologies [ 155 ].…”

Section: Future Directions and Challengesmentioning

confidence: 99%

Particle Counting Methods Based on Microfluidic Devices

Dang,

Jiang,

et al. 2023

Micromachines

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Particle counting serves as a pivotal constituent in diverse analytical domains, encompassing a broad spectrum of entities, ranging from blood cells and bacteria to viruses, droplets, bubbles, wear debris, and magnetic beads. Recent epochs have witnessed remarkable progressions in microfluidic chip technology, culminating in the proliferation and maturation of microfluidic chip-based particle counting methodologies. This paper undertakes a taxonomical elucidation of microfluidic chip-based particle counters based on the physical parameters they detect. These particle counters are classified into three categories: optical-based counters, electrical-based particle counters, and other counters. Within each category, subcategories are established to consider structural differences. Each type of counter is described not only in terms of its working principle but also the methods employed to enhance sensitivity and throughput. Additionally, an analysis of future trends related to each counter type is provided.

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Section: Future Directions and Challengesmentioning

confidence: 99%

Particle Counting Methods Based on Microfluidic Devices

Dang,

Jiang,

et al. 2023

Micromachines

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“…Targeted drug delivery, mostly promising in cancer treatment, by magnetic nanoplatforms is related to their interactions at the cellular level, i.e., attachment to the cell, the process of entering it, and its further behavior in the cell [12]. The methods currently used for analyzing interactions at the cell level include flow cytometry, different types of microscopy, and mass spectrometry [13][14][15]. However, these methods have significant limitations, and their applicability is related to specific particles and sensitivity, resolution, and availability, related to technical requirements.…”

Section: Introductionmentioning

confidence: 99%

The Effect of a Magnetic Field on the Transport of Functionalized Magnetite Nanoparticles into Yeast Cells

Dobosz,

Gunia,

Kotarska

et al. 2024

Applied Sciences

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Magnetic nanoparticles are of great interest to scientists as potential drug carriers. Therefore, it is essential to analyze the processes these nanoparticles undergo at the cellular level. The present paper demonstrates the effect of a constant and rotating magnetic field on penetration of TEMPOL-functionalized magnetite nanoparticles into yeast cells. The interactions between nanoparticles and yeast cells without and with a magnetic field were studied using electron spin resonance spectroscopy (ESR). The results showed that the ESR method can monitor the effect of a magnetic field on the magnetite nanoparticle penetration rate into the cells.

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Safety Landscape of Therapeutic Nanozymes and Future Research Directions

Tagaras,

Song,

Sahar

et al. 2024

Advanced Science

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Oxidative stress and inflammation are at the root of a multitude of diseases. Treatment of these conditions is often necessary but current standard therapies to fight excessive reactive oxygen species (ROS) and inflammation are often ineffective or complicated by substantial safety concerns. Nanozymes are emerging nanomaterials with intrinsic enzyme‐like properties that hold great promise for effective cancer treatment, bacterial elimination, and anti‐inflammatory/anti‐oxidant therapy. While there is rapid progress in tailoring their catalytic activities as evidenced by the recent integration of single‐atom catalysts (SACs) to create next‐generation nanozymes with superior activity, selectivity, and stability, a better understanding and tuning of their safety profile is imperative for successful clinical translation. This review outlines the current applied safety assessment approaches and provides a comprehensive summary of the safety knowledge of therapeutic nanozymes. Overall, nanozymes so far show good in vitro and in vivo biocompatibility despite considerable differences in their composition and enzymatic activities. However, current safety investigations mostly cover a limited set of basic toxicological endpoints, which do not allow for a thorough and deep assessment. Ultimately, remaining research gaps that should be carefully addressed in future studies are highlighted, to optimize the safety profile of therapeutic nanozymes early in their pre‐clinical development.

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High-throughput single-cell analysis of nanoparticle-cell interactions

Cited by 7 publications

References 192 publications

Particle Counting Methods Based on Microfluidic Devices

Particle Counting Methods Based on Microfluidic Devices

The Effect of a Magnetic Field on the Transport of Functionalized Magnetite Nanoparticles into Yeast Cells

Safety Landscape of Therapeutic Nanozymes and Future Research Directions

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