High‐throughput species identification: from DNA isolation to bioinformatics

Richardson, David E.; VanWye, Jeffrey; Exum, Amy; Cowen, Robert K.; Crawford, Douglas L.

doi:10.1111/j.1471-8286.2006.01620.x

Cited by 72 publications

(55 citation statements)

References 39 publications

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“…Laboratory data collection. Larval billfishes were sorted from plankton samples and identified to species using morphometrics and lower-jaw pigment patterns or by molecular techniques (Richardson et al 2007). These recent developments in istiophorid larval identification allowed for 800 ind.…”

Section: Methodsmentioning

confidence: 99%

Precocious, selective and successful feeding of larval billfishes in the oceanic Straits of Florida

Llopiz¹,

Cowen²

2008

Mar. Ecol. Prog. Ser.

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ABSTRACT:The oligotrophic open ocean of low latitudes is larval fish habitat for a diversity of resident and migratory species. It is hypothesized that these waters, relative to coastal regions, yield reduced predation mortality, but little is known regarding the feeding and feeding environment of these larvae and the extent to which a nutritional tradeoff may exist, whereby lower predation mortality is accompanied by poor feeding conditions. Monthly sampling of larval billfishes (Istiophoridae) across the Straits of Florida over 2 yr allowed for an investigation of the temporal, spatial and ontogenetic variability in the feeding of sailfish Istiophorus platypterus and blue marlin Makaira nigricans. Consumed prey were numerically dominated (90%) by 2 crustaceans: a copepod (Farranula; mainly F. gracilis) and a cladoceran (Evadne; mainly E. tergestina), with relative proportions displaying marked spatial variability. These prey were consumed throughout early larval ontogeny, from first feeding through piscivorous lengths (> 5 mm), until piscivory became exclusive near 12 mm. High daytime feeding incidence (0.94) and rapid digestion (~3.5 h) support generally frequent and successful feeding by billfish larvae. Prey selectivity was illustrated by preference for Evadne over Farranula and a near absence of calanoid copepods from diets despite high environmental concentrations. Gut fullness exhibited a distinct sunset peak and also differed significantly with larval length and year, but not with season or location. A gut evacuation rate was used to estimate a daily ration of 29 to 75% of gut-free body weight, varying greatly with model selection but also with daylight length. Although potentially unique to the Straits of Florida and larval billfishes, these results contradict the general presumption that the subtropical open ocean is nutritionally constraining for larval fish.KEY WORDS: Fish larvae · Larval fish feeding · Istiophoridae · Piscivory · Ontogenetic shift · Daily ration · Istiophorus platypterus · Makaira nigricans 358: 231-244, 2008 ensure the survival of some offspring in an unpredictable and constraining environment. Consequently, investigating larval fish feeding dynamics in lower latitudes at temporal and spatial scales commensurate with those of spawning is essential to understanding the potential role of feeding in survival to later stages. Resale or republication not permitted without written consent of the publisherMar Ecol Prog SerPrevious work on the feeding of larval istiophorid billfishes in the Indian and Pacific Oceans (Gorbunova & Lipskaya 1975, Uotani & Ueyanagi 1997 suffers from either low sample sizes or limited analyses, especially regarding variability with space and time. In the Atlantic Ocean, there is an even greater paucity of data, with only a handful of small studies reporting on a combined total of 45 larvae (Voss 1953, Gehringer 1956, Schekter 1971. The feeding habits of the youngest individuals, including the first-feeding stage, and any variability with time, space and pre...

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Section: Methodsmentioning

confidence: 99%

Precocious, selective and successful feeding of larval billfishes in the oceanic Straits of Florida

Llopiz¹,

Cowen²

2008

Mar. Ecol. Prog. Ser.

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“…As adult genetic sequences become available, greater use of genetic identification techniques can be made, like those being used to identify anguillid leptocephali (e.g. Aoyama et al 1999Aoyama et al , 2003Aoyama et al , 2007 and proposed for fish larvae in general (Richardson et al 2007;Teletchea 2009). For example, DNA barcoding techniques have recently been applied to many fish species in Australia (Ward et al 2005).…”

Section: -3 Zoogeography and Diversity Of Leptocephalimentioning

confidence: 99%

Ecology of Anguilliform Leptocephali: Remarkable Transparent Fish Larvae of the Ocean Surface Layer

Miller¹

2009

Aqua-BioSci. Monogr. (ABSM)

148

249

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“…Instead, the differences may have arisen because species composition within taxa varied among seasons. Repeated seasonal sampling in multiple years as well as identification by molecular techniques (Richardson et al 2007) would be necessary to test this hypothesis.…”

Section: Discussionmentioning

confidence: 99%

Vertical migrations of reef fish larvae in the Straits of Florida and effects on larval transport

Huebert

Cowen

Sponaugle

2011

Limnology & Oceanography

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The goals of this study were to measure vertical distributions of pelagic coral reef fish larvae, identify significant vertical migrations, and estimate the effects of vertical migrations between depths with different ambient currents on larval transport in the Straits of Florida. Spring, summer, and fall time-series of plankton net tows were conducted at the edge of the Florida Current offshore of Miami. In each time-series the upper 100 m of the water column at a fixed station was sampled repeatedly at 3-h intervals for two diel cycles, with different nets sampling at five discrete depth ranges. Simultaneously, currents-at-depth were recorded by shipboard current profilers. Mean and standard deviation of larval depth and statistically significant vertical migrations were determined by nonparametric resampling and tree regression techniques. In 10 taxa, larvae showed ontogenic vertical migrations, gradually moving deeper with growth and development. Vertical distributions of six taxa varied between day and night, revealing diel vertical migrations. Larval transport was estimated by generating progressive vector diagrams from current measurements and larval vertical distributions. The dominant alongshore component of the Florida Current resulted in rapid larval transport through the Straits of Florida, while cross-shore current was an order of magnitude weaker and highly variable. Larvae at depths . 70 m experienced a 15-75% reduction in transport; thus, deep vertical distributions and downward vertical migrations potentially favor retention and settlement in the Straits of Florida.

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High‐throughput species identification: from DNA isolation to bioinformatics

Cited by 72 publications

References 39 publications

Precocious, selective and successful feeding of larval billfishes in the oceanic Straits of Florida

Precocious, selective and successful feeding of larval billfishes in the oceanic Straits of Florida

Ecology of Anguilliform Leptocephali: Remarkable Transparent Fish Larvae of the Ocean Surface Layer

Vertical migrations of reef fish larvae in the Straits of Florida and effects on larval transport

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