High-throughput SPR sensor for food safety

Piliarik, Marek; Párová, Lucie; Homola, Jiřı́

doi:10.1016/j.bios.2008.08.012

Cited by 233 publications

(132 citation statements)

References 32 publications

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“…The p-polarized light was focused by a collimator lens (8) onto a reflection mirror (9). At last, the reflected light by the reflection mirror passed through a diffusion correction lens (10), and finally was projected to the center of the linear CCD sensor on pixel numbers between 461 and 1320 (11), as shown in a 3-D view of the linear CCD sensor in Fig. 1.…”

Section: Optical System and System Integration Of Present Spr Sensormentioning

confidence: 99%

“…[11][12][13][14][15][16][17][18][19][20][21] The first type of SPR sensor is a so-called SPR imaging sensor, where a 2-dimension CCD sensor is used for measuring reflected light from the sensor chip, on which a multi-channel or micro array was fabricated. [11][12][13][14] For example, Piliarik et al 11 reported a high-throughput SPR imaging sensor for nucleic acids, while identifying a specific bacteria pathogenous. The sensitivity resolution of the sensor was 2 × 10 -7 RIU, and the detection of nucleic acid at the 100 pM level was achieved.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor

et al. 2016

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A portable-type surface plasmon resonance (SPR) sensor, composed from a new optical system for multi-sensing, has been developed to apply to environment analysis, clinical diagnosis etc., where many samples are desired to be analyzed at high throughput. The optical system of the sensor consists of a light-emitting diode, a pair of cylindrical lenses, a pair of collimator lenses, a correction lens, a prism, a polarizer and a linear CCD sensor with 2048 pixels. Reflected light from a sensor chip of the width of 6 mm at a certain incident angle was detected by ca. 618 pixels of the linear CCD sensor as an SPR sensor signal. An SPR sensor signal at a specified incident angle is controllable for optimization by adjusting the position of the CCD sensor. A sensor chip having a 30-stripe linear pattern (100 μm width/stripe) was prepared. The spatial resolution as well as the performance of the sensor were evaluated by using sucrose solutions. As a result, the acquisition of SPR sensor signals from 30 sensing points was successfully achieved with a spatial resolution of 100 μm (distance between 2 sensing points). A lower detection limit of ca. 3.2 -5.5 × 10 -5 RIU with a standard deviation of ±4.5% was obtained by averaging the signals from 6 -7 pixels of the CCD sensor per one sensing stripe.

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Section: Optical System and System Integration Of Present Spr Sensormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor

et al. 2016

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“…As can be seen from the example in the Figure 3b inset, 16 S ribosomal RNA from Escherichia coli (E coli) could easily be screened using SPR imaging in conjunction with DNA microarrays fabricated on a self-assembled monolayer of the amineterminated alkanethiol modified gold surface [21]. Homola et al also employed a SPR imaging platform for the detection of foodborn pathogens [22] utilizing an array of sensing channels prepared by microspotting various thiolated DNA probe solutions complementary to specific 16 S ribosomal RNAs sequences of selected pathogen targets. Furthermore, SPR sensors are readily amenable to incorporation into portable and/or sensing platforms for pathogen taking advantage of the developments in microfluidics and the fabrication of miniaturized optics [23][24].…”

Section: Surface Plasmon Resonance (Spr)mentioning

confidence: 99%

Bioaffinity detection of pathogens on surfaces

Wark

Lee

Kim

et al. 2010

Journal of Industrial and Engineering Chemistry

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The demand for improved technologies capable of rapidly detecting pathogens with high sensitivity and selectivity in complex environments continues to be a significant challenge that helps drive the development of new analytical techniques. Surface-based detection platforms are particularly attractive as multiple bioaffinity interactions between different targets and corresponding probe molecules can be monitored simultaneously in a single measurement. Furthermore, the possibilities for developing new signal transduction mechanisms alongside novel signal amplification strategies are much more varied. In this article, we describe some of the latest advances in the use of surface bioaffinity detection of pathogens. Three major sections will be discussed: (i) a brief overview on the choice of probe molecules such as antibodies, proteins and aptamers specific to pathogens and surface attachment chemistries to immobilize those probes onto various substrates, (ii) highlighting examples among the current generation of surface biosensors, and (iii) exploring emerging technologies that are highly promising and likely to form the basis of the next generation of pathogenic sensors.

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“…Since the dependence of the phase responseon refractive index is much stronger than that of the amplitude response, phase-sensitive SPR sensor emerges as a promising resolution to provide lower detection limit in late 1990s [7][8][9]. During the last two decades, phasesensitive SPR sensors received extensive research in order to improve the detection limit [10], dynamic range [11][12][13][14] and detection throughput [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Surface Plasmon Resonance Phase-Sensitive Imaging (Spr-Pi) Sensor Based on a Novel Prism Phase Modulator

Ye¹,

Yang²,

Jiang³

et al. 2014

PIER

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Abstract-A novel prism phase modulator (PPM) for phase difference modulation between p-and s-polarization lights in a surface plasmon resonance phase-sensitive imaging sensor is proposed in this paper. The PPM consists of a rhombic prism (to obtain a curve of phase difference between the two polarizations), a rotation stage and a mirror. The PPM shows great modulation stability and helps to achieve a high detection resolution. Surface plasmon resonance phase imaging is realized with a microfluidic device and a CCD camera. Experimental result shows that the detection resolution of our SPR-PI sensor based on phase-interrogation method is 7.61 × 10 −7 RIU with hydrous samples, which is 16 times improved compared with that based on intensity-interrogation. Real-time monitoring of the interaction between Angiogenin and anti-Angiogenin is also illustrated.

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High-throughput SPR sensor for food safety

Cited by 233 publications

References 32 publications

Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor

Development of a Portable Surface Plasmon Resonance Sensor with Multi-Sensing Points Based on the Linear CCD Sensor

Bioaffinity detection of pathogens on surfaces

Surface Plasmon Resonance Phase-Sensitive Imaging (Spr-Pi) Sensor Based on a Novel Prism Phase Modulator

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