High-throughput synchronous erythrocyte cellular antioxidant activity and protection screening of phenolic-rich extracts: Protocol validation and applications

Cruz, Thiago Mendanha; Lima, Amanda dos Santos; Silva, Alessandra Oliveira; Mohammadi, Nima; Zhang, Liang; Azevedo, Luciana; Marques, Mariza Boscacci; Granato, Daniel

doi:10.1016/j.foodchem.2023.138281

Cited by 7 publications

(2 citation statements)

References 43 publications

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“…For example, various conditions are used in chemical antioxidant assays. These include varying pH levels (ranging from 3 to 12), temperatures (typically between 25 and 37 °C), reaction times (ranging from 5 min to 2 h), and the use of probes and radicals like DPPH, which are not commonly found in biological systems ( Apak et al, 2022 ; Cruz et al, 2024 ). However, biological systems are conducted at physiological pH and temperature, focusing on accounting for metabolism and intracellular transportation.…”

Section: Resultsmentioning

confidence: 99%

Bridging the gap in antioxidant activity of flavonoids: Correlating the oxidation of human plasma with chemical and cellular assays

Mohammadi,

dos Santos Lima,

Azevedo

et al. 2024

Current Research in Food Science

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Section: Resultsmentioning

confidence: 99%

Bridging the gap in antioxidant activity of flavonoids: Correlating the oxidation of human plasma with chemical and cellular assays

Mohammadi,

dos Santos Lima,

Azevedo

et al. 2024

Current Research in Food Science

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“…Platelet activation is associated with increased ROS formation [54,55], and flavonoid aglycones demonstrate antioxidant activity in different cell types [56,57]. Nonetheless, it remains unclear whether their effect is related to the ROS formation decrease in platelets.…”

Section: Flavonoid Aglycones Reduce Ros Formation In Thrombin-activat...mentioning

confidence: 99%

Antiplatelet Effects of Flavonoid Aglycones Are Mediated by Activation of Cyclic Nucleotide-Dependent Protein Kinases

Balykina,

Naida,

Kirkgöz

et al. 2024

IJMS

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Flavonoid aglycones are secondary plant metabolites that exhibit a broad spectrum of pharmacological activities, including anti-inflammatory, antioxidant, anticancer, and antiplatelet effects. However, the precise molecular mechanisms underlying their inhibitory effect on platelet activation remain poorly understood. In this study, we applied flow cytometry to analyze the effects of six flavonoid aglycones (luteolin, myricetin, quercetin, eriodictyol, kaempferol, and apigenin) on platelet activation, phosphatidylserine externalization, formation of reactive oxygen species, and intracellular esterase activity. We found that these compounds significantly inhibit thrombin-induced platelet activation and decrease formation of reactive oxygen species in activated platelets. The tested aglycones did not affect platelet viability, apoptosis induction, or procoagulant platelet formation. Notably, luteolin, myricetin, quercetin, and apigenin increased thrombin-induced thromboxane synthase activity, which was analyzed by a spectrofluorimetric method. Our results obtained from Western blot analysis and liquid chromatography–tandem mass spectrometry demonstrated that the antiplatelet properties of the studied phytochemicals are mediated by activation of cyclic nucleotide-dependent signaling pathways. Specifically, we established by using Förster resonance energy transfer that the molecular mechanisms are, at least partly, associated with the inhibition of phosphodiesterases 2 and/or 5. These findings underscore the therapeutic potential of flavonoid aglycones for clinical application as antiplatelet agents.

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Bioaccessibility of bioactive compounds from Pereskia aculeata and their cellular antioxidant effect

Cruz,

Lima,

Zhou

et al. 2024

Food Chemistry

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High-throughput synchronous erythrocyte cellular antioxidant activity and protection screening of phenolic-rich extracts: Protocol validation and applications

Cited by 7 publications

References 43 publications

Bridging the gap in antioxidant activity of flavonoids: Correlating the oxidation of human plasma with chemical and cellular assays

Bridging the gap in antioxidant activity of flavonoids: Correlating the oxidation of human plasma with chemical and cellular assays

Antiplatelet Effects of Flavonoid Aglycones Are Mediated by Activation of Cyclic Nucleotide-Dependent Protein Kinases

Bioaccessibility of bioactive compounds from Pereskia aculeata and their cellular antioxidant effect

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