High titer heterologous rhamnolipid production

Beuker, Janina; Barth, Theresa; Steier, Anke; Wittgens, Andreas; Rosenau, Frank; Henkel, Marius; Hausmann, Rudolf

doi:10.1186/s13568-016-0298-5

Cited by 51 publications

(60 citation statements)

References 13 publications

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“…In spite of advantages of foam fractionation's utility‐free, high effectiveness, and continuous product removal, high growth and concentration of bacteria in the cultivation media could result in their entrapment into the foam fraction and cause problems in the integration of foam fractionator into the bioreactor . Nonetheless, some approaches has been developed to overcome these obstacles, that including magnetic separation or using immobilized bacterial cells systems may be required to capture or return bacteria in the cultivation medium to separate growth from products recovery .…”

Section: Challenges Of Foam Fractionationmentioning

confidence: 99%

“…Rhamnolipids contain one or two rhamnose sugar molecules and one or two molecules of hydroxy fatty acids with a variety of carbon molecules lengths ranging from 8 to 14. Rhamnolipids are mostly produced by Pseudomonas aeruginosa , can reduce surface tension of water from 72 to 25–30 mN/m and exhibit critical micelle concentration (CMC) of about 10–200 mg/L . On the other hand, the most studied bacteria that produce surfactins are the Bacilli such as Bacillus subtilis .…”

Section: Introductionmentioning

confidence: 99%

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In situ downstream strategies for cost‐effective bio/surfactant recovery

Najmi

Ebrahimipour

Franzetti

et al. 2018

Biotech and App Biochem

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Since 60-80% of total costs of production are usually associated with downstream collection, separation, and purification processes, it has become advantageous to investigate how to replace traditional methods with efficient and cost-effective alternative techniques for recovery and purification of biosurfactants. In the traditional techniques, large volumes of organic solvents are usually used for increasing production cost and the overall environmental burden. In addition, traditional production and separation methods typically carried out in batch cultures reduce biosurfactant yields due to product inhibition and lower biosurfactants activity as a result of interaction with the organic solvents used. However, some in situ recovery methods that allow continuous separation of bioproducts from culture broth leading to an improvement in yield production and fermentation efficiency. For biosurfactants commercialization, enhancement of product capacity of the separation methods and the rate of product removal is critical. Recently, interest in the integration of separation methods with a production step as rapid and efficient techniques has been increasing. This review focuses on the technology gains and potentials for the most common methods used in in situ product removal: foam fractionation and ultrafiltration, especially used to recover and purify two well-known biosurfactants: glycolipids (rhamnolipids) and lipopeptides (surfactins).

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Section: Challenges Of Foam Fractionationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In situ downstream strategies for cost‐effective bio/surfactant recovery

Najmi

Ebrahimipour

Franzetti

et al. 2018

Biotech and App Biochem

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“…This gram‐negative, ubiquitous, saprophytic soil bacterium has become a remarkable workhorse for biotechnical processes (Loeschcke & Thies, ; Martins Dos Santos et al, ). As an example, it is a suitable host for the production of the biosurfactant rhamnolipid (Arnold, Henkel, et al, ; Beuker, Barth, et al, , Beuker, Steier, et al, ; Cha, Lee, Kim, Kim, & Lee, ; Tiso et al, ; Wittgens et al, , , ). Unlike many other Pseudomonads , P. putida KT2440 is classified as biosafety level 1 according to American Type Culture Collection.…”

Section: Introductionmentioning

confidence: 99%

Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio‐based economy

et al. 2019

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Lignocellulose‐derived hydrolyzates typically display a high degree of variation depending on applied biomass source material as well as process conditions. Consequently, this typically results in variable composition such as different sugar concentrations as well as degree and the presence of inhibitors formed during hydrolysis. These key obstacles commonly limit its efficient use as a carbon source for biotechnological conversion. The gram‐negative soil bacterium Pseudomonas putida KT2440 is a promising candidate for a future lignocellulose‐based biotechnology process due to its robustness and versatile metabolism. Recently, P. putida KT2440_xylAB which was able to metabolize the hemicellulose (HC) sugars, xylose and arabinose, was developed and characterized. Building on this, the intent of the study was to evaluate different lignocellulose hydrolyzates as platform substrates for P. putida KT2440 as a model organism for a bio‐based economy. Firstly, hydrolyzates of different origins were evaluated as potential carbon sources by cultivation experiments and determination of cell growth and sugar consumption. Secondly, the content of major toxic substances in cellulose and HC hydrolyzates was determined and their inhibitory effect on bacterial growth was characterized. Thirdly, fed‐batch bioreactor cultivations with hydrolyzate as the carbon source were characterized and a diauxic‐like growth behavior with regard to different sugars was revealed. In this context, a feeding strategy to overcome the diauxic‐like growth behavior preventing accumulation of sugars is proposed and presented. Results obtained in this study represent a first step and proof‐of‐concept toward establishing lignocellulose hydrolyzates as platform substrates for a bio‐based economy.

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“…Rhamnolipids are one of the most intensively studied microbial produced biosurfactants. They lower surface tension of water from 72 to 25-30 mN m −1 and exhibit critical micelle concentration (CMC) as low as 10-200 mg L −1 (Beuker et al, 2016). Production of rhamnolipids is mainly described in the opportunistic pathogen Pseudomonas aeruginosa using shake flask, batch, fed-batch or continuous systems (Beuker et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…They lower surface tension of water from 72 to 25-30 mN m −1 and exhibit critical micelle concentration (CMC) as low as 10-200 mg L −1 (Beuker et al, 2016). Production of rhamnolipids is mainly described in the opportunistic pathogen Pseudomonas aeruginosa using shake flask, batch, fed-batch or continuous systems (Beuker et al, 2016). Researchers have worked with various combinations of carbon and nitrogen sources in biosurfactants production technology (Banat et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies on the Biosurfactant Production Capacity of <i>Bacillus Subtilis</i> and <i>Pseudomonas Aeruginosa</i> Using Engine Oil and Diesel Respectively as Substrate

Abdulsalam¹,

Mohammed²,

Garba³

et al. 2017

Nig J Bas App Sci

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The study was carried out to compare the the production capabilities and the biosurfactant activity of the bacteria, Bacillus subtilis and Pseudomonas aeruginosa using engine oil and diesel as the substrates respectively. The test organisms were isolated from engine oil contaminated soil as in the case of the Bacillus subtilis, which was collected from an automobile workshop in Samaru, Zaria and hydrocarboncontaminated water in the case of the Pseudomonas aeruginosa. The medium used for the experiment was a mineral medium supplemented with 2% engine oil and 2% diesel as the sole source of carbon and energy for Bacillus subtilis and Pseudomonas aeruginosa respectively. Production of biosurfactant was assayed by monitoring the increase in cell concentration, biosurfactant concentration, emulsification index and decrease in surface tension. Highest level of cell concentration and biosurfactant concentration (3.3 x 10 8 CFU/ml and 0.0106mg/ml respectively) were obtained at 144 h for the Pseudomonas aeruginosa using diesel as source of carbon and energy while the highest level of cell concentration and biosurfactant concentration (3.2 x 10 8 CFU/ml and 0.0096mg/ml respectively) were obtained at 120hrs for the Bacillus subtilis using engine oil as source of carbon and energy. The research show that Pseudomonas aeruginosa using diesel as the sole source of carbon and energy is better for the production of biosurfactant than Bacillus subtilis using engine oil as the sole source of carbon and energy.

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High titer heterologous rhamnolipid production

Cited by 51 publications

References 13 publications

In situ downstream strategies for cost‐effective bio/surfactant recovery

In situ downstream strategies for cost‐effective bio/surfactant recovery

Potential of biotechnological conversion of lignocellulose hydrolyzates by Pseudomonas putida KT2440 as a model organism for a bio‐based economy

Comparative Studies on the Biosurfactant Production Capacity of <i>Bacillus Subtilis</i> and <i>Pseudomonas Aeruginosa</i> Using Engine Oil and Diesel Respectively as Substrate

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