High titre Hog Cholera virus production of Cytodex 3® microcarrier cultures

Caij, Ann Brigitte; Smet, A. De; Dubois, Nicolas; Koenen, F.

doi:10.1007/bf01311121

Cited by 5 publications

(3 citation statements)

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“…This may be an ELISA based on monoclonal antibodies directed against other structural or nonstructural viral proteins than the gp 55. In this report, we describe the use of high titer hog choIera virus preparations, produced on Cytodex3 [1] as antigen for the production of monoclonal antibodies. These monoclonal antibodies were characterized both by a neutralization test and by reacting them against different HCV, BVDV strains and HCV isolates from different disease outbreaks of the last decade in Belgium.…”

Section: Production and Characterization Of Monoclonal Antibodies Agamentioning

confidence: 99%

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Production and characterization of monoclonal antibodies against hog cholera virus (Alfort 187 strain)

Caij

Muyldermans

Smet

et al. 1993

Archives of Virology

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Summary.A panel of 15 monoclonal antibodies (Mabs) against hog cholera virus (HCV) was produced and each Mab was characterized according to its viral protein specificity, virus-neutralizing activity and immunoreactivity with a large collection of HCV isolates.Hog cholera (HC) is a contagious and economically important disease of pigs and is caused by hog cholera virus (HCV). HCV belongs to the genus pestivirus of the family Flaviviridae [5]. This genus is composed of hog cholera virus, bovine viral diarrhoea virus (BVDV) of cattle and border disease virus (BDV) of sheep. These viruses show strong structural and serological similarities [2,21].Pestiviruses are small enveloped, infectious positive stranded animal RNA viruses with a diameter of about 40 nm, and possess a nucleocapsid assumed to have an icosahedral symmetry [8]. The purification of virions by physical means is extremely difficult [10] due to the problems inherent to pestivirus research, i.e., low titers in cell cultures, membrane association and fragility of viral particles. Despite these purification difficulties, monoclonal antibodies against the HCV were raised in different laboratories. Most of the Mabs produced against the HCV are directed against the major glycoprotein gp 55 [6,18,19], but Mabs were also obtained which recognized the gp44/48 of the Alfort strain and the gp 42 of the "Chinese" vaccine strain [15,20]. Many of the monoclonal antibodies directed against the major glycoprotein were shown to neutralize virus infectivity. Four antigenic domains on the structural gp 55

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Section: Production and Characterization Of Monoclonal Antibodies Agamentioning

confidence: 99%

“…For the production of monoclonal antibodies, the HCV strain Alfort 187 (EEC-reference strain) was grown on PK-15 cells, adapted to microcarrier cultures [1]. As growth medium, Earle's MEM supplemented with antibiotics and 10% horse serum was used.…”

Section: Production and Characterization Of Monoclonal Antibodies Agamentioning

confidence: 99%

Production and characterization of monoclonal antibodies against hog cholera virus (Alfort 187 strain)

Caij

Muyldermans

Smet

et al. 1993

Archives of Virology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Virus was produced on pig kidney cells (PK-15) grown on Cytodex 3 microcarrier culture as described [2]. The virus titer was increased by ultracentrifugation of the culture fluid giving a final titer of 5 x 10 l° TCIDs0/ml.…”

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confidence: 99%