High tolerance of chemolithoautotrophic sulphur oxidizing bacteria towards pulp and paper mill wastewaters and their organic constituents supporting sulphur recovery in alkaline conditions

Hajdu-Rahkama, Réka; Puhakka, Jaakko A.

doi:10.1016/j.cej.2022.137972

Cited by 7 publications

(2 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, the salinity of the soil/sediment’s is unsuitable for agricultural purposes, as high salt levels can adversely impact crop yields, nutrient availability, and the activity of the microbial community inhabiting the soil. It’s worth noting that several authors have reported elevated levels of these cations and anions in wastewater ( Eskelinen et al, 2010 ; Hajdu-Rahkama and Puhakka, 2022 ) and paper sludge ( Chandra et al, 2017 ; Singh et al, 2020 ). In India, farmers widely use chloride-containing bleach wastewater in irrigation as it not adsorbed by soil and moves quickly travels with soil water, gets adsorbed by crops, and ultimately accumulates in the leaves.…”

Section: Discussionmentioning

confidence: 99%

“…To date, only an inadequate number of microbial agents have been identified that can effectively degrade and treat wastewater or pollutants discharged from PPMs ( Fernandes et al, 2014 ; Sonkar et al, 2019 ; An et al, 2021 ; Hajdu-Rahkama and Puhakka, 2022 ; Yue et al, 2023 ). This scarcity of microbial agents capable of treating paper mill wastewater may be attributed to challenges in isolating and culturing these microbial species under controlled conditions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Unraveling the shift in bacterial communities profile grown in sediments co-contaminated with chlorolignin waste of pulp-paper mill by metagenomics approach

Kumar,

Ameen,

Verma

2024

Front. Microbiol.

View full text Add to dashboard Cite

Pulp-paper mills (PPMs) are known for consistently generating a wide variety of pollutants, that are often unidentified and highly resistant to environmental degradation. The current study aims to investigate the changes in the indigenous bacterial communities profile grown in the sediment co-contaminated with organic and inorganic pollutants discharged from the PPMs. The two sediment samples, designated PPS-1 and PPS-2, were collected from two different sites. Physico-chemical characterization of PPS-1 and PPS-2 revealed the presence of heavy metals (mg kg−1) like Cu (0.009–0.01), Ni (0.005–0.002), Mn (0.078–0.056), Cr (0.015–0.009), Pb (0.008–0.006), Zn (0.225–0.086), Fe (2.124–0.764), Al (3.477–22.277), and Ti (99.792–45.012) along with high content of chlorophenol, and lignin. The comparative analysis of organic pollutants in sediment samples using gas chromatography–mass spectrometry (GC–MS) revealed the presence of major highly refractory compounds, such as stigmasterol, β-sitosterol, hexadecanoic acid, octadecanoic acid; 2,4-di-tert-butylphenol; heptacosane; dimethyl phthalate; hexachlorobenzene; 1-decanol,2-hexyl; furane 2,5-dimethyl, etc in sediment samples which are reported as a potential toxic compounds. Simultaneously, high-throughput sequencing targeting the V3–V4 hypervariable region of the 16S rRNA genes, resulted in the identification of 1,249 and 1,345 operational taxonomic units (OTUs) derived from a total of 115,665 and 119,386 sequences read, in PPS-1 and PPS-2, respectively. Analysis of rarefaction curves indicated a diversity in OTU abundance between PPS-1 (1,249 OTUs) and PPS-2 (1,345 OTUs). Furthermore, taxonomic assignment of metagenomics sequence data showed that Proteobacteria (55.40%; 56.30%), Bacteoidetes (11.30%; 12.20%), and Planctomycetes (5.40%; 4.70%) were the most abundant phyla; Alphproteobacteria (20.50%; 23.50%), Betaproteobacteria (16.00%; 12.30%), and Gammaproteobacteria were the most recorded classes in PPS-1 and PPS-2, respectively. At the genus level, Thiobacillus (7.60%; 4.50%) was the most abundant genera grown in sediment samples. The results indicate significant differences in both the diversity and relative abundance of taxa in the bacterial communities associated with PPS-2 when compared to PPS-1. This study unveils key insights into contaminant characteristics and shifts in bacterial communities within contaminated environments. It highlights the potential for developing efficient bioremediation techniques to restore ecological balance in pulp-paper mill waste-polluted areas, stressing the importance of identifying a significant percentage of unclassified genera and species to explore novel genes.

show abstract