High total antioxidant capacity of the porcine seminal plasma (SP-TAC) relates to sperm survival and fertility

Barranco, Isabel; Tvarijonaviciute, Asta; Pérez-Patiño, Cristina; Parrilla, Inmaculada; Cerón, José J.; Martı́nez, Emilio A.; Rodriguez‐Martinez, Heriberto; Roca, Jordi

doi:10.1038/srep18538

Cited by 71 publications

(58 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this context, it is well known that the total protein composition differs among boar ejaculate fractions [43]. As in human, the boar ejaculate is expelled in fractions, the SRF and the post-SRF being the two main fractions, with relevant differences in SP-composition [43] including cytokine-contents [44] and antioxidant enzymes [45]. Further research is required to verify possible divergences in the SP-proteome among ejaculate fractions/portions, particularly in the light of the ongoing discussions as to whether to use only the SRF or the entire ejaculate for both AI and sperm technologies, such as cryopreservation and sex-sorting [2].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

Pérez-Patiño

Barranco

Parrilla

et al. 2016

Journal of Proteomics

Self Cite

102

View full text Add to dashboard Cite

Full identification of SP-proteins remains challenging, particularly in some livestock species such as porcine. This experimental study aims to provide an extensive proteomic analysis of boar SP and to generate a public accessible database of boar SP-proteome. A SP-pool from 33 entire ejaculates from 11 boars (3 ejaculates per boar) was analyzed to characterize the boar SP-proteome. Moreover, 20 ejaculates collected in fractions (P1: first 10 mL of sperm rich ejaculate fraction (SRF), P2: rest of SRF and P3: post-SRF) from 5 boars (4 ejaculates per boar) were analyzed to evaluate differentially expressed SP-proteins among portions. SP-samples were subjected to a combination of SEC, 1-D SDS PAGE and NanoLC-ESI-MS/MS followed by functional bioinformatics analysis.The identified proteins were quantified from normalized LFQ intensity data. A total of 33,557 spectra corresponding to 8,189 peptides and 536 SP-proteins were identified with ≥ 95% Confidence (Unused Score > 1.3) and a false discovery rate (FDR) ≤ 1%. Of the 536 SP-proteins, 409 were identified in Sus Scrofa taxonomy and 374 of them were Biological Significance: This proteomic study provides the major characterization of the boar SP-proteome with more than 250 proteins first reported. The boar SP-proteome is described so that a spectral library can be built for relative 'label free' protein quantitation with SWATH approach. This proteomic profiling allows the creation of a publicly accessible database of the boar SP-proteome, as a first step for further understanding the role of SP-proteins in reproductive outcomes as well as for identification of biomarkers for sperm quality and fertility.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

Pérez-Patiño

Barranco

Parrilla

et al. 2016

Journal of Proteomics

Self Cite

102

View full text Add to dashboard Cite

show abstract

“…Recently, Barranco et al. () concluded his findings that measurement of seminal TAC ought to be a discriminative tool to prognosis fertility in breeding boars.…”

Section: Discussionmentioning

confidence: 99%

“…Our finding is in agreement with findings of others that found the low level of seminal TAC associated with male infertility (Eroglu et al, 2014;Khosravi, Valojerdi, Amanlou, Karimian, & Abolhassani, 2014;Mahfouz, Sharma, Sharma, Sabanegh, & Agarwal, 2009). Recently, Barranco et al (2015) concluded his findings that measurement of seminal TAC ought to be a discriminative tool to prognosis fertility in breeding boars.…”

Section: Discussionmentioning

confidence: 99%

Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo (Bubalus bubalis) bulls

Kumar

Saini

Kumar

et al. 2016

Reprod Domestic Animals

View full text Add to dashboard Cite

This study was attempted to identify subfertile bulls by quantifying the endogenous levels of osteopontin (OPN), total antioxidant capacity (TAC) and malondialdehyde (MDA) in seminal plasma of buffalo bulls. On the basis of conception rate, buffalo bulls were classified into two groups: high-fertile (conception rate >50%) and subfertile bulls (conception rate <40%). A total of 100 ejaculates (10 ejaculates from each bull) were collected through artificial vagina method. The concentration of OPN, TAC and catalase (CAT) of high-fertile bulls was found to be higher (p < .05) than that of subfertile bulls. Further, MDA level in seminal plasma was found to be lower (p < .05) in high-fertile bulls compared with subfertile bulls. The fertility status had no effect on the superoxide dismutase (SOD) concentration in seminal plasma of both the groups. The levels of OPN (r = .678, p = 0.013) and TAC (r = .648, p = .042) were found to be positively correlated with bull fertility and the level of MDA (r = -.718, p = .019) was found to be negatively correlated with bull fertility. However, the fertility of bulls was not found to be significantly correlated with SOD, CAT and sperm motility. In conclusion, seminal OPN, TAC and MDA tended to be more realistic in identification of subfertile bulls from breeding herds.

show abstract

“…These proteins can be used as biomarkers of sperm quality and fertility (Perez‐Patiño et al, ). In addition, the antioxidant enzymes present in the sperm‐rich fraction have a greater total antioxidant capacity (Barranco et al, ), where superoxide dismutase and paraoxonase‐1 are in higher concentrations (Li et al, ). The freezing/thawing of the first two fractions of the ejaculate presents a greater progressive motility and viability, where the antioxidants are involved in cryotolerance, reducing oxidative stress (Li et al, ).…”

Section: Seminal Plasmamentioning

confidence: 99%

Preservation of boar semen: An update

Pezo¹,

Romero

Zambrano³

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

Contents In the pork industry, artificial insemination and the storage of boar semen in liquid at 17°C are routinely applied to optimize the ejaculate and bring about rapid genetic changes that are reflected in the animal protein. Although the results are satisfactory, they are below what occurs with natural mating. It is currently possible to preserve boar semen with storage at 17°C and slow freezing, since to date there is only one study on vitrification, with negative results applicable only in the case of implementing an intracytoplasmic sperm injection. In both methods and due to the sensitivity of boar sperm to osmotic and temperature changes, there is a loss in the quality of the initial sample; however, slow freezing in boar semen has greater deleterious effects on the sample that are reflected in the pregnancy rates and number of live births. Therefore, only 1% of all inseminations are done with frozen semen. The aim of this review is to provide advances and results of studies conducted on the preservation of boar semen, delving more deeply into the critical points that each of the preservation techniques presents, including bacterial contamination, extender components, temperature, ice nucleation, use of additives in extenders and the main deleterious effects on sperm quality.

show abstract

High total antioxidant capacity of the porcine seminal plasma (SP-TAC) relates to sperm survival and fertility

Cited by 71 publications

References 31 publications

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

Characterization of the porcine seminal plasma proteome comparing ejaculate portions

Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo (Bubalus bubalis) bulls

Preservation of boar semen: An update

Contact Info

Product

Resources

About