High triacylglycerol turnover is required for efficient opening of stomata during heat stress in Arabidopsis

Korte, Pamela; Unzner, Amelie; Damm, Theresa; Berger, Susanne; Krischke, Markus; Mueller, Martin J.

doi:10.1111/tpj.16210

Cited by 11 publications

(3 citation statements)

References 60 publications

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“…Considering that low sink strength negatively affected tuber quality and yield in a heat-sensitive potato cultivar (Hastilestari et al, 2018), the cP2 approach may also improve stress tolerance and development in other Solanaceae. In fact, TAG turnover was recently shown to be required for efficient stomata opening during heat stress in Arabidopsis (Korte et al, 2023). Based on systems biology and metabolomic analyses, the establishment of a primed or pre-conditioned state has been suggested for improving crop plants (Tugizimana et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Metabolic priming in G6PDH isoenzyme‐replaced tobacco lines improves stress tolerance and seed yields via altering assimilate partitioning

Scharte,

Hassa,

Herrfurth

et al. 2023

The Plant Journal

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SUMMARYWe investigated the basis for better performance of transgenic Nicotiana tabacum plants with G6PDH‐isoenzyme replacement in the cytosol (Xanthi::cP2::cytRNAi, Scharte et al., 2009). After six generations of selfing, infiltration of Phytophthora nicotianae zoospores into source leaves confirmed that defence responses (ROS, callose) are accelerated, showing as fast cell death of the infected tissue. Yet, stress‐related hormone profiles resembled susceptible Xanthi and not resistant cultivar SNN, hinting at mainly metabolic adjustments in the transgenic lines. Leaves of non‐stressed plants contained twofold elevated fructose‐2,6‐bisphosphate (F2,6P2) levels, leading to partial sugar retention (soluble sugars, starch) and elevated hexose‐to‐sucrose ratios, but also more lipids. Above‐ground biomass lay in between susceptible Xanthi and resistant SNN, with photo‐assimilates preferentially allocated to inflorescences. Seeds were heavier with higher lipid‐to‐carbohydrate ratios, resulting in increased harvest yields ‐ also under water limitation. Abiotic stress tolerance (salt, drought) was improved during germination, and in floated leaf disks of non‐stressed plants. In leaves of salt‐watered plants, proline accumulated to higher levels during illumination, concomitant with efficient NADP(H) use and recycling. Non‐stressed plants showed enhanced PSII‐induction kinetics (upon dark–light transition) with little differences at the stationary phase. Leaf exudates contained 10% less sucrose, similar amino acids, but more fatty acids – especially in the light. Export of specific fatty acids via the phloem may contribute to both, earlier flowering and higher seed yields of the Xanthi‐cP2 lines. Apparently, metabolic priming by F2,6P2—combined with sustained NADP(H) turnover—bypasses the genetically fixed growth–defence trade‐off, rendering tobacco plants more stress‐resilient and productive.

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Section: Discussionmentioning

confidence: 99%

Metabolic priming in G6PDH isoenzyme‐replaced tobacco lines improves stress tolerance and seed yields via altering assimilate partitioning

Scharte,

Hassa,

Herrfurth

et al. 2023

The Plant Journal

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“…An aliquot (50 µL) of the derivatized AA extract was transferred to UPLC vials. AAs were measured as described previously ( 54 ).…”

Section: Methodsmentioning

confidence: 99%

S ₁ basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs

Kreisz,

Hellens,

Fröschel

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Plants tightly control growth of their lateral organs, which led to the concept of apical dominance. However, outgrowth of the dormant lateral primordia is sensitive to the plant’s nutritional status, resulting in an immense plasticity in plant architecture. While the impact of hormonal regulation on apical dominance is well characterized, the prime importance of sugar signaling to unleash lateral organ formation has just recently emerged. Here, we aimed to identify transcriptional regulators, which control the trade-off between growth of apical versus lateral organs. Making use of locally inducible gain-of-function as well as single and higher-order loss-of-function approaches of the sugar-responsive S 1 -basic-leucine-zipper (S 1 -bZIP) transcription factors, we disclosed their largely redundant function in establishing apical growth dominance. Consistently, comprehensive phenotypical and analytical studies of S 1 -bZIP mutants show a clear shift of sugar and organic nitrogen (N) allocation from apical to lateral organs, coinciding with strong lateral organ outgrowth. Tissue-specific transcriptomics reveal specific clade III SWEET sugar transporters, crucial for long-distance sugar transport to apical sinks and the glutaminase GLUTAMINE AMIDO-TRANSFERASE 1_2.1 , involved in N homeostasis, as direct S 1 -bZIP targets, linking the architectural and metabolic mutant phenotypes to downstream gene regulation. Based on these results, we propose that S 1 -bZIPs control carbohydrate (C) partitioning from source leaves to apical organs and tune systemic N supply to restrict lateral organ formation by C/N depletion. Knowledge of the underlying mechanisms controlling plant C/N partitioning is of pivotal importance for breeding strategies to generate plants with desired architectural and nutritional characteristics.

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“…An aliquot (50 µl) of the derivatized amino acid extract was transferred to UPLC vials. AAs were measured as described previously 52 .…”

Section: Amino Acid Measurementsmentioning

confidence: 99%

S₁basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs

Kreisz,

Hellens,

Fröschel

et al. 2023

Preprint

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Plants exhibit an immense plasticity in their architecture. While the impact of hormonal regulation is well-characterised, the importance of sugar-signalling has just recently emerged. Here, we addressed which sugar-signalling components mediate the trade-off between growth of apical versus lateral meristems and how they control organ sink-strength. Thereby, we unravelled a novel developmental function of the sugar-controlled S1 basic-leucine-zipper (S1-bZIP) transcription factors in establishing global source-sink interactions. Applying comprehensive molecular, analytical, and genetic approaches, we demonstrate that S1-bZIPs operate in a redundant manner to control tissue-specific expression of defined SWEET sugar-transporters and the GAT1_2.1 glutaminase. By these means, S1-bZIPs control carbohydrate (C)-channelling from source leaves to apical shoot and root organs and tune systemic organic nitrogen (N)-supply to restrict lateral organ formation by C/N depletion. Knowledge of the underlying mechanisms controlling plant C/N partitioning is of pivotal importance for breeding strategies to generate plants with desired architectural and nutritional characteristics.

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High triacylglycerol turnover is required for efficient opening of stomata during heat stress in Arabidopsis

Cited by 11 publications

References 60 publications

Metabolic priming in G6PDH isoenzyme‐replaced tobacco lines improves stress tolerance and seed yields via altering assimilate partitioning

Metabolic priming in G6PDH isoenzyme‐replaced tobacco lines improves stress tolerance and seed yields via altering assimilate partitioning

S ₁ basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs

S₁basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs

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High triacylglycerol turnover is required for efficient opening of stomata during heat stress in Arabidopsis

Cited by 11 publications

References 60 publications

Metabolic priming in G6PDH isoenzyme‐replaced tobacco lines improves stress tolerance and seed yields via altering assimilate partitioning

Metabolic priming in G6PDH isoenzyme‐replaced tobacco lines improves stress tolerance and seed yields via altering assimilate partitioning

S 1 basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs

S1basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs

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S ₁ basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs

S₁basic leucine zipper transcription factors shape plant architecture by controlling C/N partitioning to apical and lateral organs