High-value products from plants: the challenges of process optimization

Fischer, Rainer; Vasil'ev, N. P.; Twyman, Richard M.; Schillberg, Stefan

doi:10.1016/j.copbio.2014.12.018

Cited by 66 publications

(41 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Marin Viegas et al [60], a transient expression system for the production of human TG2 in N. benthamiana leaves was optimized, and the reactivity of plant-produced TG2 in a CD screening test was evaluated. Hence, transient expression performed in contained facilities satisfies good manufacturing practices, and quick expression can avoid the time-consuming stable transformation [58,61]. In a comparison of productivity in terms of biomass production, Hiatt and Pauly [62] reported that grams of product may take only two weeks plus a few weeks more.…”

Section: Plant-based Expression System For Vaccine Developmentmentioning

confidence: 99%

The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B

Joung

Park

Moon

et al. 2016

IJMS

View full text Add to dashboard Cite

Disease prevention through vaccination is considered to be the greatest contribution to public health over the past century. Every year more than 100 million children are vaccinated with the standard World Health Organization (WHO)-recommended vaccines including hepatitis B (HepB). HepB is the most serious type of liver infection caused by the hepatitis B virus (HBV), however, it can be prevented by currently available recombinant vaccine, which has an excellent record of safety and effectiveness. To date, recombinant vaccines are produced in many systems of bacteria, yeast, insect, and mammalian and plant cells. Among these platforms, the use of plant cells has received considerable attention in terms of intrinsic safety, scalability, and appropriate modification of target proteins. Research groups worldwide have attempted to develop more efficacious plant-derived vaccines for over 30 diseases, most frequently HepB and influenza. More inspiring, approximately 12 plant-made antigens have already been tested in clinical trials, with successful outcomes. In this study, the latest information from the last 10 years on plant-derived antigens, especially hepatitis B surface antigen, approaches are reviewed and breakthroughs regarding the weak points are also discussed.

show abstract

Section: Plant-based Expression System For Vaccine Developmentmentioning

confidence: 99%

The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B

Joung

Park

Moon

et al. 2016

IJMS

View full text Add to dashboard Cite

show abstract

“…A theoretical technoeconomic case study showed that plants can be highly competitive production platforms for biopharmaceuticals (Tuse et al 2014). Compliance with good manufacturing practices required for all products entering clinical development (Fischer et al 2012;Yusibov et al 2011) and optimization of production processes leading to a reduction of downstream processing-related costs will help to commercialize more plant-derived pharmaceuticals in the future (Fischer et al 2013(Fischer et al , 2015.…”

Section: Discussionmentioning

confidence: 99%

Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems

et al. 2016

View full text Add to dashboard Cite

Dengue fever is a disease in many parts of the tropics and subtropics and about half the world's population is at risk of infection according to the World Health Organization. Dengue is caused by any of the four related dengue virus serotypes DEN-1, -2, -3 and -4, which are transmitted to people by Aedes aegypti mosquitoes. Currently there is only one vaccine (Dengvaxia Ò ) available (limited to a few countries) on the market since 2015 after half a century's intensive efforts. Affordable and accessible vaccines against dengue are hence still urgently needed. The dengue envelop protein domain III (EDIII), which is capable of eliciting serotype-specific neutralizing antibodies, has become the focus for subunit vaccine development. To contribute to the development of an accessible and affordable dengue vaccine, in the current study we have used plant-based vaccine production systems to generate a dengue subunit vaccine candidate in tobacco. Chloroplast genome engineering was applied to express serotypespecific recombinant EDIII proteins in tobacco chloroplasts using both constitutive and ethanol-inducible expression systems. Expression of a tetravalent antigen fusion construct combining EDIII polypeptides from all four serotypes was also attempted. Transplastomic EDIIIexpressing tobacco lines were obtained and homoplasmy was verified by Southern blot analysis. Northern blot analyses showed expression of EDIII antigen-encoding genes. EDIII protein accumulation levels varied for the different recombinant EDIII proteins and the different expression systems, and reached between 0.8 and 1.6 % of total cellular protein. Our study demonstrates the suitability of the chloroplast compartment as a production site for an EDIII-based vaccine candidate against dengue fever and presents a Gateway Ò plastid transformation vector for inducible transgene expression.

show abstract

“…The classical molecular farming technology is based on genetic modification of plants for recombinant protein expression. However, both methods requires growing of whole plants under strictly controlled environmental conditions into contained greenhouses, complying with the strict regulatory standards [36]. However, both methods requires growing of whole plants under strictly controlled environmental conditions into contained greenhouses, complying with the strict regulatory standards [36].…”

Section: Molecular Farming For Production Of Recombinant Proteinsmentioning

confidence: 99%

“…Plants are excellent production matrixes for expression of recombinant proteins with important pharmaceutical properties [36]. This powerful recombinant protein expression technique, known as "molecular farming" have been widely used for the production of vaccines [37][38][39], cytokines [40], and even for production of therapeutic protein for human use [5,41,42].…”

Section: Molecular Farming For Production Of Recombinant Proteinsmentioning

confidence: 99%

Plant cell culture as emerging technology for production of active cosmetic ingredients

Georgiev

Slavov

Vasileva

et al. 2018

Engineering in Life Sciences

View full text Add to dashboard Cite

Plants have always been the main source for active cosmetic ingredients, having proven health beneficial effects on human, such as anti‐aging, antioxidant, anti‐inflammatory, UV‐protective, anti‐cancer, anti‐wrinkle, skin soothing, whitening, moisturizing, etc. Extracts from herbal, aromatic and/or medicinal plants have been widely used as effective active ingredients in cosmeceuticals or nutricosmetics, especially in products for topical application and skin‐care formulations. However, over the past decade, there has been an increasing interest to plant cell culture – derived active cosmetic ingredients. These are “new generation” of high quality natural products, produced by the modern plan biotechnology methods, which usually showed stronger activities than the plant extracts obtained by the classical methods. In this review, the advantages and the current progress in plant cell culture technology for the production of active cosmetic ingredients have been summarized, and discussed in details within a presented case study for calendula stem cell product development.

show abstract

High-value products from plants: the challenges of process optimization

Cited by 66 publications

References 50 publications

The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B

The Last Ten Years of Advancements in Plant-Derived Recombinant Vaccines against Hepatitis B

Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems

Plant cell culture as emerging technology for production of active cosmetic ingredients

Contact Info

Product

Resources

About