High‐value Recombinant Protein Production in Microalgae

Barrera, Daniel J.; Mayfield, Stephen P.

doi:10.1002/9781118567166.ch27

Cited by 31 publications

(26 citation statements)

References 91 publications

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“…Over the last 70 years, C. reinhardtii has become the flagship alga for laboratory studies and genetic manipulation. The eukaryotic green alga has three modifiable genomes and is capable of producing a wide variety of protein products (Rosales-Mendoza et al, 2012;Barrera and Mayfield, 2013;Rasala et al, 2013b). The efficient manufacture of these products at commercial viability will require a myriad of genetic tools to enhance protein accumulation and bioactivity ( Figure 1).…”

Section: Chloroplast Engineeringmentioning

confidence: 99%

“…To date, the chloroplast genome has been the primary target for engineering protein production, predominantly because it readily performs homologous recombination and is easily transformed (Boynton et al, 1988). Typically, recombinant protein expressed from the chloroplast accounts for 1-10% of total protein (Almaraz-Delgado et al, 2013;Barrera and Mayfield, 2013;Rasala and Mayfield, 2014).…”

Section: Chloroplast Engineeringmentioning

confidence: 99%

“…Production of recombinant proteins in C. reinhardtii has been a fruitful area of research in recent years. Several recent articles have thoroughly reviewed the plethora of protein products that have been successfully expressed within C. reinhardtii (Almaraz-Delgado et al, 2013;Barrera and Mayfield, 2013;Rasala and Mayfield, 2014). To date, 34 different protein co-products have been produced in algae, 29 of which were produced in C. reinhardtii.…”

Section: High-value Bio-productsmentioning

confidence: 99%

“…One near-term solution is to couple biofuel production with high-value co-products to increase the commercial value of the entire algal biomass. Current highvalue bio-products produced in algae include industrial and therapeutic proteins as well as nutraceuticals and other high-value small molecules (Almaraz-Delgado et al, 2013;Barrera and Mayfield, 2013;Rasala and Mayfield, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Chlamydomonas reinhardtii has led the field in the development of molecular tools for strain selection and engineering for green alga. By far, more recombinant proteins have been expressed in C. reinhardtii than all other algal species combined (Almaraz-Delgado et al, 2013;Barrera and Mayfield, 2013;Rasala and Mayfield, 2014). Studies in C. reinhardtii have also helped elucidate the molecular mechanisms behind algal lipid and hydrogen metabolism (Merchant et al, 2011;Torzillo and Seibert, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Chlamydomonas as a model for biofuels and bio‐products production

et al. 2015

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SUMMARY Developing renewable energy sources is critical to maintaining the economic growth of the planet while protecting the environment. First generation biofuels focused on food crops like corn and sugarcane for ethanol production, and soybean and palm for biodiesel production. Second generation biofuels based on cellulosic ethanol produced from terrestrial plants, has received extensive funding and recently pilot facilities have been commissioned, but to date output of fuels from these sources has fallen well short of what is needed. Recent research and pilot demonstrations have highlighted the potential of algae as one of the most promising sources of sustainable liquid transportation fuels. Algae have also been established as unique biofactories for industrial, therapeutic, and nutraceutical co-products. Chlamydomonas reinhardtii’s long established role in the field of basic research in green algae has paved the way for understanding algal metabolism and developing genetic engineering protocols. These tools are now being utilized in C. reinhardtii and in other algal species for the development of strains to maximize biofuels and bio-products yields from the lab to the field.

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Section: Chloroplast Engineeringmentioning

confidence: 99%

Section: Chloroplast Engineeringmentioning

confidence: 99%

Section: High-value Bio-productsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chlamydomonas as a model for biofuels and bio‐products production

et al. 2015

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Alga‐Made Anti‐Hepatitis B Antibody Binds to Human Fcγ Receptors

Vanier

Stelter

Vanier

et al. 2017

Biotechnology Journal

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Microalgae are unicellular eukaryotic organisms which represent an emerging alternative to other cell biofactories commonly used to produce monoclonal antibodies. Microalgae display several biotechnological advantages such as their rapid growth rate and their phototrophic lifestyle allowing low production costs as protein expression is solar-fueled. Recently, a fully assembled recombinant IgG antibody directed against Hepatitis B surface antigen is produced and secreted in the culture medium of the diatom Phaeodactylum tricornutum. A biochemical characterization of this recombinant antibody demonstrated that the Asn-297 is N-glycosylated by oligomannosides. In the immune system, antibodies interact with effector molecules and cells through their Fc part and the recognition of Fcγ receptors (FcγR) which are important for inducing phagocytosis of opsonized microbes. Interactions between IgG and FcγR are influenced by the N-glycan structures present on the Asn-297. In this study, the authors characterized the binding capacity of the anti-hepatitis B recombinant IgG produced in P. tricornutum to two human Fcγ receptors (FcγRI and IIIa) using a cellular binding assay and surface plasmon resonance (SPR). This allowed us to demonstrate that the alga-made antibody is able to bind FcγRI with a reduced affinity and engages FcyRIIIa with 3-times higher affinity compared to a control human IgG1.

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Chlorella vulgaris growth, pigment and lipid accumulation: effect of progressive light and hydrogen peroxide exposure

Cunha

Sátiro

Escobar

et al. 2022

J of Chemical Tech & Biotech

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BACKGROUND Chlorella vulgaris is being explored for several applications such as biodiesel production, nutrient supplementation, animal feed and pharmaceutical products. In this study, the performance of a batch culture under progressive light intensity and the effect of hydrogen peroxide (H2O2) concentration on lipid accumulation were investigated. RESULTS Under progressive light intensity (60 to 300 μmol m−2 s−1), a 43% biomass increase was achieved in comparison with the control light conditions (60 μmol m−2 s−1), whereas a 300 μmol m−2 s−1 light intensity caused growth inhibition to the culture. In addition, an increase in carotenoid content from 0.102 mg g−1 biomass (control) to 0.460 mg g−1 (progressive light) and lipid accumulation from 31.0 mg g−1 (control) to 36.5 mg g−1 (progressive light) was observed. However, the moderate illumination conditions (60 μmol m−2 s−1) induced a very significant increase of chlorophyll b, regarding the progressive light mode, increasing the photosynthetic efficiency. After the growth phase, the biomass was subjected to stress conditions by adding H2O2 to the culture medium at different concentrations: 0.75 mmol L−1 increased the lipid content of the biomass from 3.6% to 10.3% in a 3‐day test, reaching lipid productivity of 70.4 mg L−1 day−1, an increase of ≈40% compared to the phosphate starvatiod for the same culture time. CONCLUSION The progressive light system allowed an increase in biomass and lipid accumulation. Together with the exposure to H2O2, this work provides a new approach for lipid and pigment production from microalgae. © 2022 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

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High‐value Recombinant Protein Production in Microalgae

Cited by 31 publications

References 91 publications

Chlamydomonas as a model for biofuels and bio‐products production

Chlamydomonas as a model for biofuels and bio‐products production

Alga‐Made Anti‐Hepatitis B Antibody Binds to Human Fcγ Receptors

Chlorella vulgaris growth, pigment and lipid accumulation: effect of progressive light and hydrogen peroxide exposure

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