High variations of methanogenic microorganisms drive full-scale anaerobic digestion process

Zhang, Qiuting; Wang, Mengmeng; Ma, Xingyu; Gao, Qun; Wang, Tengxu; Shi, Xuchuan; Zhou, Jizhong; Zuo, Jiane; Yang, Yunfeng

doi:10.1016/j.envint.2019.03.005

Cited by 68 publications

(35 citation statements)

References 57 publications

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“…The two remaining bacterial genera (Smithella and Treponema), found in all samples, represent acetogenic bacteria. Smithella (2.5-7.4%, highest in B_FD) are recognized as mesophilic, syntrophic propionate-oxidizing bacteria producing acetate, while Treponema (0.5-6.8%, highest in G_SD) is one of the homoacetogenic bacteria, also producing acetate using H 2 and CO 2 [11,[23][24][25]. Their common presence induces universal acetate production through syntrophic reaction and homoacetogenesis during AD.…”

Section: Bacterial Structurementioning

confidence: 99%

“…Recent developments in high-throughput sequencing tools have enabled the sequencing of large microbial communities in complex biological samples at low cost and with short analytical times. Therefore, the number of studies focused on microbial communities originating from laboratory, pilot, and full-scale AD plants, and using 454 pyrosequencing of the 16S rRNA gene, are increasing [8][9][10][11]. A comprehensive overview of the microbial communities involved in AD is necessary to understand the influence of particular microorganisms on the AD process under specific conditions.…”

Section: Introductionmentioning

confidence: 99%

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Microbiome of Seven Full-Scale Anaerobic Digestion Plants in South Korea: Effect of Feedstock and Operational Parameters

2021

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In this study, the microbiomes linked with the operational parameters in seven mesophilic full-scale AD plants mainly treating food waste (four plants) and sewage sludge (three plants) were analyzed. The results obtained indicated lower diversity and evenness of the microbial population in sludge digestion (SD) plants compared to food digestion (FD) plants. Candidatus Accumulibacter dominated (up to 42.1%) in SD plants due to microbial immigration from fed secondary sludge (up to 89%). Its potential activity in SD plants was correlated to H2 production, which was related to the dominance of hydrogenotrophic methanogens (Methanococcus). In FD plants, a balance between the hydrogenotrophic and methylotrophic pathways was found, while Flavobacterium and Levilinea played an important role during acidogenesis. Levilinea also expressed sensitivity to ammonia in FD plants. The substantial differences in hydraulic retention time (HRT), organic loading rate (OLR), and total ammonium nitrogen (TAN) among the studied FD plants did not influence the archaeal methane production pathway. In addition, the bacterial genera responsible for acetate production through syntrophy and homoacetogenesis (Smithella, Treponema) were present in all the plants studied.

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Section: Bacterial Structurementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbiome of Seven Full-Scale Anaerobic Digestion Plants in South Korea: Effect of Feedstock and Operational Parameters

2021

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“…Methanogenic microorganisms belonging to the domain of Archaea and participating in the AD process are of key importance for biogas production and high CH 4 yield. Methanogens are particularly sensitive to antimicrobials, whose presence in substrate (cattle manure) used in AD can slow down the rate of their growth and decrease their metabolic activity [6]. The risk of the occurrence of drug resistance (DR) and increasing number of antibiotic resistance genes (ARGs) in digestate from cattle manure, an AD by-product, due to the widespread use of antibiotics in veterinary medicine and agriculture, is a significant problem [7].…”

Section: Introductionmentioning

confidence: 99%

“…The risk of the occurrence of drug resistance (DR) and increasing number of antibiotic resistance genes (ARGs) in digestate from cattle manure, an AD by-product, due to the widespread use of antibiotics in veterinary medicine and agriculture, is a significant problem [7]. There is also a thread of the emergence of selection pressure and diffusion of resistant strains of microorganisms as well as their MGEs (mobile genetic elements) in digestate [6].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure

et al. 2021

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This study explored the effect of eight antimicrobials on the efficiency of biogas production in the anaerobic digestion (AD) process of cattle manure. The microbiome involved in AD, presence and number of genes mcrA, MSC and MST specific for Archaea, and antibiotic resistance genes (ARGs) concentration in digestate (D) were examined. Supplementation of antibiotics to substrate significantly lowered biogas production. Amoxicillin caused a 75% decrease in CH4 production in comparison with the control samples. Enrofloxacin, tetracycline, oxytetracycline, and chlortetracycline reduced the amount of biogas produced by 36, 39, 45 and 53%, respectively. High-throughput sequencing of 16S rRNA results revealed that bacteria dominated the Archaea microorganisms in all samples. Moreover, antibiotics led to a decrease in the abundance of the genes mcrA, MSC, MST, and induced an increase in the number of tetracyclines resistance genes. Antibiotics decreased the efficiency of the AD process and lowered the quantity of CH4 obtained, while stimulating an increase in the number of ARGs in D. This work reveals how antimicrobials affect the cattle manure AD process and changes in microbial biodiversity, number of functional genes and ARGs in the digestate due to drugs exposure. It also, provides useful, practical information about the AD process.

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“…As a matter of fact, there are three basic behaviors could be distinguished in the microbial dynamics under harsh conditions, namely, 1) resistance in which microbes can deal with changes and thus no composition variation will take place, 2) resilience in which microorganisms able to recover after upset and 3) redundant in which new microbes have the ability to replace the disturbed populations (Carballa et al, 2015). Even though microorganisms involved in different AD stages are functionally redundant (De Vrieze et al, 2017;Zhang et al, 2019), the distinct members that are able to replace each other upon operational disturbances need more investigation.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic digestion concert of agro-food wastes and the correlated microbial population dynamics under suboptimal, well-performed and disturbed states

Yin¹,

Mahdy²,

Liu³

et al.

Authorea

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The comparison among microbial interactions during the stable performance of anaerobic digestion (AD) and the process disturbances is still lack and could limit the prediction of process failure and the possible recovery. This study aimed at characterizing the process performance and microbial communities' profiles during the stable and disturbed states of long-term thermophilic AD process fed with agro-food wastes. The disturbances were induced in two stages, firstly under a stepwise increase of organic loading rate (OLR), and then through the reduction in hydraulic retention time (HRT). Volatile fatty acids (VFAs) only accumulated (4730 mg L-1) when OLR increased to 17 g VS L-1 d-1, and consequently methane yield deteriorated by 47%, implying process overloading and thus AD process was partially inhibited. Process disturbances led to 30% reduction in relative abundance of Defluviitoga and Methanoculleus which were partially displaced by Clostridium and Methanomassiliicoccus, implying that the process acidification immediately reflected on microbial profile and the microbes were functionally redundant. Microorganisms' washout was the main reason behind methane yield drop under finite digestion time (1.5d). Microbial profiles shaping showed the robustness of AD process due to the functionally redundant microorganisms and could be strategically used to control and optimize AD process.

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High variations of methanogenic microorganisms drive full-scale anaerobic digestion process

Cited by 68 publications

References 57 publications

Microbiome of Seven Full-Scale Anaerobic Digestion Plants in South Korea: Effect of Feedstock and Operational Parameters

Microbiome of Seven Full-Scale Anaerobic Digestion Plants in South Korea: Effect of Feedstock and Operational Parameters

The Effect of Antibiotics on Mesophilic Anaerobic Digestion Process of Cattle Manure

Anaerobic digestion concert of agro-food wastes and the correlated microbial population dynamics under suboptimal, well-performed and disturbed states

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