High Visfatin Expression in Breast Cancer Tissue Is Associated with Poor Survival

Lee, Yi Chen; Yang, Yi‐Hsin; Su, Jui Hsin; Chang, Hsueh Ling; Hou, Ming‐Feng; Yuan, Shyng‐Shiou F.

doi:10.1158/1055-9965.epi-11-0399

Cited by 74 publications

(63 citation statements)

References 41 publications

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“…Exogenous PBEF1/NAMPT/Visfatin has proangiogenic activity, regulates endothelial IL6 production through Stat3 in vitro [52] and can induce the mTOR pathway in HUVEC cells [53]. PBEF1 is upregulated at the mRNA level in various tumor types such as prostate cancers [54] and correlated with poor survival in breast cancer [55]. Recently, an adipogenic signature was identified in CCRCC by comparison of low stage tumours and normal kidney, as a model of renal tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

The experimental renal cell carcinoma model in the chick embryo

et al. 2012

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The clear cell subtype of renal carcinoma (CCRCC) is highly vascularized and despite a slow progression rate, it is potentially a highly aggressive tumor. Although a doubling of median progression-free survival in CCRCC patients treated by targeted therapies has been observed, the fact that tumors escape after anti-VEGF treatment suggests alternative pathways. The chick chorioallantoic membrane (CAM) is a well-established model, which allows in vivo studies of tumor angiogenesis and the testing of anti-angiogenic molecules. However, only a few data exist on CCRCC grafted onto CAM. We aimed to validate herein the CAM as a suitable model for studying the development of CCRCC and the interactions with the surrounding stroma. Our study uses both CCRCC cell lines and fresh tumor samples after surgical resection. We demonstrate that in both cases CCRCC can be grafted onto the CAM, to survive and to induce an angiogenic process. We further provide insights into the transcriptional regulation of the model by performing a differential analysis of tumor-derived and stroma-derived transcripts.

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Section: Discussionmentioning

confidence: 99%

The experimental renal cell carcinoma model in the chick embryo

et al. 2012

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“…Tumor tissue samples of the patients were obtained from formalin-fixed and paraffin-embedded tissue blocks for the construction of tissue microarray by an Alphelys BoostArrayer device (Plaisir) as described previously (6). Five-micrometer sections from the tissue microarray were obtained using a microtome and immunohistochemically stained for determination of protein expression.…”

Section: Tissue Microarraymentioning

confidence: 99%

“…The procedure of immunohistochemical staining was described previously (6). The primary antibodies used for immunohistochemistry in this study included the mouse monoclonal anti-ER, anti-PR, and anti-HER2 antibodies from DAKO (Glostrup), rabbit polyclonal anti-phospho-c-Abl (Y393/412) and antivimentin antibodies from GeneTex (Irvine), rabbit monoclonal anti-phospho-STAT3 (Y705) antibody from Cell Signaling Technology, and mouse monoclonal anti-cytokeratin 18 antibody from Leica.…”

Section: Immunohistochemistrymentioning

confidence: 99%

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Extracellular Visfatin-Promoted Malignant Behavior in Breast Cancer Is Mediated Through c-Abl and STAT3 Activation

Hung

Hou

et al. 2016

Clinical Cancer Research

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Purpose: Visfatin is an adipocytokine involved in cellular metabolism, inflammation, and cancer. This study investigated the roles of extracellular visfatin in breast cancer, and explored underlying mechanisms in clinical and experimental settings.Experimental Design: Associations of serum visfatin with clinicopathologic characteristics and patient survival were assessed with Cox regression models and Kaplan-Meier analyses. Effects of extracellular visfatin on cultured breast cancer cells were examined, followed by in vivo investigation of tumor growth and metastasis in xenograft animal models. Imatinib and Stattic were used to inhibit c-Abl and STAT3 activation, respectively.Results: Breast cancer patients with high serum visfatin levels were associated with advanced tumor stage, increased tumor size and lymph node metastasis, and poor survival. Elevated phosphorylation of c-Abl and STAT3 in breast tumor tissues were correlated with high serum visfatin levels in patients. Visfatinpromoted in vitro cell viability and metastatic capability were suppressed by imatinib (c-Abl inhibitor) and Stattic (STAT3 inhibitor). Increased in vivo cell invasiveness was observed in zebrafish xenografted with visfatin-pretreated breast cancer cells. Tumor growth and lung metastasis occurred in visfatin-administered mice xenografted with breast cancer cells. Tail vein-injected mice with visfatin-pretreated breast cancer cells showed increased lung metastasis, which was suppressed by imatinib.Conclusions: Serum visfatin levels in breast cancer patients reveal potential prognostic values, and our findings that visfatin promoted breast cancer through activation of c-Abl and STAT3 may provide an important molecular basis for future design of targeted therapies that take into account different serum visfatin levels in breast cancer.

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“…To this end, MDA-MB-231 cells were selected because FK866 was found to minimally affect their growth (24,20, and 27% growth inhibition at 72 h in response to 10 nM, 100 nM, and 1 M FK866, respectively), while, at the same time, reducing intracellular NAD ϩ by more than 50%. Conversely, BT549 and MDA-MD-468 were more sensitive to the growth-inhibiting effects of this drug (for BT549, 51, 54, and 67% growth inhibition at 72 h in response to 10 nM, 100 nM, and 1 M FK866, respectively; for MDA-MD-468, 32, 38, and 41% growth inhibition at 72 h in response to 10 nM, 100 nM, and 1 M FK866, respectively) and, therefore, were not selected for these experiments.…”

Section: Nampt Expression In Bc Is Associated With Estrogen Receptornmentioning

confidence: 99%

Nicotinamide Phosphoribosyltransferase Promotes Epithelial-to-Mesenchymal Transition as a Soluble Factor Independent of Its Enzymatic Activity

Soncini

Caffa

Zoppoli

et al. 2014

Journal of Biological Chemistry

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High Visfatin Expression in Breast Cancer Tissue Is Associated with Poor Survival

Cited by 74 publications

References 41 publications

The experimental renal cell carcinoma model in the chick embryo

The experimental renal cell carcinoma model in the chick embryo

Extracellular Visfatin-Promoted Malignant Behavior in Breast Cancer Is Mediated Through c-Abl and STAT3 Activation

Nicotinamide Phosphoribosyltransferase Promotes Epithelial-to-Mesenchymal Transition as a Soluble Factor Independent of Its Enzymatic Activity

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