High water-soluble curcuminoids-rich extract regulates osteogenic differentiation of MC3T3-E1 cells: Involvement of Wnt/β-catenin and BMP signaling pathway

Pengjam, Yutthana; Syazwani, Nurul; Inchai, Jakkapong; Numit, Amornkan; Yodthong, Thanintorn; Pitakpornpreecha, Thanawat; Panichayupakaranant, Pharkphoom

doi:10.1016/j.chmed.2021.01.003

Cited by 10 publications

(9 citation statements)

References 30 publications

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“…The increase in the number of osteoblasts on the 7th day is because osteoblasts are still undergoing cell proliferation, this will increase the number of osteoblasts, thus accelerating bone formation. 15,17,25 The results in this study show the application of a 15% concentration of ramania leaf extract gel in the post-extraction socket was better at increasing the number of osteoblasts than positive and negative controls. This is due to the presence of secondary metabolites in ramania leaves which can be used to optimize wound healing and increase the number of osteoblasts.…”

Section: Discussionmentioning

confidence: 69%

The Effect of Ramania Leaves Extract Gel (Bouea macrophylla Griff) on the Number of Osteoblast (in vivo Study of Post Extraction in Wistar Rats (Rattus norvegicus))

Taufiqurrahman,

Gupita,

Oktiani

et al. 2024

Pharmacogn J.

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Ramania leaves extract contains secondary metabolite compounds such as flavonoids, steroids, phenols and terpenoids which can be used as an alternative medicine for socket wound healing. Flavonoids have anti-inflammatory and antioxidant properties that can accelerate wound healing and can stimulate the formation of osteoblasts. Analyzing the differences in the number of osteoblasts in the negative control group, positive control given aloe vera gel 15% and ramania leaf extract gel 15% on day 5, 7 and 14. This study used true experimental design with posttest-only control design, using 27 rats divide into 3 groups. The experimental animals were sacrificed on days 5, 7 and 14 for histopathological observations. Two-Way ANOVA test results obtained p value = 0.013 <0.05, which indicates that there is a significant difference between the use of 15% ramania leaf extract gel 15% and the number of osteoblasts on days 5, 7 and 14. Bonferroni's Post Hoc test showed a difference. The number of osteoblasts was significant among the negative control group with ramania leaf extract gel 15%. Ramania leaf extract gel 15% was proven to be able to increase the number of osteoblasts and has the potential as an alternative medicine for socket healing after tooth extraction.

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Section: Discussionmentioning

confidence: 69%

The Effect of Ramania Leaves Extract Gel (Bouea macrophylla Griff) on the Number of Osteoblast (in vivo Study of Post Extraction in Wistar Rats (Rattus norvegicus))

Taufiqurrahman,

Gupita,

Oktiani

et al. 2024

Pharmacogn J.

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“…The MHAP NPs treatments at less than 10 μg/mL significantly increased the ALP activity ( Figure 6 A,B) compared to the untreated control on day 14. In MC3T3-E1 cells, elevated ALP enzyme activity is considered characteristic of the initial stage of osteoblast development [ 64 ]. The outcomes are highly correlated and in agreement with those of the control samples.…”

Section: Resultsmentioning

confidence: 99%

Magnetic Hydroxyapatite Composite Nanoparticles for Augmented Differentiation of MC3T3-E1 Cells for Bone Tissue Engineering

et al. 2023

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Progressive aging harms bone tissue structure and function and, thus, requires effective therapies focusing on permanent tissue regeneration rather than partial cure, beginning with regenerative medicine. Due to advances in tissue engineering, stimulating osteogenesis with biomimetic nanoparticles to create a regenerative niche has gained attention for its efficacy and cost-effectiveness. In particular, hydroxyapatite (HAP, Ca10(PO4)6(OH)2) has gained significant interest in orthopedic applications as a major inorganic mineral of native bone. Recently, magnetic nanoparticles (MNPs) have also been noted for their multifunctional potential for hyperthermia, MRI contrast agents, drug delivery, and mechanosensitive receptor manipulation to induce cell differentiation, etc. Thus, the present study synthesizes HAP-decorated MNPs (MHAP NPs) via the wet chemical co-precipitation method. Synthesized MHAP NPs were evaluated against the preosteoblast MC3T3-E1 cells towards concentration-dependent cytotoxicity, proliferation, morphology staining, ROS generation, and osteogenic differentiation. The result evidenced that MHAP NPs concentration up to 10 µg/mL was non-toxic even with the time-dependent proliferation studies. As nanoparticle concentration increased, FACS apoptosis assay and ROS data showed a significant rise in apoptosis and ROS generation. The MC3T3-E1 cells cocultured with 5 µg/mL MHAP NPs showed significant osteogenic differentiation potential. Thus, MHAP NPs synthesized with simple wet chemistry could be employed in bone regenerative therapy.

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“…Stimulate. In the related studies of continuous static pressure on MC3T3-E1 cells, OC, ColⅠ, OPN, RANKL, NOX4, OPG, osteocalcin, ADAM28, TD, ALP, Runx2, Wnt1, DKK-1, and other genes and their Protein expression is the main research direction (Song, 2021;Pengjam, 2021;You et al, 2001;Zhao et al, 2021b). The osteoblasts responsible for bone formation activities are differentiated from bone marrow mesenchymal stem cells.…”

Section: Discussionmentioning

confidence: 99%

Study on complications of osteoporosis based on network pharmacology

Song

Zhang²,

Jiang³

et al. 2022

Front. Genet.

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Osteoporosis is a serious threat to human life. Guben Zenggu Granule is an empirical prescription for clinical treatment of osteoporosis. MC3T3-E1 cells are mouse osteogenic precursor cells with osteogenic differentiation, and are classic cells for studying bone metabolism and osteogenic mechanism, as well as mechanical stimulation sensitive cells. Therefore, it can be inferred that Guben Zenggu granule can repair MC3T3-E1 cells under continuous static pressure overload. This study aims to through the network of pharmacology and gene sequencing method, reveal thrift increase bone particles under the condition of continuous static pressure overload on osteogenesis mechanism of MC3T3-E1 cells. In the process of analysis, from a variety of 98 compounds was predicted in the database, a collection of 474 goals, a total of 29,164 difference between two groups of genes. Then, construction of composite targets between cells and predict targets and protein - protein interaction networks, and through the cluster analysis to further explore the relationship between the target. In addition, linkages between target proteins and cells were further identified using Gene Ontology (GO) and Pathways (KEGG Pathway). Finally, the repair effect of Guben Zenggu granule on MC3T3-E1 cells under continuous static pressure overload was verified through experiments, so as to accurately explain the pharmacodynamic mechanism of Traditional Chinese medicine.

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High water-soluble curcuminoids-rich extract regulates osteogenic differentiation of MC3T3-E1 cells: Involvement of Wnt/β-catenin and BMP signaling pathway

Cited by 10 publications

References 30 publications

The Effect of Ramania Leaves Extract Gel (Bouea macrophylla Griff) on the Number of Osteoblast (in vivo Study of Post Extraction in Wistar Rats (Rattus norvegicus))

The Effect of Ramania Leaves Extract Gel (Bouea macrophylla Griff) on the Number of Osteoblast (in vivo Study of Post Extraction in Wistar Rats (Rattus norvegicus))

Magnetic Hydroxyapatite Composite Nanoparticles for Augmented Differentiation of MC3T3-E1 Cells for Bone Tissue Engineering

Study on complications of osteoporosis based on network pharmacology

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