High-yield C11-oxidation of hydrocortisone by establishment of an efficient whole-cell system in Bacillus megaterium

König, Lisa; Hartz, Philip; Bernhardt, Rita; Hannemann, Frank

doi:10.1016/j.ymben.2019.06.005

Cited by 13 publications

(2 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Achieving regioselective oxidations with synthetic organic chemistry can be challenging so cytochrome P450 enzymes (P450s) are used instead. Several papers are describing the usage of B. megaterium as a host for whole-cell biotransformation with recombinant P450 enzymes, applied in pharmaceutical industry (Kleser et al 2012 ; Kiss et al 2015 ; König et al 2019 ; Richards et al 2020 ). For example, Richards et al ( 2020 ) explored different approaches to overcome limitations for whole-cell N-demethylation of noscapine with P450 BM3 (CYP102A1) mutant enzyme recombinantly produced in B. megaterium using xylose-inducible promoter P xylA .…”

Section: Molecular Tools Developed For Increased Production Of Recomb...mentioning

confidence: 99%

Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes

Vojnovic,

Aleksic,

Ilic-Tomic

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility. Key points • Bacillus and Streptomyces spp. as robust hosts for enzyme production. • Industrially relevant enzyme groups for production in alternative hosts highlighted. • Molecular biology techniques are enabling easier utilization of both hosts.

show abstract

Section: Molecular Tools Developed For Increased Production Of Recomb...mentioning

confidence: 99%

Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes

Vojnovic,

Aleksic,

Ilic-Tomic

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Bacillus megaterium is a rod-shaped, gram-positive prokaryotic bacterium, which has offered a green method to modify natural products ( König et al, 2019 ). Zhang and his coworkers found that Bacillus megaterium CGMCC 1.1741 could transform the ursolic acid (2) into three oxidation products.…”

Section: Whole Cell As a Catalystmentioning

confidence: 99%

Enzyme-catalyzed allylic oxidation reactions: A mini-review

et al. 2022

View full text Add to dashboard Cite

Chiral allylic oxidized products play an increasingly important role in the pharmaceutical, agrochemical, and pharmaceutical industries. Biocatalytic C–H oxyfunctionalization to synthesize allylic oxidized products has attracted great attention in recent years, with the ability to simplify synthetic approaches toward complex compounds. As a result, scientists have found some new enzymes and mutants through techniques of gene mining and enzyme-directed evolution in recent years. This review summarizes the recent developments in biocatalytic selective oxidation of olefins by different kinds of biocatalysts.

show abstract

Promoter engineering strategies for the overproduction of valuable metabolites in microbes

Jin

et al. 2019

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

High-yield C11-oxidation of hydrocortisone by establishment of an efficient whole-cell system in Bacillus megaterium

Cited by 13 publications

References 39 publications

Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes

Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes

Enzyme-catalyzed allylic oxidation reactions: A mini-review

Promoter engineering strategies for the overproduction of valuable metabolites in microbes

Contact Info

Product

Resources

About