High-yield clicking and dissociation of doxorubicin nanoclusters exhibiting differential cellular uptakes and imaging

Kim, Hye Sung; Yoon, Se Young; Son, Young Ju; Park, Yeonju; Jung, Young Mee; Yoo, Hyuk Sang

doi:10.1016/j.jconrel.2015.08.037

Cited by 8 publications

(13 citation statements)

References 42 publications

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“…We attribute these findings to the cleavage of the disulfide linkages and the Au-thiol linkages in the reducing condition, leading to the generation of the bare AuNPs. Thus, these results indicate that the polymer-multilayered shells decorating the AuNPs can be clearly peeled off where the reducing agents are abundant, such as in the cytosolic or endosomal compartments. − …”

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confidence: 87%

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Atom Transfer Radical Polymerization of Multishelled Cationic Corona for the Systemic Delivery of siRNA

et al. 2017

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We propose an effective siRNA delivery system by preparing poly(DAMA-HEMA)-multilayered gold nanoparticles using multiple surface-initiated atom transfer radical polymerization processes. The polymeric multilayer structure is characterized by transmission electron microscopy, matrix-associated laser desorption/ionization time-of-flight mass spectrometry, UV-vis spectroscopy, Fourier transform infrared spectroscopy, dynamic light scattering, and ζ-potential. The amount of siRNA electrostatically incorporated into the nanoparticle can be tuned by the number of polymeric shells, which in turn influences the cellular uptake and gene silencing effect. In a bioreductive environment, the interlayer disulfide bond breaks to release the siRNA from the degraded polymeric shells. Intravenously injected c-Myc siRNA-incorporated particles accumulate in the tumor site of a murine lung carcinoma model and significantly suppress the tumor growth. Therefore, the combination of a size-tunable AuNP core and an ATRP-functionalized shell offers control and versatility in the effective delivery of siRNA.

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confidence: 87%

“…Intravenous injection is still the most applicable form of administration, particularly for metastatic tumors. To overcome the limitation of unfavorable biodistribution in those cases, ligand-assisted active targeting − or size-dependent localization (clustering) , strategies will have to be adapted for the current delivery system.…”

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confidence: 99%

Atom Transfer Radical Polymerization of Multishelled Cationic Corona for the Systemic Delivery of siRNA

et al. 2017

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“…The fluorescence intensities of VE and VE-azide were 21.2 ± 11.6 and 132.5 ± 24.7, respectively, after reaction with alkyne-modified dyes. The total flux of VE-azide was 6.3-fold higher than that of VE, clearly indicating that VE amine groups were successfully modified with azide . After covalent conjugation of VEs onto MS via click chemistry, proteins in the various samples were analyzed by PAGE.…”

Section: Resultsmentioning

confidence: 99%

PLGA Microspheres Coated with Cancer Cell-Derived Vesicles for Improved Internalization into Antigen-Presenting Cells and Immune Stimulation

et al. 2019

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Microspheres (MS; 1−3 μm) with different degrees of surface roughness were prepared to assess the effects of surface topology on internalization into antigen-presenting cells (APCs; macrophages and dendritic cells). In this study, we demonstrated that the intracellular uptake of MS is readily enhanced by surface modification with nanoparticles or cancer cellderived vesicles (VE) to modulate their surface topology. MS coated with nanovesicles (MS-VE) with high surface roughness was more successfully and efficiently engulfed by APCs, compared with bare MS and those with low surface roughness. Incorporated MPLA within MS-VEs (M/MS-VE) triggered greatly elevated release of immune stimulating cytokines, interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α), from macrophages and dendritic cells, compared to free MPLA. Taken together, this MS-VE could serve as a platform system for the delivery of immune stimulators and antigens to APCs with negligible toxicity.

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“…Many types of MMP-2-responsive nanotheranostics emerge at the right moment. For instance, DOX/AuNPs/quantum dots (QDs) nanoclusters for fluorescence imaging (FI)-mediated anticancer treatment 114 , DOX-gelatin/epigallocatechin gallate/AuNPs nanoconjugates for FI-cooperated prostate cancer inhibition 115 , pH-responsive Au nanoclusters for synergistic near-infrared (NIR) FI and chemo-PDT of MMP-2 positive lung cancer 116 , MSN loaded with fluorescent and targeting peptides for FI-triggered tumor therapy 117 , dual-modal photoacoustic (PA)/NIR FI-enhanced photothermal therapy (PTT)/PDT for lung cancer 118 , and other theranostic nanosystems driven by additional optical principles including aggregation-induced emission (AIE) 119 and fluorescence resonance energy transfer (FRET) 120 . MSN- or 3D-printed biodegradable microswimmer-based magnetic nanoagents were designed for in vivo MMP-2-induced drug release and magnetic resonance imaging (MRI) 121 , 122 .…”

Section: Protease-responsive Nanodds For the Targeted Theranostics Of Malignancymentioning

confidence: 99%

“…DOX-encapsulated gold nanoclusters were presented for computed tomography (CT) imaging-visualized cancer chemotherapy 123 . The above MMP-2-encouraged representative achievements in cancer theranostics are summarized in Table 2 23 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 ...…”

Section: Protease-responsive Nanodds For the Targeted Theranostics Of Malignancymentioning

confidence: 99%

Protease-triggered bioresponsive drug delivery for the targeted theranostics of malignancy

Zhang

Guo

et al. 2021

Acta Pharmaceutica Sinica B

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Proteases have a fundamental role in maintaining physiological homeostasis, but their dysregulation results in severe activity imbalance and pathological conditions, including cancer onset, progression, invasion, and metastasis. This striking importance plus superior biological recognition and catalytic performance of proteases, combining with the excellent physicochemical characteristics of nanomaterials, results in enzyme-activated nano-drug delivery systems (nanoDDS) that perform theranostic functions in highly specific response to the tumor phenotype stimulus. In the tutorial review, the key advances of protease-responsive nanoDDS in the specific diagnosis and targeted treatment for malignancies are emphatically classified according to the effector biomolecule types, on the premise of summarizing the structure and function of each protease. Subsequently, the incomplete matching and recognition between enzyme and substrate, structural design complexity, volume production, and toxicological issues related to the nanocomposites are highlighted to clarify the direction of efforts in nanotheranostics. This will facilitate the promotion of nanotechnology in the management of malignant tumors.

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High-yield clicking and dissociation of doxorubicin nanoclusters exhibiting differential cellular uptakes and imaging

Cited by 8 publications

References 42 publications

Atom Transfer Radical Polymerization of Multishelled Cationic Corona for the Systemic Delivery of siRNA

Atom Transfer Radical Polymerization of Multishelled Cationic Corona for the Systemic Delivery of siRNA

PLGA Microspheres Coated with Cancer Cell-Derived Vesicles for Improved Internalization into Antigen-Presenting Cells and Immune Stimulation

Protease-triggered bioresponsive drug delivery for the targeted theranostics of malignancy

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