High yield expression and purification of recombinant human apolipoprotein A-II in Escherichia coli

Smith, Loren E.; Yang, Jun; Goodman, Leah; Huang, Xinqi; Huang, Rong; Dressman, James; Morris, Jamie; Silva, R. A. Gangani D.; Davidson, W. Sean; Cavigiolio, Giorgio

doi:10.1194/jlr.d028043

Cited by 13 publications

(17 citation statements)

References 44 publications

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“…A small fraction forms heterodimers with apolipoprotein D and E, and another minor fraction is present as a monomer [60]. The data of our and other groups of analyses using MALDI-TOF-MS indicated that there are three isoforms of circulating apoA2 homodimers in the blood stream of healthy subjects [61,62]. Moreover, we newly identified another two isoforms from the plasma of patients with PDAC [35].…”

Section: Possible Detection Of the Early Stage Of Pancreatic Cancer Andmentioning

confidence: 81%

Potential Usefulness of Apolipoprotein A2 Isoforms for Screening and Risk Stratification of Pancreatic Cancer

Honda¹,

Srivastava

2016

Biomark. Med.

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Given the low incidence of pancreatic cancer in the general population, screening of pancreatic cancer in the general population using invasive modalities is not feasible. Combination of invasive screening with noninvasive biomarkers for pancreatic cancer and its precancerous lesions has the potential to reduce mortality due to pancreatic cancer. In this review, we focus on biomarkers found in the blood that can indicate early-stage pancreatic cancer, and we discuss current strategies for screening for pancreatic cancer. We recently identified a unique alteration in apolipoprotein A2 isoforms in pancreatic cancer and its precancerous lesions, and we describe its clinical usefulness as a potential biomarker for the early detection and risk stratification of pancreatic cancer.

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Section: Possible Detection Of the Early Stage Of Pancreatic Cancer Andmentioning

confidence: 81%

Potential Usefulness of Apolipoprotein A2 Isoforms for Screening and Risk Stratification of Pancreatic Cancer

Honda¹,

Srivastava

2016

Biomark. Med.

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“…These advantages will be used for mass production and purification of rhPT2. The pTWIN1 vector which carries T7 promoter, which has been used to express large quantity of soluble recombinant protein (Studier et al 1991;Smith et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Codon Optimization and Chaperone Assisted Solubilization of Recombinant Human Prethrombin-2 Expressed in Escherichia coli

Silaban¹,

Maksum²,

Ghaffar³

et al. 2014

Microbiol Indones

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Prethrombin-2 (PT2) is a thrombin precursor that structurally has one glycosylation site and four disulphide bonds. As one of the fibrin glue components, thrombin is able to stick and to cover the wound. So, Thrombin can be potentially applied in replacing suture technic (Spotnitz and Prabhu 2005). Eventhough the fibrin glue has widespread applications, commercial fibrin glue has several disadvantages, i.e. allergic effect; pathogenic contamination, since the use of any plasma-derived product in the surgical setting carries a potential risk of viral transmission; and expensive protein therapeutics since must be imported (Spotnitz 2001; Enus et al. 2011). Generally, glycosylated protein therapeutics are produced from mammalian cells. Chinese Hamster Ovary (CHO) is the most commonly used mammalian

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“…These include chitin binding protein (CBP), maltose binding protein (MBP), and glutathione-S-transferase (GST) (27, 28). The poly (His) tag is also a widely-used protein tag that binds to metal matrices (29).…”

Section: Discussionmentioning

confidence: 99%

Purification of bacteriophage lambda repressor

Gao

Shearwin

Mack

et al. 2013

Protein Expression and Purification

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Bacteriophage lambda repressor controls the lysogeny/lytic growth switch after infection of E. coli by lambda phage. In order to study in detail the looping of DNA mediated by the protein, tag-free repressor and a loss-of-cooperativity mutant were expressed in E.coli and purified by (1) ammonium sulfate fractionation, (2) anion-exchange chromatography and (3) heparin affinity chromatography. This method employs more recently developed and readily available chromatography resins to produce highly pure protein in good yield. In tethered particle motion looping assays and atomic force microscopy “footprinting” assays, both the wild-type protein and a C-terminal His-tagged variant, purified using immobilized metal affinity chromatography, bound specifically to high affinity sites to mediate loop formation. In contrast the G147D loss-of-cooperativity mutant bound specifically but did not secure loops.

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High yield expression and purification of recombinant human apolipoprotein A-II in Escherichia coli

Cited by 13 publications

References 44 publications

Potential Usefulness of Apolipoprotein A2 Isoforms for Screening and Risk Stratification of Pancreatic Cancer

Potential Usefulness of Apolipoprotein A2 Isoforms for Screening and Risk Stratification of Pancreatic Cancer

Codon Optimization and Chaperone Assisted Solubilization of Recombinant Human Prethrombin-2 Expressed in Escherichia coli

Purification of bacteriophage lambda repressor

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