2017
DOI: 10.1099/jmm.0.000439
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High-yield extraction of Escherichia coli RNA from human whole blood

Abstract: For most applications, lysis of the human blood cells is not required. However, co-purified human RNA may interfere with some downstream processes such as RNA sequencing. In this case, blood cell lysis with Triton X-100 is desirable.

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Cited by 6 publications
(2 citation statements)
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“…Due to the increasing demand for rapid, culture-independent molecular detection methods, a plethora of multiplex PCR detection systems for E. coli and total coliform bacteria have been developed during the last decade [49,50]. Recently, Krapft et al revealed a clear correlation between viable cell counts and real-time quantitative PCR (qPCR) data [51].…”
Section: Discussionmentioning
confidence: 99%
“…Due to the increasing demand for rapid, culture-independent molecular detection methods, a plethora of multiplex PCR detection systems for E. coli and total coliform bacteria have been developed during the last decade [49,50]. Recently, Krapft et al revealed a clear correlation between viable cell counts and real-time quantitative PCR (qPCR) data [51].…”
Section: Discussionmentioning
confidence: 99%
“…The supplementary materials include Additional File 1 (Tables) and Additional File 2 (Figures). The references [66][67][68][69][70] are cited in the supplementary materials: Additional File 1 (Tables).…”
Section: Abbreviationsmentioning
confidence: 99%