High Yield of Butanol Production in Repeated Batch Culture Fermentation by Clostridium Acetobutylicum YM1

Abstract

Butanol is the most promising alternative liquid biofuel that can substitute gasoline due to its properties which is more similar to gasoline than ethanol. In this study, butanol was produced by batch culture and repeated batch culture in acetone-butanol-ethanol fermentation (ABE) using a local strain of Clostridium acetobutylicum YM1. In batch culture using tryptone-yeast extract-acetate medium (TYA) containing 30 g/L glucose, the maximum butanol concentration was 3.78 g/L with a maximum butanol productivity of 0.08 g/L.h and the butanol yield was 0.15 g/g. Effect of some factors on the butanol production during repeated batch fermentation including drain and fill volume of medium and drain and fill time were investigated. The results of repeated batch fermentation showed that 50% (v/v) of drain and fill volume at 48 h maximized the production of butanol, productivity and yield. The highest butanol concentration was 5.12 g/L in the first cycle of repeated batch with a butanol productivity of 0.11 g/L.h and a butanol yield of 0.44 g/g. Repeated batch fermentation using free cells of Clostridium acetobutylicum YM1 eliminated the lag phase and then improved the productivity of butanol and total ABE. This study showed that repeated batch fermentation improved the efficiency of butanol production over batch culture fermentation by Clostridium acetobutylicum YM1.

Bibliometric Analysis on Biobutanol Production Research Trends from 2010-2022 Using Scopus Database

“…Biobutanol has good characteristics such as less corrosive, low volatility, high heat value, high viscosity and high hydrophobicity which are comparable to gasoline, demonstrating the benefit of selecting biobutanol as the next generation of biofuels (Jiang et al 2018;Jiménez-Bonilla & Wang 2018;Khalifa et al 2018;Peabody & Kao 2016;Salleh et al 2019). This clearly depicts the fundamental reasons behind the choice of utilizing biobutanol as an alternative source of renewable energy.…”

Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia

“…For example, with the help of membrane cell-recycle bioreactor, a high cell density continuous ABE fermentation of C. acetobutylicum BKM19 was carried out to produce butanol and ABE with the volumetric productivities of 10.7 and 21.1 g/L/h, the production of 11.9 and 23.5 g/L, and the yields of 0.17 and 0.34 g/g, respectively, under the optimal operational condition [195]. Generally, a productivity of > 10 g/L/h, titer of > 10 g/L butanol, and yield up to 0.44 g/g could be achieved from a high-celldensity fermentation, a great success in ABE fermentation by Clostridium [196,197].…”

Novel Batch and Repeated-Batch Butanol Fermentation from Sweet Sorghum Stem Juice by Co-Culture of Arthrobacter and Immobilized Clostridium in Scaled-Up Bioreactors

“…Consequently, repeated-batch fermentation is considered superior to the conventional batch fermentation because it addresses some of these weaknesses. Additionally, repeated-batch fermentations require less time, reuse microbial cells in subsequent fermentation runs and have high cell concentrations during fermentation [13,14].…”