High yield production of monomer-free chitosan oligosaccharides by pepsin catalyzed hydrolysis of a high deacetylation degree chitosan

Roncal, Tomás; Oviedo, Alberto; Armentia, Iratxe López de; Fernández, L.; Villarán, María Carmen

doi:10.1016/j.carres.2007.08.023

Cited by 86 publications

(83 citation statements)

References 22 publications

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“…The total of reducing sugars was measured by spectrophotometric analysis by the Schales and Schales method [26]. The Schales reagent was prepared as follows: 0.50 g of potassium ferricyanide was mixed into 1 L of potassium carbonate solution (0.5 M) and then was stored in a dark bottle.…”

Section: Characterisations Of Hydrolyzed Chitosan Productmentioning

confidence: 99%

See 1 more Smart Citation

Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition

Rokhati

Susanto

Haryani

et al. 2017

Period. Polytech. Chem. Eng.

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Section: Characterisations Of Hydrolyzed Chitosan Productmentioning

confidence: 99%

“…The Schales reagent was added to the chitosan sample solution and then heated, causing a redox reaction between the Schales reagent and the aldehyde group so that the solution color turned from yellow/red to transparent. The scanning range was recorded between 350 and 500 nm [26,27].…”

Section: Characterisations Of Hydrolyzed Chitosan Productmentioning

confidence: 99%

Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition

Rokhati

Susanto

Haryani

et al. 2017

Period. Polytech. Chem. Eng.

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“…Pepsin Digestion of Chitosan-As an alternative way to examine the effect of particle size, we took advantage of the ability of pepsin to partially digest chitosan (37). Following the procedure outlined in Fig.…”

Section: Chitosan Stimulates the Inflammasome And Chitin Does Not-mentioning

confidence: 99%

Chitosan but Not Chitin Activates the Inflammasome by a Mechanism Dependent upon Phagocytosis

Bueter

Lee

Rathinam

et al. 2011

Journal of Biological Chemistry

173

126

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Chitin is an abundant polysaccharide found in fungal cell walls, crustacean shells, and insect exoskeletons. The immunological properties of both chitin and its deacetylated derivative chitosan are of relevance because of frequent natural exposure and their use in medical applications. Depending on the preparation studied and the end point measured, these compounds have been reported to induce allergic responses, inflammatory responses, or no response at all. We prepared highly purified chitosan and chitin and examined the capacity of these glycans to stimulate murine macrophages to release the inflammasome-associated cytokine IL-1β. We found that although chitosan was a potent NLRP3 inflammasome activator, acetylation of the chitosan to chitin resulted in a near total loss of activity. The size of the chitosan particles played an important role, with small particles eliciting the greatest activity. An inverse relationship between size and stimulatory activity was demonstrated using chitosan passed through size exclusion filters as well as with chitosan-coated beads of defined size. Partial digestion of chitosan with pepsin resulted in a larger fraction of small phagocytosable particles and more potent inflammasome activity. Inhibition of phagocytosis with cytochalasin D abolished the IL-1β stimulatory activity of chitosan, offering an explanation for why the largest particles were nearly devoid of activity. Thus, the deacetylated polysaccharide chitosan potently activates the NLRP3 inflammasome in a phagocytosis-dependent manner. In contrast, chitin is relatively inert.

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“…Different acid used for chitosanolysis are HCl (Kasaai et al, 2013), H 2 SO 4 (Zamani and Taherzadeh, 2010), NaNO 2 (Hussain et al, 2012), formic acid (Yamaguchi et al,1982), lactic acid (Il'ina and Varlamov 2004) etc. Enzymatic hydrolysis of chitin and chitosan can be done by chitinase (Yoon, 2005;Sorbotten et al, 2005), chitosanase (Varum et al, 1996;Kuroiwa et al, 2009), cellulase (Xie et al, 2009), hemicellulase (Qin et al,2003), pectinase (Kittur et al,2005), papain (Vishu Kumar et al, 2004) and pepsin (Roncal et al, 2007) and physical methods such as ultrasonication , microwave (Xing et al, 2005), gamma rays (Yoksan et al, 2004) etc. can be used for COS production.…”

Section: Introductionmentioning

confidence: 99%

Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

Varun

Senani

Kumar

et al. 2017

IJAR

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An experiment was carried out to utilize the crab shell waste for the production of chitin by chemical extraction method. The obtained chitin was processed to make the more useful compound chitosan by deacetylation of chitin using strong NaOH solution at high temperature. Yield of chitin and chitosan from crab shell waste was found to be 12.2 % and 10.54 %, respectively. Chitosan was further used to produce chito-oligosaccharides (COS) by acid hydrolysis using 0.5 N H 2 SO 4 , 1N H 2 SO 4 and 7N HCl at hourly intervals upto 6 hours and the average yield was 0.55, 0.84 and 1.93 mg/ml, respectively. Chitin and chitosan were characterized by Fourier Transform Infrared spectroscopy (FT-IR) technique which depicts different band representing various bonds present in the molecule and which were in agreement with the bands of standard chitin and chitosan. Further, COS were formed after acid hydrolysis of chitosan, which was characterized by Thin Layer Chromatography (TLC) using glucosamine as a standard.

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High yield production of monomer-free chitosan oligosaccharides by pepsin catalyzed hydrolysis of a high deacetylation degree chitosan

Cited by 86 publications

References 22 publications

Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition

Enhanced Enzymatic Hydrolysis of Chitosan by Surfactant Addition

Chitosan but Not Chitin Activates the Inflammasome by a Mechanism Dependent upon Phagocytosis

Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

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