Higher Leukocyte 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and Lower Plasma Ascorbate in Aging Humans?

Siomek, Agnieszka; Gackowski, Daniel; Różalski, Rafał; Dziaman, Tomasz; Szpila, Anna; Guz, Jolanta; Oliński, Ryszard

doi:10.1089/ars.2007.9.143

Cited by 75 publications

(63 citation statements)

References 36 publications

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“…Urine samples were frozen at −85°C and stored for no more than 1 month. The precise description of the method that was used for quantitative assessment was given previously (10). The levels of creatinine has been determined colorimetrically by the Jaffe reaction.…”

Section: )mentioning

confidence: 99%

“…The blood with cells was carefully applied on top of Histopaque 1119 solution (Sigma), and leukocytes were isolated by centrifugation according to the procedure described by the manufacturer. Quantification of 8-oxodG in DNA isolates was described previously (10). Since it was recently shown that the extent of artifactual formation of 8-oxodGua is inversely dependent on the amount of extracted DNA, we used only those samples for which the quantity of DNA extracted was in the range 100 to 300 μg.…”

Section: )mentioning

confidence: 99%

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Selenium Supplementation Reduced Oxidative DNA Damage in Adnexectomized BRCA1 Mutations Carriers

Dziaman

Huzarski

Gackowski

et al. 2009

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Some experimental evidence suggests that BRCA1 plays a role in repair of oxidative DNA damage. Selenium has anticancer properties that are linked with protection against oxidative stress. To assess whether supplementation of BRCA1 mutation carriers with selenium have a beneficial effect concerning oxidative stress/DNA damage in the present double-blinded placebo control study, we determined 8-oxodG level in cellular DNA and urinary excretion of 8-oxodG and 8-oxoGua in the mutation carriers. We found that 8-oxodG level in leukocytes DNA is significantly higher in BRCA1 mutation carriers. In the distinct subpopulation of BRCA1 mutation carriers without symptoms of cancer who underwent adnexectomy and were supplemented with selenium, the level of 8-oxodG in DNA decreased significantly in comparison with the subgroup without supplementation. Simultaneously in the same group, an increase of urinary 8-oxoGua, the product of base excision repair (hOGG1 glycosylase), was observed. Therefore, it is likely that the selenium supplementation of the patients is responsible for the increase of BER enzymes activities, which in turn may result in reduction of oxidative DNA damage. Importantly, in a double-blinded placebo control prospective study, it was shown that in the same patient groups, reduction in cancer incidents was observed. Altogether, these results suggest that BRCA1 deficiency contributes to 8-oxodG accumulation in cellular DNA, which in turn may be a factor responsible for cancer development in women with mutations, and that the risk to developed breast cancer in BRCA1 mutation carriers may be reduced in selenium-supplemented patients who underwent adnexectomy. (Cancer Epidemiol Biomarkers Prev 2009;18(11):2923-8)

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confidence: 99%

Section: )mentioning

confidence: 99%

Selenium Supplementation Reduced Oxidative DNA Damage in Adnexectomized BRCA1 Mutations Carriers

Dziaman

Huzarski

Gackowski

et al. 2009

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…5 Determination of 8-oxodG by the mean of HPLC/EC technique was described previously. 5,6 Urine analysis Urine sample preparation, HPLC purification and GC/MS analysis were conducted as described earlier. 5 …”

Section: Dna Isolation and 8-oxodg Determination In Dna Isolatesmentioning

confidence: 99%

“…5,6 Urine analysis Urine sample preparation, HPLC purification and GC/MS analysis were conducted as described earlier. 5 …”

Section: Dna Isolation and 8-oxodg Determination In Dna Isolatesmentioning

confidence: 99%

Small field radiotherapy of head and neck cancer patients is responsible for oxidatively damaged DNA/oxidative stress on the level of a whole organism

Roszkowski¹,

Gackowski

Różalski

et al. 2008

Intl Journal of Cancer

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It is possible that oxidatively damaged DNA which arises as a result of radiotherapy may be involved in the therapeutic effect of the ionizing radiation and in the side effects. Therefore, for the first time, the broad spectrum of oxidatively damaged DNA biomarkers: urinary excretion of 8-oxodG (8-oxo-7,8-dihydro-2 0 -deoxyguanosine), as well as the level of oxidatively damaged DNA in leukocytes, was analyzed in head and neck cancer patients (n 5 27) undergoing fractionated radiotherapy using methodologies which involve HPLC (high-performance liquid chromatography) prepurification followed by gas chromatography with isotope dilution mass spectrometry detection and HPLC/EC. Of all the analyzed parameters in the majority of patients, only urinary excretion of the modified nucleoside significantly increased over the initial level in the samples collected 24 hr after the last fraction. However, for the distinct subpopulation of 10 patients, a significant increase in the level of 8-oxodG in cellular DNA and a simultaneous drop in urinary 8-oxoGua (the repair product of oxidative DNA damage) were detected after completion of the therapy. Because 8-oxoGua is a repair product of the DNA damage, there is a possibility that, at least in the case of some patients with the lowest activity of OGG1 (8-oxo-7,8-dihydroguanine glycosylase), the combination of lower OGG1 repair efficacy and irradiation was associated with increased background level of 8-oxoGua in cellular DNA. Apparently reduced DNA repair is unable to cope with the radiation-induced, and the extra amount of 8-oxoGua leading to an increase of potentially mutagenic/carcinogenic lesions.

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“…The concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the liver were measured by the modified Foksiński method [22] using high--performance liquid chromatography -ultraviolet and electrochemical detectors (HPLC/UV/ECD). DNA hydrolysates were analyzed by isocratic elution chromatography using 25 mM sodium acetate, 12.5 mM citrate (pH 5.0)-methanol (88:12, v/v).…”

Section: Liver Tissue Sampling and Testingmentioning

confidence: 99%

Alpha-Tocopherol May Protect Hepatocytes Against Oxidative Damage Induced by Endurance Training in Growing Organisms

et al. 2016

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Higher Leukocyte 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine and Lower Plasma Ascorbate in Aging Humans?

Cited by 75 publications

References 36 publications

Selenium Supplementation Reduced Oxidative DNA Damage in Adnexectomized BRCA1 Mutations Carriers

Selenium Supplementation Reduced Oxidative DNA Damage in Adnexectomized BRCA1 Mutations Carriers

Small field radiotherapy of head and neck cancer patients is responsible for oxidatively damaged DNA/oxidative stress on the level of a whole organism

Alpha-Tocopherol May Protect Hepatocytes Against Oxidative Damage Induced by Endurance Training in Growing Organisms

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