Higher NORs-expression in lymphocyte of trisomy 21 babies/children: In vivo evaluation

İmamoğlu, Nalan; Demirtaş, Halil; Dönmez-Altuntaş, Hamiyet; Ilten, Ali

doi:10.1016/j.micron.2005.05.002

Cited by 15 publications

(18 citation statements)

References 15 publications

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“…Imamoglu et al (23) reported that in vivo lymphocytes of 54 babies/children (0-8 years old) with DS have more mean AgNOR surface (NORs proteins) than that of healthy controls. AgNOR staining is related however with NOR proteins and its level cannot be taken a direct indicator of the RNA/rRNA content of the cells.…”

Section: Discussionmentioning

confidence: 99%

Flow cytometric comparison of RNA content in peripheral blood mononuclear cells of down syndrome patients and control individuals

Hamurcu

Demirtaş

Kumandaş

2005

Cytometry Part B Clinical

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Objective: Trisomy 21 or Down syndrome (DS) is the most common genetic cause of mental retardation associated with the immunologic and other known defects. Extra chromosome 21 of DS patients contains an average of 40 extra copies of rRNA genes and the in vivo regulation of these genes' activity is not known. Because over 80% of total cellular RNA is rRNA, the measurement of total cellular RNA provides information on rRNA content. The aim of this work was to determine whether or not the additional chromosome 21 causes any increase in total cellular RNA content in mononuclear cells from peripheral blood (PBMNCs) of these patients and whether or not this content is modified with age.Method: PBMNCs of 48 patients with DS and 48 healthy controls were studied. RNA content of isolated PBMNCs was evaluated by flow cytometric measurements.Results: Average RNA content of younger DS patients' cells was significantly higher than that of healthy controls (P = 0.003). Furthermore, the RNA content decreased significantly with increasing age of DS patients (r = -0.377, P = 0.008) in the range of 0-26 year old, whereas no significant relationship was found between age and PBMNCs' RNA content of healthy controls in the same range of ages.Conclusion: RNA content of PBMNCs from DS patients decreases rapidly with age. This is the first work on the age-dependent decrease of the RNA content in PBMNCs of DS patients. q 2005 International Society for Analytical Cytology

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Section: Discussionmentioning

confidence: 99%

Flow cytometric comparison of RNA content in peripheral blood mononuclear cells of down syndrome patients and control individuals

Hamurcu

Demirtaş

Kumandaş

2005

Cytometry Part B Clinical

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“…AgNORs have been shown to be of interest in a variety of different organs and disease states such as lymphoma, genetic diseases [3], and thyroid disorders [4,5]. In the adult population, thyroid nodules are common and are increasingly detected by ultrasound examination or other scanning techniques.…”

Section: Introductionmentioning

confidence: 99%

The Evaluation of Argyrophilic Nucleolar Organizing Region Proteins in Fine-Needle Aspiration Samples of Thyroid

et al. 2011

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Argyrophilic nucleolar organizing region associated proteins (AgNORs) have been shown to be of interest in a variety of different diseases including thyroid disorders. Our aim was to distinguish benign thyroid lesions from papillary thyroid carcinoma (PTC) via AgNOR count and with a new approach, via AgNOR surface area/total nuclear surface area (NORa/TNa) proportions in the nuclei on fine-needle aspiration (FNA) materials. Thirty patients (eight men and 22 women) whose FNA was compatible with benign lesion and 26 patients (eight men and 18 women) whose FNA was compatible with PTC were included in the study. Fine-needle aspiration materials were stained for AgNOR detection according to a specific protocol. One hundred nuclei per individual have been evaluated, and AgNOR number and NORa/TNa proportions of individual cells were measured and calculated by using a computer program. Patients with PTC had significantly (p<0.001) higher AgNOR count (4.6 ± 1.2%) than in the patients with benign lesions (2.0 ± 0.5%). Additionally, patients with PTC had significantly (p<0.001) higher NORa/TNa (13.4 ± 2.4) than in the patients with benign lesion (5.7 ± 1.0). Modified method of AgNOR staining is an easy and reliable method for evaluating proliferation activity of cells in malignant and benign thyroid lesions and it may contribute to routine cytopathology in inconclusive situations.

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“…AgNOR image analysis studies show that mitogenstimulated (5-7) and nonstimulated (in vivo) lymphocytes (8) of DS babies/children have more NORs expression than healthy controls. Furthermore, NOR bearing chromosome number of metaphase lymphocytes from babies/children with DS were higher compared to those of the controls (9).…”

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confidence: 99%

Age‐dependent decreases in mitogen‐stimulation level and RNA content in peripheral blood mononuclear cells of down syndrome patients

Hamurcu

Demirtaş

Patıroğlu

et al. 2006

Cytometry Part B Clinical

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Objective: The aim of the present study was to determine whether or not the phytohemagglutinin (PHA)-activated proliferation and average RNA content in peripheral blood mononuclear cells (PBMC) of Down syndrome (DS) patients change with age.Method: Stimulated portion of PBMC and total RNA levels in these cells after 72 h of PHA stimulation from 38 DS patients were compared with 28 age-matched healthy controls using flow cytometric measurement.Results: Decreased ratio of PBMC from DS patients undergoes mitogenic stimulation with age (r = À0.84, P = 0.000). This decrease is not observed in the cells of control individuals (r = 0.03, P = 0.869). Stimulated PBMC in infants with DS have higher level of RNA contents compared to controls (Z = 2.227, P = 0.026). While RNA content in mitogen-stimulated PBMC of DS decreased progressively and significantly with age (r = À0.70, P = 0.000), no significant age-related change in RNA content was found among the cells of healthy individuals in the range of 0-27 year old (r = 0.275, P = 0.157, P > 0.05).Conclusion: Age-dependent decreases in mitogen-activated proliferation ratio and average RNA content of PBMC from DS patients appear as regular events. These results may contribute to the explanation of the immune deficiency seen in DS patients since the PHA-stimulated cells are principally T-lymphocytes. This is the first report on the decrease in PHA-stimulated proliferation ratio (stimulability) and RNA level in PBMC of DS patients in relation to age. q 2006 Clinical Cytometry Society

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Higher NORs-expression in lymphocyte of trisomy 21 babies/children: In vivo evaluation

Cited by 15 publications

References 15 publications

Flow cytometric comparison of RNA content in peripheral blood mononuclear cells of down syndrome patients and control individuals

Flow cytometric comparison of RNA content in peripheral blood mononuclear cells of down syndrome patients and control individuals

The Evaluation of Argyrophilic Nucleolar Organizing Region Proteins in Fine-Needle Aspiration Samples of Thyroid

Age‐dependent decreases in mitogen‐stimulation level and RNA content in peripheral blood mononuclear cells of down syndrome patients

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