Higher‐Order Structures Based on Molecular Interactions for the Formation of Natural and Artificial Biomaterials

Künzle, Matthias; Lach, Marcel; Budiarta, Made; Beck, Tobias

doi:10.1002/cbic.201800824

Cited by 6 publications

(4 citation statements)

References 32 publications

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“…Tissue engineering has been used in various clinical applications, for example, vascular scaffolds [ 2 ], skin repair [ 3 ], bone tissue reconstruction [ 4 ], cartilage replacement [ 5 ], and cardiac muscle tissue regeneration [ 6 ]. In brief, the biomaterials that are applied in tissue regeneration approaches can be classified into natural and artificial types [ 7 ]. However, there are a number of factors that should be taken into consideration, such as biocompatibility and biodegradability [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Neural Differentiation Potential of Mesenchymal Stem Cells Enhanced by Biocompatible Chitosan-Gold Nanocomposites

Hung

Yang

Chang

et al. 2022

Cells

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Chitosan (Chi) is a natural polymer that has been demonstrated to have potential as a promoter of neural regeneration. In this study, Chi was prepared with various amounts (25, 50, and 100 ppm) of gold (Au) nanoparticles for use in in vitro and in vivo assessments. Each as-prepared material was first characterized by UV-visible spectroscopy (UV-Vis), Fourier-transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), scanning electron microscopy (SEM), and Dynamic Light Scattering (DLS). Through the in vitro experiments, Chi combined with 50 ppm of Au nanoparticles demonstrated better biocompatibility. The platelet activation, monocyte conversion, and intracellular ROS generation was remarkably decreased by Chi–Au 50 pm treatment. Furthermore, Chi–Au 50 ppm could facilitate colony formation and strengthen matrix metalloproteinase (MMP) activation in mesenchymal stem cells (MSCs). The lower expression of CD44 in Chi–Au 50 ppm treatment demonstrated that the nanocomposites could enhance the MSCs undergoing differentiation. Chi–Au 50 ppm was discovered to significantly induce the expression of GFAP, β-Tubulin, and nestin protein in MSCs for neural differentiation, which was verified by real-time PCR analysis and immunostaining assays. Additionally, a rat model involving subcutaneous implantation was used to evaluate the superior anti-inflammatory and endothelialization abilities of a Chi–Au 50 ppm treatment. Capsule formation and collagen deposition were decreased. The CD86 expression (M1 macrophage polarization) and leukocyte filtration (CD45) were remarkably reduced as well. In summary, a Chi polymer combined with 50 ppm of Au nanoparticles was proven to enhance the neural differentiation of MSCs and showed potential as a biosafe nanomaterial for neural tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Neural Differentiation Potential of Mesenchymal Stem Cells Enhanced by Biocompatible Chitosan-Gold Nanocomposites

Hung

Yang

Chang

et al. 2022

Cells

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“…This allows their use as versatile platforms for medical-and nanotechnological-applications to be explored. While different approaches for such exterior decoration have been devised and summarised in several reviews, 56,57 here we update and highlight recent efforts to functionalize protein cage surfaces with a particular focus on studies aiming at macromolecular display and lattice formation.…”

Section: Connectability Of Cage Exteriormentioning

confidence: 99%

Connectability of protein cages

2020

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“…It is important to note that specific interactions between the container subunits afford the self‐assembly into the fully assembled container. These interactions have evolved to form stable containers at neutral conditions and are usually weak, non‐covalent forces composed of hydrogen bonding, electrostatic interactions and van der Waals interactions . For reversible disassembly of the container, the interactions forces between the subunits must be temporarily neutralized.…”

Section: Protein Containers For Multi‐component Biohybrid Structuresmentioning

confidence: 99%

Multi‐Component Self‐Assembly of Proteins and Inorganic Particles: From Discrete Structures to Biomimetic Materials

Künzle

Lach

Beck

2019

Israel Journal of Chemistry

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Due to their defined structure, proteins are suitable building blocks for the bottom‐up construction of multi‐component materials. Especially protein containers, with their inherent cavity, can be used to encapsulate synthetic components such as inorganic nanoparticles. This way, multi‐component discrete structures can be assembled. With recent advances in computational protein design, novel protein containers were successfully created, with a high potential for application in biomedicine and materials research. Moreover, engineered protein containers offer a unique building block for the self‐assembly of three‐dimensional materials. They combine the molecular precision of proteins with nanoscale dimensions. Designed interactions lead to novel protein scaffolds. In addition, nanoparticles encapsulated inside the container cavity introduce orthogonal functionality, important for the realization of nanostructured biomimetic materials with emergent properties.

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Higher‐Order Structures Based on Molecular Interactions for the Formation of Natural and Artificial Biomaterials

Cited by 6 publications

References 32 publications

Neural Differentiation Potential of Mesenchymal Stem Cells Enhanced by Biocompatible Chitosan-Gold Nanocomposites

Neural Differentiation Potential of Mesenchymal Stem Cells Enhanced by Biocompatible Chitosan-Gold Nanocomposites

Connectability of protein cages

Multi‐Component Self‐Assembly of Proteins and Inorganic Particles: From Discrete Structures to Biomimetic Materials

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