Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression

Huang, Yunte; Blakeley, Stephen D.; McALEESE, Sybil M.; Fothergill‐Gilmore, Linda A.; Dennis, David T.

doi:10.1007/bf00021818

Cited by 19 publications

(27 citation statements)

References 45 publications

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“…7) Phosphoglycerate mutase: The Northern band population (Figure 2(b)) obtained using the cDNA probe for the mRNA encoding co-factor independent PGlycM showed that they were about 1.8 kb molecular weight similar to that of other higher plants [44] [45]. The mRNA encoding the enzyme was silenced in control peanut, incompletely silenced in KN and NPKS-treated peanuts, but not silenced in NPPK-treated peanut.…”

Section: Demonstration Of Silencing Of Mrnas Encoding the Non-exergonmentioning

confidence: 97%

Enhancement of the Essential Amino Acid Composition of Food Crop Proteins through Biotechnology

Osuji

Duffus

Johnson

et al. 2015

AJPS

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Lack of essential amino acids (EAA) in the diet of at-risk populations could beget a state of food insecurity. Plant proteins are deficient in some essential amino acids. Animals obtain EAA from plant sources. Simple biotechnologies are being developed for improving the EAA composition of crop proteins. The aim was to integrate-discriminate glycolysis and citric-glyoxylic acid cycles to optimize biosynthesis of EAA in food crops. Permutation of diverse metabolic pathways at the mRNA level by glutamate dehydrogenase (GDH)-synthesized RNA is a common biotechnology for doubling the nutritious compositions of plants. Peanuts were planted in plots and treated with mineral salts mixed according to stoichiometric ratios. Protein-bounded and free amino acids of mature peanut seeds were determined by HPLC. GDH-synthesized RNA probes homologous to the mRNAs encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate mutase (PGlycM), phosphoenolpyruvate carboxylase (PEPCase), enolase, malate dehydrogenase (MDH), isocitrate lyase (ICL), and malate synthase (MS) were prepared from peanut seeds using restriction fragment double differential display PCR method. Northern assays of peanut total RNA showed that the mRNAs encoding PGlycM, PEPCase, MDH, and MS shared extensive sequence homologies that produced a dense network of cross-talks, resulting to co-differential silencing of the mRNAs thereby permuting glycolysis, citric-glyoxylic acid cycles. There were 42 permutations in the NPPKtreated, 105 in control, 420 in KN-, and NPKS-treated peanuts. Because of permutations involving the mRNAs encoding ICL and MS, wherever the abundances of these mRNAs were high (control, and NPPK-treated peanuts) the concentrations of the α-ketoglutarate group of total glutamate, glutamine, arginine, proline, and histidine were minimized (<7.0 mg/g) but the concentrations of the oxaloacetate group of total aspartate, lysine, methionine, threonine, and isoleucine were maxi-* Corresponding author. G. O. Osuji et al.3092 mized (>28.0 mg/g). The integration of glycolysis, citric and glyoxylic acid cycles increased the quality and doubled the concentrations of the protein-bounded EAA composition of NPPK-treated (33.37 mg/g) compared with the control peanut (15.66 mg/g). The commanding biotechnology was the stoichiometric mineral salts-based induction of GDH to synthesize the RNAs that integrated glycolysis, citric-glyoxylic acid cycles to one functional unit.

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Section: Demonstration Of Silencing Of Mrnas Encoding the Non-exergonmentioning

confidence: 97%

Enhancement of the Essential Amino Acid Composition of Food Crop Proteins through Biotechnology

Osuji

Duffus

Johnson

et al. 2015

AJPS

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“…We now know that more than one structure can provide the same function and that analogy rather than homology best describes the situation for a number of enzymes. Such is the case for the mutases of glycolysis (Britton et al 1971;Grana et al 1992;Huang et al 1993;Ossa et al 1994).…”

Section: Introductionmentioning

confidence: 93%

“…The two mutases show no detectable sequence similarity (Ossa et al 1994). The PGAM-i from plants is similar to PGAM-i in Neurospora, the only non-plant PGAM-i for which there is any sequence (Huang et al 1993). Grana et al (1992) suggested that PGAM-i in maize showed sequence similarity to the alkaline phosphatase super-family, but this is controversial (Huang et al 1993).…”

Section: Introductionmentioning

confidence: 95%

“…Only recently have the gene and protein sequences of PGAM-i been available for comparison with PGAM (Grana et al , 1993Huang et al 1993;Ossa et al 1994). The two mutases show no detectable sequence similarity (Ossa et al 1994).…”

Section: Introductionmentioning

confidence: 96%

“…The better studied of the twocofactor-dependent phosphoglycerate mutase, or PGAM -occurs in vertebrates (Fothergill-Gilmore and Watson 1989). The gene sequences for PGAMs described so far show strong conservation in the evolution of this ancient enzyme (Huang et al 1993). Cofactor-independent phosphoglycerate mutase (PGAM-i) is the only mutase found in plants, and far less is known about this enzyme because of its instability and difficulties in its purification.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lily cofactor-independent phosphoglycerate mutase: purification, partial sequencing, and immunolocalization

Wang

Walling

Jauh

et al. 1996

Planta

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A cofactor-independent phosphoglycerate mutase (PGAM-i) was isolated to homogeneity from monocotyledonous Lilium longiflorum Thunb. Two-dimensional (2D) polyacrylamide gel electrophoresis resolved three PGAM-i forms. This enzyme was originally identified by cross-reactivity to antibodies affinity-purified from 2D gels using human vitronectin (VN). Antibody produced against a denatured protein spot from a 2D gel did not recognize VN protein, but partial protein and DNA sequencing showed similarity of the former protein to maize PGAM-i. Immunoblots from roots, styles, leaves, and anthers showed the presence of PGAM-i in all tissues examined; it was isolated predominantly from the soluble cell fraction, with some present in the insoluble cell fraction. Immunoelectron microscopy demonstrated its localization in the cytoplasm and plastids in root cells near the apical meristem. In addition, immunogold labeling detected signals from the nucleus. The immunohistochemical localization of the enzyme in the nucleus, as well as in the cytosol and plastids, indicates that lily PGAM-i might have multiple functions in the cell.

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Proteins associated with heat-induced leaf senescence in creeping bentgrass as affected by foliar application of nitrogen, cytokinins, and an ethylene inhibitor

Jespersen

Huang

2015

Proteomics

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Heat stress causes premature leaf senescence in cool-season grass species. The objective of this study was to identify proteins regulated by nitrogen, cytokinins, and ethylene inhibitor in relation to heat-induced leaf senescence in creeping bentgrass (Agrostis stolonifera). Plants (cv. Penncross) were foliar sprayed with 18 mM carbonyldiamide (N source), 25 μM aminoethoxyvinylglycine (AVG, ethylene inhibitor), 25 μM zeatin riboside (ZR, cytokinin), or a water control, and then exposed to 20/15°C (day/night) or 35/30°C (heat stress) in growth chambers. All treatments suppressed heat-induced leaf senescence, as shown by higher turf quality and chlorophyll content, and lower electrolyte leakage in treated plants compared to the untreated control. A total of 49 proteins were responsive to N, AVG, or ZR under heat stress. The abundance of proteins in photosynthesis increased, with ribulose-1,5-bisphosphate carboxylase/oxygenase affected by all three treatments, chlorophyll a/b-binding protein by AVG and N or Rubisco activase by AVG. Proteins for amino acid metabolism were upregulated, including alanine aminotransferase by three treatments and ferredoxin-dependent glutamate synthase by AVG and N. Upregulated proteins also included catalase by AVG and N and heat shock protein by ZR. Exogenous applications of AVG, ZR, or N downregulated proteins in respiration (enolase, glyceraldehyde 3-phosphate dehydrogenase, and succinate dehygrogenase) under heat stress. Alleviation of heat-induced senescence by N, AVG, or ZR was associated with enhanced protein abundance in photosynthesis and amino acid metabolism and stress defense systems (heat shock protection and antioxidants), as well as suppression of those imparting respiration metabolism.

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Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression

Cited by 19 publications

References 45 publications

Enhancement of the Essential Amino Acid Composition of Food Crop Proteins through Biotechnology

Enhancement of the Essential Amino Acid Composition of Food Crop Proteins through Biotechnology

Lily cofactor-independent phosphoglycerate mutase: purification, partial sequencing, and immunolocalization

Proteins associated with heat-induced leaf senescence in creeping bentgrass as affected by foliar application of nitrogen, cytokinins, and an ethylene inhibitor

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