Higher radical scavenging activities of polyphenolic antioxidants can be ascribed to chemical reactions following their oxidation

Hotta, Hiroki; Nagano, Satomi; Ueda, Masashi; Tsujino, Yoshio; Koyama, Junko; Osakai, Toshiyuki

doi:10.1016/s0304-4165(02)00285-4

Cited by 264 publications

(233 citation statements)

References 50 publications

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“…Accordingly, its inhibitory effect on fMLP-LBCL can also involve specific inhibition of signal transduction in human PMNs. The polyphenols studied in our experiments exhibit antioxidant properties (deGraft-Johnson et al, 2007;Goupy et al, 2003;Hotta et al, 2002) and can react in vitro with ROS involved in fMLP-LBCL (Nakano et al, 2012). Thus, a direct unspecific antioxidant effect could contribute to fMLP-LBCL suppression in blood specimens preincubated with phenolics.…”

Section: Effect Of Polyphenols On Fmlp-induced Luminol Enhanced Wholementioning

confidence: 83%

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

Nowak

Zasowska-Nowak

Białasiewicz

et al. 2015

Pharmaceutical Biology

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Context: Polymorphonuclear leukocytes (PMNs) produce oxidants, contributing to systemic oxidative stress. Diets rich in plant polyphenols seem to decrease the risk of oxidative stressinduced disorders including cardiovascular disease. Objective: The objective of this study was to examine the in vitro effect of each of the 14 polyphenols on PMNs chemotaxis, intracellular calcium response, oxidants production. Materials and methods: Blood samples and PMNs suspensions were obtained from 60 healthy non-smoking donors and incubated with a selected polyphenol (0.5-10 mM) or a control solvent. We assessed resting and fMLP-dependent changes of intracellular calcium concentration ([Ca 2+ ] i ) in PMNs with the Fura-2AM method and measured fMLP-induced luminol enhanced whole blood chemiluminescence (fMLP-LBCL). Polyphenol chemoattractant activity for PMNs was tested with Boyden chambers. Results: Polyphenols had no effect on resting [Ca 2+ ] i . Unaffected by other compounds, fMLPdependent increase of [Ca 2+ ] i was inhibited by quercetin and catechol (5 mM) by 32 ± 14 and 12 ± 10% (p50.04), respectively. Seven of the 14 tested substances (5 mM) influenced fMLP-LBCL by decreasing it. Catechol, quercetin, and gallic acid acted most potently reducing fMLP-LBCL by 49 ± 5, 42 ± 15, and 28 ± 18% (p50.05), respectively. 3,4-Dihydroxyhydrocinnamic, 3,4-dihydroxyphenylacetic, 4-hydroxybenzoic acid, and catechin (5 mM) revealed distinct (p50.02) chemoattractant activity with a chemotactic index of 1.9 ± 0.8, 1.8 ± 0.7, 1.6 ± 0.6, 1.4 ± 0.2, respectively. Conclusion and discussion: Catechol, quercetin, and gallic acid at concentrations commensurate in human plasma strongly suppressed the oxidative response of PMNs. Regarding quercetin and catechol, this could result from an inhibition of [Ca 2+ ] i response.

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Section: Effect Of Polyphenols On Fmlp-induced Luminol Enhanced Wholementioning

confidence: 83%

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

Nowak

Zasowska-Nowak

Białasiewicz

et al. 2015

Pharmaceutical Biology

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“…Generally most of the amplitude of the slower phase can be attributed to secondary modifications of partially oxidized polyphenols and formation in the test tube of compounds with antioxidant properties. 32 It is known that oxidative cross-coupling that leads to the formation of dimers some, of which possess radical scavenging activity themselves, can contribute significantly to the overall AOP. 6,33 However, even for an early stage, the contribution to the initial amplitude of antioxidants formed from partially oxidized polyphenols cannot be neglected, especially for the solvents that increase the reaction rates.…”

Section: Aop Of Selected Drinks and Food Supplements Obtained By Dmentioning

confidence: 99%

The Methodology Applied in DPPH, ABTS and Folin-Ciocalteau Assays Has a Large Influence on the Determined Antioxidant Potential

Abramovič¹,

Grobin²,

Ulrih³

et al. 2017

ACSi

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Antioxidant potential (AOP) is not only the property of the matrix analyzed but also depends greatly on the methodology used. The chromogenic radicals 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (and Folin-Ciocalteu (FC) assay were applied to estimate how the method and the composition of the assay solvent influence the AOP determined for coffee, tea, beer, apple juice and dietary supplements. Large differences between the AOP values depending on the reaction medium were observed, with the highest AOP determined mostly in the FC assay. In reactions with chromogenic radicals several fold higher values of AOP were obtained in buffer pH 7.4 than in water or methanol. The type of assay and solvent composition have similar influences on the reactivity of a particular antioxidant, either pure or as part of a complex matrix. The reaction kinetics of radicals with antioxidants in samples reveals that AOP depends strongly on incubation time, yet differently for each sample analyzed and the assay applied.

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“…Among the several small molecules retained in plants, polyphenols (hydrolysabletannins/phenylpropanoids) are the most abundant antioxidants in our diet and have been shown to play a possible role in the prevention several chronic diseases (Bravo, 1998;Hotta et al, 2002;Gosslau et al, 2011b). Polyphenol antioxidants, such as resveratrol, inhibit the occurrence and/or growth of mammalian tumors (Jang et al, 2008), and a wide variety of phenolic substances derived from plants have been shown to display marked antioxidant and anti-infl ammatory activities, gallic acid and volatiles (1-butanol, dodecyl acrylate and cyclododecane), displayed a broad range of anti-oxidant, antimicrobial and anti-allergic effects Joo et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effects of Hexane Fraction from White Rose Flower Extracts via Inhibition of Inflammatory Repertoires

Lee¹,

Kim²,

Kim³

et al. 2011

Biomolecules and Therapeutics

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In this study, we determined the anti-infl ammatory activity and mechanism of action of a hexane fraction (hWRF) obtained from white Rosa hybrida fl owers by employing various assays such as quantitative real-time PCR, Western blotting, and Electrophoretic-Mobility Shift Assay (EMSA). The results revealed that the hWRF had excellent anti-infl ammatory potency by reducing infl ammatory repertoires, such as inducible nitric oxide synthase (iNOS), interleukin-1β, and cyclooxygenase-2 (COX-2) in RAW264.7 cells when stimulated with lipopolysaccharide (LPS), a pro-infl ammatory mediator. The reduction of nitric oxide (NO) release from RAW 264.7 cells supported the anti-infl ammatory effect of hWRF. Interestingly, hWRF effectively inhibited LPS-mediated nuclear factor-κB (NF-κB) p65 subunit translocation into the nucleus and extracellular signal-regulated kinase (ERK)1/2 phosphorylation, suggesting that hWRF anti-infl ammatory activity may be based on inhibition of the NF-κB and MAPK pathways. Based on the fi ndings described in this study, hWRF holds promise for use as a potential anti-infl ammatory agent for either therapeutic or functional adjuvant purposes. Abstract which contribute to their chemopreventive potential (Surh et al., 2001). Moreover, natural anti-infl ammatory or immunomodulatory plant metabolites, which are abundant in fractionated extracts, have therapeutic effects against various chronic diseases, by inducing or suppressing specifi c cellular infl ammatory activities and the associated molecular signaling pathways (Aravindaram and Yang, 2010). Some polyphenols, such as catechines from green tea, appear to be the primary bioactive compounds in the suppression of key cytokines that are secreted during the infl ammation cascade (De Mjia et al., 2009;Gosslau et al., 2011a).The rose (genus Rosa), allied to its use as a source of aromatic oils for the perfume industry, has been an alternative model for pharmaceutical candidates to treat diabetes mellitus, pain, and infl ammatory diseases (Cho et al., 2003;Ng et al., 2005). Previously, we reported that the hexane fraction from white rose fl owers, which may contain high amounts of

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Higher radical scavenging activities of polyphenolic antioxidants can be ascribed to chemical reactions following their oxidation

Cited by 264 publications

References 50 publications

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

Inhibitory effect of plant phenolics on fMLP-induced intracellular calcium rise and chemiluminescence of human polymorphonuclear leukocytes and their chemotactic activityin vitro

The Methodology Applied in DPPH, ABTS and Folin-Ciocalteau Assays Has a Large Influence on the Determined Antioxidant Potential

Anti-Inflammatory Effects of Hexane Fraction from White Rose Flower Extracts via Inhibition of Inflammatory Repertoires

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