Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity

Quémener, Maxence; Dayras, Marie; Frotté, N.; Debaets, Stella; Meur, Christophe Le; Barbier, Georges; Edgcomb, Virginia P.; Mehiri, Mohamed; Burgaud, Gaëtan

doi:10.3390/md19080411

“…Genes coding for type I and type III polyketide synthases (PKSs), nonribosomal peptide synthetases (NRPSs), and terpene synthases (TPSs) are common in fungi, including deep-sea fungi [ 57 , 58 ], being responsible for biosynthesis of a vast diversity of metabolites [ 17 , 59 ]. From deep-sea Aspergillus spp., numerous polyketides, peptides and terpenoids have been reported [ 59 , 60 , 61 ].…”

Section: Discussionmentioning

confidence: 99%

Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62

Magot

¹

,

Soen

²

,

Buedenbender

³

et al. 2023

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Despite low temperatures, poor nutrient levels and high pressure, microorganisms thrive in deep-sea environments of polar regions. The adaptability to such extreme environments renders deep-sea microorganisms an encouraging source of novel, bioactive secondary metabolites. In this study, we isolated 77 microorganisms collected by a remotely operated vehicle from the seafloor in the Fram Strait, Arctic Ocean (depth of 2454 m). Thirty-two bacteria and six fungal strains that represented the phylogenetic diversity of the isolates were cultured using an One-Strain-Many-Compounds (OSMAC) approach. The crude EtOAc extracts were tested for antimicrobial and anticancer activities. While antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA) and Enterococcus faecium was common for many isolates, only two bacteria displayed anticancer activity, and two fungi inhibited the pathogenic yeast Candida albicans. Due to bioactivity against C. albicans and rich chemical diversity based on molecular network-based untargeted metabolomics, Aspergillus versicolor PS108-62 was selected for an in-depth chemical investigation. A chemical work-up of the SPE-fractions of its dichloromethane subextract led to the isolation of a new PKS-NRPS hybrid macrolactone, versicolide A (1), a new quinazoline (−)-isoversicomide A (3), as well as three known compounds, burnettramic acid A (2), cyclopenol (4) and cyclopenin (5). Their structures were elucidated by a combination of HRMS, NMR, [α]D, FT-IR spectroscopy and computational approaches. Due to the low amounts obtained, only compounds 2 and 4 could be tested for bioactivity, with 2 inhibiting the growth of C. albicans (IC50 7.2 µg/mL). These findings highlight, on the one hand, the vast potential of the genus Aspergillus to produce novel chemistry, particularly from underexplored ecological niches such as the Arctic deep sea, and on the other, the importance of untargeted metabolomics for selection of marine extracts for downstream chemical investigations.

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“…Laser nephelometry (BMC Labtech) has already been used as an M/HTC approach. Laser nephelometry measures light scattered by particles (unicellular and/or filamentous cells) in 96 to 384‐wells and was recently used to generate >150 isolates from lower oceanic crust samples (Quemener et al., 2021). Mini/micro‐satellite primed‐PCR amplification can be used as a strategy to select unique fungal isolates from such a large collection generated by the M/HTC approach (Rédou et al., 2015).…”

Section: Methods For Studying Planktonic Marine Fungimentioning

confidence: 99%

Planktonic Marine Fungi: A Review

Peng,

Amend,

Baltar

et al. 2024

JGR Biogeosciences

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4

0

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Fungi in marine ecosystems play crucial roles as saprotrophs, parasites, and pathogens. The definition of marine fungi has evolved over the past century. Currently, “marine fungi” are defined as any fungi recovered repeatedly from marine habitats that are able to grow and/or sporulate in marine environments, form symbiotic relationships with other marine organisms, adapt and evolve at the genetic level, or are active metabolically in marine environments. While there are a number of recent reviews synthesizing our knowledge derived from over a century of research on marine fungi, this review article focuses on the state of knowledge on planktonic marine fungi from the coastal and open ocean, defined as fungi that are in suspension or attached to particles, substrates or in association with hosts in the pelagic zone of the ocean, and their roles in remineralization of organic matter and major biogeochemical cycles. This review differs from previous ones by focusing on biogeochemical impacts of planktonic marine fungi and methodological considerations for investigating their diversity and ecological functions. Importantly, we point out gaps in our knowledge and the potential methodological biases that might have contributed to these gaps. Finally, we highlight recommendations that will facilitate future studies of marine fungi. This article first provides a brief overview of the diversity of planktonic marine fungi, followed by a discussion of the biogeochemical impacts of planktonic marine fungi, and a wide range of methods that can be used to study marine fungi.

show abstract

“…Laser nephelometry (BMC Labtech) has already been used as a M/HTC approach. Laser nephelometry measures light scattered by particles (unicellular and/or filamentous cells) in 96 to 384-wells and was recently used to generate >150 isolates from lower oceanic crust samples (Quemener et al, 2021). Mini/micro-satellite primed-PCR amplification can be used as a strategy to select unique fungal isolates from such a large collection generated by the M/HTC approach (Rédou et al, 2015).…”

Section: Cultivation-based Methodsmentioning

confidence: 99%

Planktonic marine fungi: A review

Peng,

Amend,

Baltar

et al. 2023

Preprint

0

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Fungi in marine ecosystems play crucial roles as saprotrophs, parasites, and pathogens. The definition of marine fungi has evolved over the past century. Currently, “marine fungi” are defined as any fungi recovered repeatedly from marine habitats that are able to grow and/or sporulate in marine environments, form symbiotic relationships with other marine organisms, adapt and evolve at the genetic level, or are active metabolically in marine environments. While there are a number of recent reviews synthesizing our knowledge derived from over a century of research on marine fungi, this review article focuses on the state of knowledge on planktonic marine fungi from the coastal and open ocean, defined as fungi that are in suspension or attached to particles, substrates or in association with hosts in the pelagic zone of the ocean, and their roles in remineralization of organic matter and major biogeochemical cycles. This review differs from previous ones by focusing on biogeochemical impacts of planktonic marine fungi and methodological considerations for investigating their diversity and ecological functions. Importantly, we point out gaps in our knowledge and the potential methodological biases that might have contributed to these gaps. Finally, we highlight recommendations that will facilitate future studies of marine fungi. This article first provides a brief overview of the diversity of planktonic marine fungi, followed by a discussion of the biogeochemical impacts of planktonic marine fungi, and a wide range of methods that can be used to study marine fungi.

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Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity

Cited by 8 publications

References 52 publications

Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62

Bioactivity and Metabolome Mining of Deep-Sea Sediment-Derived Microorganisms Reveal New Hybrid PKS-NRPS Macrolactone from Aspergillus versicolor PS108-62

Planktonic Marine Fungi: A Review

Planktonic marine fungi: A review

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