Highly active enzymes immobilized in large pore colloidal mesoporous silica nanoparticles

Gößl, Dorothée; Singer, Helena; Chiu, Hsin-Yi; Schmidt, Alexandra M.; Lichtnecker, Martina; Engelke, Hanna; Bein, Thomas

doi:10.1039/c8nj04585b

Cited by 44 publications

(19 citation statements)

References 52 publications

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“…Carbonaceous materials such as carbon nanotubes, nanofibers, graphene, and more complex materials, such as nanoflowers have also been used for supporting enzymes since these non‐porous structures present large surface areas, low mass transfer resistance, and excellent chemical, thermal and mechanical properties . Mesoporous structures, such as mesoporous silica present high surface area and the possibility of enzyme entrapment inside the pores improving the stability and providing highly robust catalysts …”

Section: The Emergence Of Nanomaterials As Support For Enzyme Immobilmentioning

confidence: 99%

Enzyme Immobilization in Covalent Organic Frameworks: Strategies and Applications in Biocatalysis

et al. 2020

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The development of efficient catalytic systems is a fundamental aspect for the straightforward production of chemicals. During the last years, covalent organic frameworks (COFs) emerged as an exciting class of organic nanoporous materials. Due to their pre‐designable structure, they can be prepared with distinct physicochemical characteristics, specific pore sizes, and tunable functional groups. Moreover, associated with their stability in different media, these materials are considered promising supports for enzyme immobilization. Herein, it is highlighted the recent literature of enzyme immobilization in COFs, the main immobilization strategies, and the catalytic applications of these composites.

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Section: The Emergence Of Nanomaterials As Support For Enzyme Immobilmentioning

confidence: 99%

Enzyme Immobilization in Covalent Organic Frameworks: Strategies and Applications in Biocatalysis

et al. 2020

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“…This work presents a new idea to build up the spatially structured system based on active biomolecules/mesoporous silica nanoparticles, which could perform as a versatile material for numerous applications. [ 27 ] Caldas et al reported a sol–gel method to prepare mesoporous carbon ceramic materials composed of silica and graphite with different pore sizes from 7 to 21 nm, in which the glucose oxidase was immobilized as the probe enzyme. [ 28 ] The results revealed that the mesoporous support with the largest pore size exhibited the highest enzyme loading amounts and the best electrochemical response, which is attributed to the enzyme mobility and fast diffusion of analytes.…”

Section: Enzymatic Biosensorsmentioning

confidence: 99%

“…This strategy avoided the unfavorable pore blocking and showed high catalytic activity as well as the high stability. [ 27 ]…”

Section: Enzymatic Biosensorsmentioning

confidence: 99%

“…This strategy avoided the unfavorable pore blocking and showed high catalytic activity as well as the high stability. [27] The amino-functionalized SBA-15 was fabricated by an effective adsorption-crosslinking approach. At first, the acetyl cholinesterase (AChE) and BSA were adsorbed on amino-functionalized SBA-15 and enzymes due to the electrostatic adsorption, then glutaraldehyde was added to cross-link the amino group of AChE.…”

Section: The Effect Of Surface Functional Groupsmentioning

confidence: 99%

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Mesoporous Materials–Based Electrochemical Biosensors from Enzymatic to Nonenzymatic

Yang

Qiu

Yang

et al. 2019

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Mesoporous materials have drawn more and more attention in the field of biosensors due to their high surface areas, large pore volumes, tunable pore sizes, as well as abundant frameworks. In this review, the progress on mesoporous materials–based biosensors from enzymatic to nonenzymatic are highlighted. First, recent advances on the application of mesoporous materials as supports to stabilize enzymes in enzymatic biosensing technology are summarized. Special emphasis is placed on the effect of pore size, pore structure, and surface functional groups of the support on the immobilization efficiency of enzymes and the biosensing performance. Then, the development of a nonenzymatic strategy that uses the intrinsic property of mesoporous materials (carbon, silica, metals, and composites) to mimic the behavior of enzymes for electrochemical sensing of some biomolecules is discussed. Finally, the challenges and perspective on the future development of biosensors based on mesoporous materials are proposed.

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“…This kind of strategy has been successfully applied to the encapsulation of molecules in crystalline microporous hosts, producing hybrid devices now actually exploited in solar energy harvesting [20,21,22,23,24] and theranostics [25,26,27,28]. On the other hand, the encapsulation of a macromolecular system, such as a protein, requires larger pores size, hence the need of mesoporous silica matrices [14,29]. To this aim, it is particularly convenient to use mesoporous silicas like MCM-41 [30] and SBA-15 [31], which have hexagonal arrays of non-intersecting one dimensional channels and tunable nanosized pore openings.…”

Section: Introductionmentioning

confidence: 99%

Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels

Giussani

Tabacchi

Coluccia

et al. 2019

IJMS

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Incorporation of biological systems in water nanodroplets has recently emerged as a new frontier to investigate structural changes of biomolecules, with perspective applications in ultra-fast drug delivery. We report on the molecular dynamics of the digestive protein Pepsin subjected to a double confinement. The double confinement stemmed from embedding the protein inside a water nanodroplet, which in turn was caged in a nanochannel mimicking the mesoporous silica SBA-15. The nano-bio-droplet, whose size fits with the pore diameter, behaved differently depending on the protonation state of the pore surface silanols. Neutral channel sections allowed for the droplet to flow, while deprotonated sections acted as anchoring piers for the droplet. Inside the droplet, the protein, not directly bonded to the surface, showed a behavior similar to that reported for bulk water solutions, indicating that double confinement should not alter its catalytic activity. Our results suggest that nanobiodroplets, recently fabricated in volatile environments, can be encapsulated and stored in mesoporous silicas.

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Highly active enzymes immobilized in large pore colloidal mesoporous silica nanoparticles

Abstract: Carbonic anhydrase and horseradish peroxidase are immobilized inside the ordered material by click reactions. Colorimetric assays prove their catalytic activity.

Cited by 44 publications

References 52 publications

Enzyme Immobilization in Covalent Organic Frameworks: Strategies and Applications in Biocatalysis

Enzyme Immobilization in Covalent Organic Frameworks: Strategies and Applications in Biocatalysis

Mesoporous Materials–Based Electrochemical Biosensors from Enzymatic to Nonenzymatic

Confining a Protein-Containing Water Nanodroplet inside Silica Nanochannels

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