Highly Amino Acid Selective Hydrolysis of Myoglobin at Aspartate Residues as Promoted by Zirconium(IV)‐Substituted Polyoxometalates

Ly, Hong Giang T.; Absillis, Gregory; Janssens, Rik; Proost, Paul; Parac-Vogt, Tatjana

doi:10.1002/anie.201502006

Cited by 101 publications

(158 citation statements)

References 22 publications

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“…This approach relies on coordination properties of given metal ions alone (aqua ions) or in coordinatively unsaturated complexes leading to specific interactions with individual amino acid side chains or short sequences. At pH around 5, Asp-Xaa peptide bonds are selectively cleaved by metal ions as was shown for the HEWL and myoglobin hydrolysis by oxomolybdate and Zr(IV)-substituted POM [78,79]. At a lower pH 2.5, Pd(II) and Pt(II) compounds are effective hydrolytic agents specifically toward the second peptide bond upstream or the first bond downstream from the Met or His residue, respectively [47,83,84].…”

Section: Discussionmentioning

confidence: 84%

“…A similar selectivity was not observed for Glu residues. Despite the presence of a carboxyl group capable to bind oxophilic Zr(IV), Glu forms a six-membered intermediate, less stable than the five-membered one with Asp [79].…”

Section: Oxoanions and Pomsmentioning

confidence: 99%

“…Their hydrolytic activity was examined on various proteins using different POM types described in Section 2.3.1 [32,79,80]. The Zr(IV)-substituted Wells-Dawson type POM (1:2 Zr(IV)-WD POM) seems to be the most promising compound from this group so far, but the possibility of introducing a nearly infinite number of modifications into POMs gives a reasonable hope for obtaining even better artificial proteases.…”

Section: Practical Applications Of Metal-assisted Peptide Bond Hydrolmentioning

confidence: 99%

See 2 more Smart Citations

Metal assisted peptide bond hydrolysis: Chemistry, biotechnology and toxicological implications

Wezynfeld

Frączyk

Bal

2016

Coordination Chemistry Reviews

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Section: Discussionmentioning

confidence: 84%

Section: Oxoanions and Pomsmentioning

confidence: 99%

Section: Practical Applications Of Metal-assisted Peptide Bond Hydrolmentioning

confidence: 99%

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Metal assisted peptide bond hydrolysis: Chemistry, biotechnology and toxicological implications

Wezynfeld

Frączyk

Bal

2016

Coordination Chemistry Reviews

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“…Ce IV and Zr IV substituted POMs were shown to promote selective hydrolysis of a variety of water soluble proteins, ranging from flexible polypeptide such as oxidized insulin chain B (Sap et al, 2015), to larger, globular proteins such as hen egg white lysozyme (Stroobants et al, 2013, 2014d), human serum albumin (HSA) (Goovaerts et al, 2013; Stroobants et al, 2014a,b), horse heart cytochrome c (Cyt c) (Sap et al, 2016) and horse heart myoglobin (Ly et al, 2015a; Ly and Parac-Vogt, 2017). However, the potential of these POMs to act as artificial proteases toward hydrolysis of proteins in the presence of surfactants is still largely unexplored.…”

Section: Introductionmentioning

confidence: 99%

Selectivity and Reactivity of ZrIV and CeIV Substituted Keggin Type Polyoxometalates Toward Cytochrome c in Surfactant Solutions

et al. 2018

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In this paper we investigate the effect of three different types of surfactants, on the hydrolysis of Cytochrome c (Cyt c), a predominantly α helical protein containing a heme group, promoted by [Ce(α PW11O39)2]10- (CeK) and [Zr(α PW11O39)2]10- (ZrK) polyoxometalates. In the presence of SDS, Zw3 12, or CHAPS surfactants, which are commonly used for solubilizing hydrophobic proteins, the specificity of CeK or ZrK toward hydrolysis of Cyt c does not change. However, the hydrolysis rate of Cyt c by CeK was increased in the presence of SDS, but decreased in the presence of CHAPS, and was nearly inhibited in the presence of Zw3 12. The Circular dichroism and Tryptophan fluorescence spectroscopy have shown that the structural changes in Cyt c caused by surfactants are similar to those caused by POMs, hence the same specificity in the absence or presence of surfactants was observed. The results also indicate that for Cyt c hydrolysis to occur, large unfolding of the protein is needed in order to accommodate the POMs. While SDS readily unfolds Cyt c, the protein remains largely folded in the presence of CHAPS and Zw3 12. Addition of POMs to Cyt c solutions in CHAPS results in unfolding of the structure allowing the interaction with POMs to occur and results in protein hydrolysis. Zw3 12, however, locks Cyt c in a conformation that resists unfolding upon addition of POM, and therefore results in nearly complete inhibition of protein hydrolysis.

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“…Theselective cleavage of proteins and peptides is very useful in protein engineering.A lthough some artificial peptidases (such as uranyl ions, [26] metal-substituted polyoxometalate complexes, [27] MOF-808 [28] )h ave been developed, it is still very challenging to design and synthesize controllable protein-cleaving agents due to the extreme stability of the peptide bond. Theselective cleavage of proteins and peptides is very useful in protein engineering.A lthough some artificial peptidases (such as uranyl ions, [26] metal-substituted polyoxometalate complexes, [27] MOF-808 [28] )h ave been developed, it is still very challenging to design and synthesize controllable protein-cleaving agents due to the extreme stability of the peptide bond.…”

mentioning

confidence: 99%

Chiral Semiconductor Nanoparticles for Protein Catalysis and Profiling

Hao

Gao

et al. 2019

Angewandte Chemie

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In this study,v ia as imple one-step method, chiral copper sulfide quantum dots (d/l-QDs) were prepared using d-/l-penicillamine (d-/l-Pen). The anisotropyfactor of d/l-QDs was as high as 0.01. The d/l-QDs can be used as photocatalysts to cleave proteins.N otably,t he l-QDs displayed the highest catalytic performance under left-circularly polarized light irradiation. Mechanistic investigations indicate the generation of hydroxylr adicals as the reactive species that cause the cutting of proteins.Chirality,which is usually based on organic molecules, [1] has been extended to inorganic nanomaterials such as metal nanoparticles (NPs), [2] carbon NPs, [3] semiconductor NPs, [4] ZnO films, [5] metaphotonic nanostructures, [6] NP assemblies with chiral geometries, [7] and so on. [8] Chiral inorganic NPs have triggered great interest due to their easy synthesis and their tremendous applications including chiral sensing, [9] chiral separation, enantioselective catalysis,a nd advanced photonic devices. [10] Recently,c hiral metal oxides (Co 3 O 4 NPs, [11] MoO 3 NPs, [12] and WO 3Àx NPs [13] ), which exhibited intense optical activities in the visible range,h ave been synthesized using chiral ligands.S ome chiral metal sulfide nanomaterials such as HgS [14] and CdS [15] have also been prepared. These chiral NPs with deformations of the crystal lattices may be extended to other nanomaterials with tailorable chiroptical properties.Nanocrystalline copper sulfide (Cu 2Àx S) is ap -type semiconductor and am aterial of particular interest due to its promising applications in biomedicine, [16] and optoelectronic conversion (photovoltaics,p hotocatalysis,a nd thermoelectrics). [17] Endowing chirality to Cu 2Àx Sn anomaterials could enable aw ider range of potential applications for chiral Cu 2Àx Si nb iology,c hemistry,a nd physics.H owever, to date,n op ublications are available on the synthesis and application of chiral Cu 2Àx Snanomaterials.Circularly polarized light (CPL), one of the most attractive chiral sources,has been used to activate chiral assemblies to probe metal ions, [18] trigger photochemical reactions, [19] and fabricate chiral nanostructures. [20] Kotov et al. reported the use of right-(left-) handed CPL to assemble racemic CdTe

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Highly Amino Acid Selective Hydrolysis of Myoglobin at Aspartate Residues as Promoted by Zirconium(IV)‐Substituted Polyoxometalates

Cited by 101 publications

References 22 publications

Metal assisted peptide bond hydrolysis: Chemistry, biotechnology and toxicological implications

Metal assisted peptide bond hydrolysis: Chemistry, biotechnology and toxicological implications

Selectivity and Reactivity of ZrIV and CeIV Substituted Keggin Type Polyoxometalates Toward Cytochrome c in Surfactant Solutions

Chiral Semiconductor Nanoparticles for Protein Catalysis and Profiling

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