Highly concentrated omega‐3 fatty acid ethyl esters by urea complexation and molecular distillation

Magallanes, Leisa M.; Tarditto, Lorena Viviana; Grosso, Nelson; Pramparo, M.; Gayol, María F.

doi:10.1002/jsfa.9258

Cited by 30 publications

(21 citation statements)

References 21 publications

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“…Table 4 (B) shows the PUFA composition of optimal concentrate with maximum EPA + DHA content of CPUFA and concentrate with EPA + DHA content, with a value similar to the concentrate of EPA+DHA with value of EPA + DHA similar to salmon oil (CDSO). The saturated and monounsaturated fatty acids were considerably reduced in the product optimized to maximum EPA + DHA, because these kinds of fatty acids form adducts with the crystallized urea and are retained in the aqueous fraction of the subsequent washes carried out in the process of complexation, in agreement with previously reported research [ 17 , 22 , 23 , 24 , 25 , 26 , 27 ]. In the complexation process, the DHA content of CPUFA reached higher amounts in the non-complex fraction, higher than that of EPA content, a situation previously reported in the literature, being explained on the basis of a lower tendency of DHA to form urea adducts given the difference in spatial conformational structure between these fatty acids [ 25 , 28 , 29 , 30 ].…”

Section: Resultssupporting

confidence: 91%

EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet

et al. 2020

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Dietary intake of eicosapentaenoic/docosahexaenoic acid (EPA/DHA) reduces insulin resistance and hepatic manifestations through the regulation of metabolism in the liver. Obese mice present insulin resistance and lipid accumulation in intracellular lipid droplets (LDs). LD-associated proteins perilipin (Plin) have an essential role in both adipogenesis and lipolysis; Plin5 regulates lipolysis and thus contributes to fat oxidation. The purpose of this study was to compare the effects of deodorized refined salmon oil (DSO) and its polyunsaturated fatty acids concentrate (CPUFA) containing EPA and DHA, obtained by complexing with urea, on obesity-induced metabolic alteration. CPUFA maximum content was determined using the Box–Behnken experimental design based on Surface Response Methodology. The optimized CPUFA was administered to high-fat diet (HFD)-fed mice (200 mg/kg/day of EPA + DHA) for 8 weeks. No significant differences (p > 0.05) in cholesterol, glycemia, LDs or transaminase content were found. Fasting insulin and hepatic Plin5 protein level increased in the group supplemented with the EPA + DHA optimized product (38.35 g/100 g total fatty acids) compared to obese mice without fish oil supplementation. The results suggest that processing salmon oil by urea concentration can generate an EPA+DHA dose useful to prevent the increase of fasting insulin and the decrease of Plin5 in the liver of insulin-resistant mice.

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Section: Resultssupporting

confidence: 91%

EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet

et al. 2020

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“…In contrast, the purity of c 9, t 11‐CLA declined from 21.34% to 7.14% (Figure ). A similar result was found in the extraction of omega‐3 FA from the raya liver oil by urea complexation (Magallanes, Tarditto, Grosso, Pramparo, & Gayol, ). Considering the purity and yield of c 9, t 11‐CLA, we selected 1:3:9 (v/w/v) as the mass ratio of FFA, urea, and ethanol.…”

Section: Resultssupporting

confidence: 80%

The dietary c 9, t 11‐conjugated linoleic acid enriched from butter reduces breast cancer progression in vivo

et al. 2020

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The c9,t11‐conjugated linoleic acid (CLA), which is the minor polyunsaturated fatty acid (PUFA) naturally present in butter, has gained attention due to its important preventive effect against breast cancer in vitro. In this paper, the enrichment of c9,t11‐CLA from butter was optimized and the preventive effect of dietary c9,t11‐CLA against breast cancer in vivo was investigated. Results showed that the concentration of c9,t11‐CLA increased more than 10 times via a one‐step urea complexation. Furthermore, the dietary c9,t11‐CLA showed obvious preventive effect against breast cancer in decreasing the tumor weight and volume, and reducing the tumor incidence up to 50%. In addition, the expression of progesterone receptor and Ki‐67 decreased significantly with the treatment of c9,t11‐CLA. In conclusion, the dietary c9,t11‐CLA enriched from butter showed a preventive effect against breast cancer in vivo via the inhibition of the hormonal receptor and cell proliferation. Practical applications This paper provided new insight into the preparation of specific c9,t11‐CLA isomer. It can be enriched from butter in large‐scale with low‐cost by urea complexation. Meanwhile, the enriched dietary c9,t11‐CLA can be further processed into cancer prevention functional foods.

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“…A study on extraction and purification of DHA concluded that urea complexation with the correct parameters increased the DHA purity from 30% to 60% [383]. Also, urea complexation can be used in combination with molecular distillation, obtaining highly concentrated PUFAs [385]. In this method, lipids are separated based on their molecular weight.…”

Section: Concentration Of Omega-3 Fatty Acidsmentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.

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Highly concentrated omega‐3 fatty acid ethyl esters by urea complexation and molecular distillation

Abstract: The use of UC and MD together has revealed a significant improvement in the total concentration of omega-3 fatty acid ethyl esters in the final product and good application prospects. © 2018 Society of Chemical Industry.

Cited by 30 publications

References 21 publications

EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet

EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet

The dietary c 9, t 11‐conjugated linoleic acid enriched from butter reduces breast cancer progression in vivo

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

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