Highly Conserved DNA Sequence Present in Small Plasmids from Selenomonas ruminantium

Fliegerová, K.; Pažoutová, Sylvie; Pristaš, Peter; Flint, Harry J.

doi:10.1006/plas.2000.1464

Cited by 9 publications

(3 citation statements)

References 19 publications

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“…It was noticed that a certain degree of specificity exists between the SRSR type and replication protein. Consistent with this authors proposed that SRSRs might represent plasmid single‐strand origin (Nakamura et al ., ; Fliegerova et al ., ). This suggestion was questioned by the fact that some S. ruminantium plasmids (e.g.…”

Section: Discussionmentioning

confidence: 97%

“…This suggestion was questioned by the fact that some S. ruminantium plasmids (e.g. pJDB21 plasmid (Zhang & Brooker, 1993) were able to replicate independently in E. coli, where no such sequences were found (Fliegerova et al, 2000). However, this observation is not sufficient to rule out that the SRSRs might act as singlestrand origin.…”

Section: Discussionmentioning

confidence: 99%

“…Since then plasmids ranging in size from 2.5 to more than 12 kb have been reported, and up to now, at least ten small cryptic plasmids were characterized from this species (Fliegerova et al, 2000). All of the plasmids sequenced and characterized appear to be cryptic, in size not larger than 5 kb, their replication proteins with two exceptions belong to the RepL family of replication proteins and they replicate by the asymmetric rolling circle replication (RCR) mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Variability of putativerepgene cassettes inSelenomonas ruminantiumplasmids

Fecskeová

Ivan

Javorský

et al. 2012

FEMS Microbiol Lett

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Characteristic feature of the most of Selenomonas ruminantium cryptic plasmids is the presence of short, conserved sequences encompassing the gene for replication protein creating a potential rep gene cassette. PCR-based experiment was designed to analyse the genetic organization of putative plasmid rep modules and to assess S. ruminantium plasmid biodiversity. Analysed PCR amplicons contained single open reading frames encoding for putative replication proteins. While most of the derived protein sequences were often found to be conserved among putative plasmid molecules, at noncoding regions, genetic variability was observed to various extents. Complete nucleotide sequence of a plasmid was determined that contained probably a new rep gene only distantly related to known selenomonas Rep proteins but at noncoding regions shared high homology with already known plasmids. Our results document considerable structural instability and sequence variability of analysed rep gene cassettes and suggest a modular structure of S. ruminantium plasmids potentially accessible for rep gene module exchanges.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Variability of putativerepgene cassettes inSelenomonas ruminantiumplasmids

Fecskeová

Ivan

Javorský

et al. 2012

FEMS Microbiol Lett

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Plasmids ofSelenomonas ruminantium and development of host-vector system

et al. 2001

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A high frequency of plasmids was detected in the rumen bacterium Selenomonas ruminantium. Plasmids 0.9-20 kb in size were detected in more than 50% tested strains. Densitometric analysis indicated that plasmid DNA could represents more than 25% of total cellular DNA. Up to six plasmids were detected in strain S. ruminantium 18. Two smallest cryptic plasmids pSRD181 and pSRD182 from this strain were cloned into Escherichia coli vector pBluescriptSK+ and partially characterized. The plasmid pSRD181 is 1.4 kb and pSRD182 is 2.0 kb. While computer analysis of pSRD181 sequence data showed high homology with replication protein of Staphylococcus aureus plasmids, the pSRD182 sequence showed no significant homology in GenBank data. Strain S. ruminantium 28 was successfully transformed with pJW1 derived plasmid pJ1B1 using ampicillin resistance gene as marker. This is the first report on transformation of selenomonads with foreign DNA.

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Spreading and mutability ofSelenomonas ruminantium plasmids

et al. 2006

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Two small plasmids from Selenomonas ruminantium strain 19D were cloned in Escherichia coli and completely characterized. Sequence comparison indicated that the plasmids are similar to those reported in genetically vaguely related S. ruminantium strain S20. Small 1.4-kb plasmids pSRD191 and pONE430 are only distantly related (approximately 30 % for deduced Rep protein amino acid sequence) but possess a short highly conserved region outside rep gene. Larger plasmids pSRD192 and pONE429 possess large identical DNA regions in an otherwise dissimilar background. Recombination is proposed as an important mechanism of evolution and spreading of S. ruminantium plasmids.

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Highly Conserved DNA Sequence Present in Small Plasmids from Selenomonas ruminantium

Cited by 9 publications

References 19 publications

Variability of putativerepgene cassettes inSelenomonas ruminantiumplasmids

Variability of putativerepgene cassettes inSelenomonas ruminantiumplasmids

Plasmids ofSelenomonas ruminantium and development of host-vector system

Spreading and mutability ofSelenomonas ruminantium plasmids

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