Highly Controlled Heterologous Gene Expression through Combined Utilization of the Tetracycline-Repressible Transactivator and thelacRepressor

Hoshikawa, Yutaka; Amimoto, Keiko; Mizuguchi, Rumiko; Hatakeyama, Masanori

doi:10.1006/abio.1998.2742

Cited by 13 publications

(24 citation statements)

References 21 publications

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“…This appears to be necessary in order to overcome the p27 checkpoint on progenitor cell cycling ± forced expression of p27 undermines the mitogenic response to cytokines (Hoshikawa et al, 1998). Downmodulation of p27 may be integral to the heightened responsiveness of progenitor cells to multiple cytokines in combination.…”

Section: P27 Provides a Checkpoint On Progenitor Cell Expansionmentioning

confidence: 99%

Cell cycle regulators and hematopoiesis

Steinman

2002

Oncogene

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Section: P27 Provides a Checkpoint On Progenitor Cell Expansionmentioning

confidence: 99%

Cell cycle regulators and hematopoiesis

Steinman

2002

Oncogene

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“…[13][14][15][16][17][18][19][20] Here, we report an inducible siRNA vector in which the H1 promoter is under the control of the Escherichia coli lac repressor. [21][22][23] We applied the lac repressor-regulated vector to direct siRNA specific for SHP-2. By stably introducing the inducible SHP-2-siRNA vector, we established human gastric epithelial cells in which SHP-2 expression is conditionally silenced by treatment of the cells with the lactose analog isopropyl-1-thio-β-D-galactopyranoside (IPTG).…”

mentioning

confidence: 99%

Conditional gene silencing utilizing the lac repressor reveals a role of SHP‐2 in cagA‐positive Helicobacter pylori pathogenicity

et al. 2004

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“…Relative luciferase activities were determined as described for A. ator. The promoter activity is negatively and positively regulated by tetracycline and IPTG, respectively (41). Each of the JAK cDNAs was cloned into pOPTET-BSD, an expression plasmid containing the TcIP promoter and the blasticidin S resistance gene (BSD) as a selection marker.…”

Section: Methodsmentioning

confidence: 99%

Conditional Activation of Janus Kinase (JAK) Confers Factor Independence upon Interleukin-3-dependent Cells

Mizuguchi

Hatakeyama

1998

Journal of Biological Chemistry

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Cytokines play crucial roles in the growth and differentiation of hematopoietic cells. They bind to specific cell membrane receptors that usually do not possess intrinsic protein-tyrosine kinase activity. Janus kinases (JAKs) are cytoplasmic protein-tyrosine kinases that physically interact with intracellular domains of the cytokine receptors and have been implicated in playing important roles in signal transduction triggered by the cytokine-cytokine receptor interaction. However, it is still uncertain whether JAK activation alone suffices to induce cell proliferation. In this work, we modified Tyk2, a member of the JAK family, by adding a membrane localization sequence and a chemical dimerizer (coumermycin)-dependent dimerization sequence. The modified Tyk2 was activated in a coumermycin-dependent manner, and the activated Tyk2 conferred cytokine independence upon interleukin-3-dependent pro-B lymphoid cells. This cytokine-independent proliferation was completely inhibited by dominant-negative Ras. These results indicate that activation of JAK through membrane-proximal dimerization is sufficient to induce cell cycle progression and that Ras is essentially involved in JAK-triggered mitogenesis.

show abstract

Highly Controlled Heterologous Gene Expression through Combined Utilization of the Tetracycline-Repressible Transactivator and thelacRepressor

Cited by 13 publications

References 21 publications

Cell cycle regulators and hematopoiesis

Cell cycle regulators and hematopoiesis

Conditional gene silencing utilizing the lac repressor reveals a role of SHP‐2 in cagA‐positive Helicobacter pylori pathogenicity

Conditional Activation of Janus Kinase (JAK) Confers Factor Independence upon Interleukin-3-dependent Cells

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