Highly crystalline bacterial cellulose production by Novacetimonas hansenii strain isolated from rotten fruit

Neelima, S.; Sreejith, Sivaramapanicker; Shajahan, Shamna; Raj, Aparna; Vidya, L.; Aparna, V. M.; Radhakrishnan, E. K.; Sudarsanakumar, C.

doi:10.1016/j.matlet.2022.133622

Cited by 15 publications

(4 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, food waste can sometimes be utilized in different ways, too. For instance, Novacetimonas hansenii strain, which was capable of producing cellulose was isolated from rotten pomegranate for BC production in a recent research performed by Neelima et al (2023). This is an example to a different type of utilization from waste where function of the waste has changed and become the source of culture instead of being production media.…”

Section: Gluconacetobacter Xylinusmentioning

confidence: 99%

Gıda atıklarının bakteriyel selüloz üretiminde kullanımı

ÖZKAYA,

DAĞBAĞLI,

UYARCAN

2024

Ege Üniversitesi Ziraat Fakültesi Dergisi

View full text Add to dashboard Cite

Cellulose is defined as a polymer that exists in the cell walls of plant tissues and is widely used in many industrial fields. However, the recent threat of deforestation has led researchers to find alternative wood sources for cellulose production. For this reason, literature studies have focused on certain types of bacteria known to be capable of producing cellulose, such as Acetobacter, Gluconobacter, Alcaligenes, etc. It is stated that cellulose of plant origin and bacterial origin have a similar structure. Bacterial cellulose possesses a big economic and commercial potential depending on the purpose and the production method and is generally used in food applications as a fat substitute, rheology modifier, immobilization material for probiotics and enzymes, stabilizer of pickering emulsions, component of food coatings and green packaging film. Recently, it has become more prominent to use food waste as production inputs, such as beet and sugar cane molasses, fruit waste, dairy industry waste, etc. So, the utilization of industrial by-products, agro-forestry, and food industry residues as carbon sources has been providing significant advantages, such as increasing yield and reducing cost. The objective of this study was to present a general look related to bacterial cellulose production in combination with the use of food waste and future trends.

show abstract

Section: Gluconacetobacter Xylinusmentioning

confidence: 99%

Gıda atıklarının bakteriyel selüloz üretiminde kullanımı

ÖZKAYA,

DAĞBAĞLI,

UYARCAN

2024

Ege Üniversitesi Ziraat Fakültesi Dergisi

View full text Add to dashboard Cite

show abstract

“…This selection was based on previous research indicating that deteriorated fruit samples are an excellent reservoir for cellulose-degrading bacteria. [28][29][30][31][32] The process of isolation of bacteria was carried out as given below: • To synthesize cellulose from decayed fruits, 1 gram of each sample from all rotten fruits was placed in a 250 ml flask, which contained 90 ml of phosphate-buffered saline (PBS) solution. This process was conducted within a laminar flow cabinet, and the mixture was shaken for 2 hours in a shaker operating at 160 to 170 rpm.…”

Section: Isolation Of Bacteriamentioning

confidence: 99%

Identification of Cellulose Producing Bacterial Strains: An Eco-friendly and Cost-effective Approach

Muhammad,

Alburae,

Salam

et al. 2024

J. Pure Appl. Microbiol.

View full text Add to dashboard Cite

Bacterial cellulose (BC) stands out as a prominent biopolymer of global importance, distinguished by its unique advantages over plant-derived cellulose. Strains such as Acetobacter xylinum, renowned for their proficient BC production, draw considerable attention in both commercial and biomedical areas. This research aimed to selectively isolate cellulose-producing bacteria with enhanced efficiency from a variety of fruit samples utilizing a cost-effective methodology. A total of 60 fruit samples were selected, and the assessment focused on 17 strains derived from rotten banana, red apple, green apple, and pineapple samples. The evaluation encompassed an examination of bacteriological traits and cellulose synthesis, with subsequent identification of strains achieved through DNA extraction and 16S rRNA PCR analysis. The experimental findings reveal cellulose-producing strains, including model A. Xylinum (KCCM 40407) obtained from the Pharmacy lab of COMSATS University Islamabad Abbottabad Campus, Pakistan, designated as number 2, serving as a control. Notably, strains isolated from deteriorated fruits (samples 1, 4, 8, 11, 12, and 15) demonstrated the capacity to produce soluble cellulose. A. Xylinum (model strain 2) was cultured under static conditions in HS media, demonstrating remarkable efficacy for cellulose sheet production. Subsequent characterization employing scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) unveiled a nano-fiber mat featuring multi-layered fibers. This eco-friendly approach has the potential for large-scale, high-quality cellulose production, applicable in biomedical and industrial fields. The research highlights an environmentally sustainable and economically viable method for cellulose production, presenting potential applicability across biomedical and industrial arenas on a significant scale.

show abstract

“…Characteristic BC peaks at 1205 cm −1 and 1161 cm −1 , attributed to symmetrical and asymmetrical stretching vibrations of C-O-C bonds, respectively, were also observed. Other BC-specific peaks were detected at 1108 cm −1 , 1055 cm −1 , and 1031 cm −1 corresponding to C-O-C 1,4-β-glycosidic linkages or skeletal vibrations, C-O-C pyranose ring skeletal vibrations, and C-O deformation, respectively [65]. Interestingly, Molina-Ramírez et al [66] observed peaks in the spectra of BC obtained in whey that were characteristic of milk fat (2921 cm −1 and 2851 cm −1 ), esters and ethers (1743 cm −1 and 1163 cm −1 , respectively), and amine and amide groups in proteins (about 3400 cm −1 , 1540 cm −1 , and 1577 cm −1 ), which were not found in this study.…”

Section: Ftir Characterization Of Bacterial Cellulosementioning

confidence: 99%

Obtaining Bacterial Cellulose through Selected Strains of Acetic Acid Bacteria in Classical and Waste Media

et al. 2023

View full text Add to dashboard Cite

Bacterial cellulose (BC) is a natural exopolysaccharide characterized by a high purity, mechanical strength, and the ability to absorb various compounds. Obtaining BC on an industrial scale is expensive, mainly due to the high cost of the culture media. Replacing classical media with waste from the agri-food industry may be a promising way to reduce the costs when obtaining BC. The aim of these studies was to compare the effectiveness of the biosynthesis of BC in classical and waste media using two strains, Komagataeibacter xylinus K2G30 and Gluconacetobacter hansenii ATCC 23769. Classical Hestrin−Schramm, YPM, GY, and GYE media were used. The waste media were banana peels, celery pomace, fish waste, hemp seed pomace, deproteinized acid whey (DAW), and non-deproteinized acid whey (NDAW). The highest efficiency of BC biosynthesis in classical media was observed in YPM and it was 5.23 g·dm−3 with K. xylinus K2G30. In the waste media, the highest yield of BC was observed in AW, when the yield of BC was 2.99 g·dm−3 with K. xylinus K2G30. In addition, it was demonstrated that the presence of proteins and lactic acid in AW influenced the efficiency when obtaining BC in the culture of K. xylinus. The results indicate that it is possible to obtain BC from industrial waste, especially from AW.

show abstract

Highly crystalline bacterial cellulose production by Novacetimonas hansenii strain isolated from rotten fruit

Cited by 15 publications

References 20 publications

Gıda atıklarının bakteriyel selüloz üretiminde kullanımı

Gıda atıklarının bakteriyel selüloz üretiminde kullanımı

Identification of Cellulose Producing Bacterial Strains: An Eco-friendly and Cost-effective Approach

Obtaining Bacterial Cellulose through Selected Strains of Acetic Acid Bacteria in Classical and Waste Media

Contact Info

Product

Resources

About