Highly differentiated fluorescence quenching of hemoglobin using a stilbazolium dye

Unraveling the binding interaction of Toluidine blue O with bovine hemoglobin – a multi spectroscopic and molecular modeling approach

“…S3, ESI †) crossed at c ¼ 0.501, which indicates the number of TBO molecules binding with BHb to be close to unity. [57][58][59] Furthermore, the observed binding site value complements the ndings of 1 : 1 binding stoichiometry discussed in the prior section. The computed binding Table 3 The binding parameters for the interaction of TBO with BHb System Eqn ( 5) Eqn ( 8) constant and number of binding site are summarized in Table 3.…”

Characteristic Fluorescence Response of (6‐Hydroxy‐2‐naphthyl)ethenyl Pyridinium Dyes with Bovine Serum Albumin

“…We found out that a meHNEP exhibited a highly differentiated response with hemoglobin . The dye and Hb interaction induced efficient fluorescence quenching with a large Stern–Volmer constant, K SV , of up to 10 5 M −1 .…”

“…Typically, it responds to a change in dipole intensity of the medium, resulting in a bathochromic shift in less polar condition and in a hypsochromic shift in highly polar condition. In our previous report, we successfully demonstrated that a HNEP dye selectively binds to hemoglobin, causing an efficient fluorescence quenching as a result of the energy or electron‐donating and ‐accepting functionalities . Similarly, hydroxystyryl‐based dyes were reported to exhibit anomalous photophysical behaviors, in which the emission intensity heavily depended on the temperature, viscosity, and so on …”

Selective enhancement of organic dye properties through encapsulation in rotaxane orientational isomers

“…Stilbazolium salts are push-pull dyes comprising a pyridinium electron acceptor and an electron donor unit bridged by a double bond. They are studied as fluorescent sensors [1][2][3] and non-linear optical materials; [4][5][6][7] moreover, depending on their substituents, they can undergo E-Z photoisomerisation around the central double bond. 8,9 On account of the charge-transfer character of their electronic transitions, 10 the photophysical properties of these cationic dyes strongly depend on their environment, and they can be affected by encapsulation within the cavity of hosts in supramolecular complexes.…”