Highly discriminatory capacity of the PowerPlex ® Y23 System for the study of isolated populations

Núñez, Carolina; Baeta, Miriam; Fernández, Miriam; Zarrabeitia, Marı́a T.; Martı́nez-Jarreta, Begoña; Pancorbo, Marian M. de

doi:10.1016/j.fsigen.2015.04.005

Cited by 21 publications

(13 citation statements)

References 16 publications

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“…Frequencies of Y-STR haplotypes [5] were calculated using Arlequin v 3.1 software [6]. The phylogenetic relationships of Y-STR haplotypes were estimated using Network v 4.6.1.3 [7].…”

Section: Discussionmentioning

confidence: 99%

Dissection of the DF27 paternal lineage

Villaescusa

Valverde

Illescas

et al. 2015

Forensic Science International: Genetics Supplement Series

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“…Frequencies of Y-STR haplotypes [5] were calculated using Arlequin v 3.1 software [6]. The phylogenetic relationships of Y-STR haplotypes were estimated using Network v 4.6.1.3 [7].…”

Section: Discussionmentioning

confidence: 99%

Dissection of the DF27 paternal lineage

Villaescusa

Valverde

Illescas

et al. 2015

Forensic Science International: Genetics Supplement Series

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“…To compare allele designation concordance of the new assay, the same set of 134 male DNA samples from Barcelona and the Basque Country previously analyzed with PPY23 in our laboratory, was examined with the novel multiplex system. These two assays have in common the six Y‐STR markers included in the new multiplex, as well as the markers used for traceability purposes (DYS385 a/b and DYS456).…”

Section: Methodsmentioning

confidence: 99%

“…Certainly, it has proved its benefits in terms of increasing haplotype diversity and discriminatory capacity in comparison with other marker sets . Recently, our group determined the usefulness of this 23 Y‐STR panel to differentiate between similar haplotypes in population isolates, such as the native population of the Basque Country . There is already a substantial amount of worldwide populations studied with the PPY23.…”

Section: Introductionmentioning

confidence: 99%

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17 to 23: A novel complementary mini Y‐STR panel to extend the Y‐STR databases from 17 to 23 markers for forensic purposes

et al. 2017

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A Y-STR multiplex system has been developed with the purpose of complementing the widely used 17 Y-STR haplotyping (AmpFlSTR Y Filer® PCR Amplification kit) routinely employed in forensic and population genetic studies. This new multiplex system includes six additional STR loci (DYS576, DYS481, DYS549, DYS533, DYS570, and DYS643) to reach the 23 Y-STR of the PowerPlex® Y23 System. In addition, this kit includes the DYS456 and DYS385 loci for traceability purposes. Male samples from 625 individuals from ten worldwide populations were genotyped, including three sample sets from populations previously published with the 17 Y-STR system to expand their current data. Validation studies demonstrated good performance of the panel set in terms of concordance, sensitivity, and stability in the presence of inhibitors and artificially degraded DNA. The results obtained for haplotype diversity and discrimination capacity with this multiplex system were considerably high, providing further evidences of the suitability of this novel Y-STR system for forensic purposes. Thus, the use of this multiplex for samples previously genotyped with 17 Y-STRs will be an efficient and low-cost alternative to complete the set of 23 Y-STRs and improve allele databases for population and forensic purposes.

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“…Y chromosome STRs are widely used in forensic genetics for lineage and male sex identification, kinship investigations and in population genetics [1].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Y chromosomal STRs in South of Portugal population with PowerPlex ® Y23

Cruz

Ribeiro

Porto

et al. 2015

Forensic Science International: Genetics Supplement Series

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Short tandem repeats (STRs) became a vital tool in forensic genetics due to its high level of polymorphism and abundance in the human genome. Y-chromosome short tandem repeats (Y-STRs) specifically, play an important role in sexual assault cases, where the female component is in greater proportion than the male component, in complex paternity testing of male children and in the process of identification in cases of missing persons or disaster victim identification, involving profiles of male relatives. The PowerPlex 1 Y23 System is a five-dye Y-STR multiplex that allows simultaneous genotyping of 23 Y-STR loci (DYS576, DYS389I, DYS448, DYS389II, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643, DYS393, DYS458, DYS385a/b, DYS456 and Y-GATA-H4). In this study, samples of 243 unrelated male individuals living in the south of Portugal were amplified in a GeneAmp 1 9700 Thermal Cycler. PCR products were separated and detected on an ABI PRISM 1 3130 xl and the electrophoresis results analyzed with GeneMapper 1 ID software v1.2. Allele frequencies and haplotype frequencies were estimated and compared with frequencies from other European population. The results show that the study of Y-STRs using PowerPlex 1 Y23 kit, provide a powerful discrimination tool for routine forensic applications.2015 Published by Elsevier Ireland Ltd.

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Highly discriminatory capacity of the PowerPlex ® Y23 System for the study of isolated populations

Cited by 21 publications

References 16 publications

Dissection of the DF27 paternal lineage

Dissection of the DF27 paternal lineage

17 to 23: A novel complementary mini Y‐STR panel to extend the Y‐STR databases from 17 to 23 markers for forensic purposes

Analysis of Y chromosomal STRs in South of Portugal population with PowerPlex ® Y23

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