Highly efficient and sensitive detection of chloramphenicol based on chemiluminescence immunoassays with functionalized Fe3O4@SiO2@Au magnetic nanoparticles

Abstract

To avoid the long time of dialysis process, a novel Strategy that Bovine serum albumin (BSA) was immobilized on Au-MNPs at first and afterward the CAP molecules were immobilized on the BSA in sequence has been applied to Chloramphenicol (CAP) detection with chemiluminescent immunoassays (CLIA) based on functionalized Fe 3 O 4 @SiO 2 @Au magnetic nanoparticles. More importantly, this designed method was successfully applied to CAP detection with the highly efficient and sensitive. With the functionalized gold magnetic nanoparticles (Au-MNPs). Samples were detected utilizing the two different chemiluminescence immunoassays, with limits of detection (LODs) of 0.07 pg mL-1 for Method I and 0.06 pg mL-1 for Method II and a 50% inhibition concentration (IC 50) value of 0.086 ng mL-1 for Method I and 0.14 ng mL-1 for Method II for CAP respectively. Therefore, such chemiluminence immunoassays method could be easily adapted for small molecule detection in a variety of foods using Au-MNPs to conventional ILESA immunoassays in CAP detection.