Highly efficient automated extraction of DNA from old and contemporary skeletal remains

Pajnič, Irena Zupanič; Dębska, Magdalena; Pogorelc, Barbara Gornjak; Mohorčič, Katja Vodopivec; Balažic, Jože; Zupanc, Tomaž; Štefanič, B.; Geršak, Ksenija

doi:10.1016/j.jflm.2015.11.001

Cited by 37 publications

(11 citation statements)

References 41 publications

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“…In terms of skeletal remains, bone powders are the traditional type of bone samples used for DNA extraction (Amory, Huel, Bilić, Loreille, & Parsons, 2012; Karija Vlahović & Kubat, 2012; Latham & Miller, 2019; Zupanič Pajnič et al, 2016). Bone pulverization is important as it maximizes the surface area for recovery of DNA from the hydroxyapatite matrix.…”

Section: Dna Extraction From Human Remains and Non‐conventional Samplesmentioning

confidence: 99%

Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

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The extraction of DNA is a fairly standard procedure routinely undertaken in biological academic and research settings. In a forensic DNA context, DNA extraction is the crucial first step toward the generation of DNA profiles used to support convictions or exonerations in the criminal justice system. However, it is often complicated by the additional challenge of yielding sufficient quantities of clean DNA that is free from environmental inhibitors, from a wide spectrum of sample types which may contain limited quantities of biological material. While improvements in methods and technology over the decades have sped up and simplified the process of DNA extraction, several issues remain to be fully addressed—such as the incomplete separation of spermic DNA from sperm‐epithelial cell mixtures, increasing demands for ever‐faster DNA results, or even on‐scene DNA processing, and processing of difficult samples such as skeletal remains or samples contaminated by Chemical, Biological, or Radiological (CBR) agents. These challenges are often compounded by high work volumes and limited quantities of starting material. As such, this review focuses on recent trends and developments in four areas of forensic DNA extraction: (a) analysis of sexual assault evidence which often represents a significant portion of a forensic DNA laboratory's casework, (b) use of portable rapid DNA instruments which has gained much traction among law enforcement in recent times, (c) DNA extraction from difficult and CBR‐contaminated samples which, albeit rare, would be essential in a DNA laboratory's toolbox in the event of an incident, and (d) bypassing DNA extraction altogether. This article is categorized under: Forensic Biology > Forensic DNA Technologies

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Section: Dna Extraction From Human Remains and Non‐conventional Samplesmentioning

confidence: 99%

Recent trends and developments in forensic DNA extraction

Chong

Thong

Syn

2020

WIREs Forensic Science

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“…3 Amplification of a 113-bp fragment on 1.5% agarose gel. Lane marker, molecular weight marker and lanes 0-13, and 4 μl of bone from different demoralization process (from experimental groups 0, 1, 2, 4, 8, 9, and 13) were used as a template might improve STR profiles in skeletal remains as described elsewhere (Bender et al 2000;Loreille et al 2007;Bilic et al 2012;Jakubowska et al 2012;Balayan et al 2015;Zupanič Pajnič et al 2016). Therefore, the demineralization process according to experimental group no.…”

Section: Discussionmentioning

confidence: 99%

Factors influencing DNA extraction from human skeletal remains: bone characteristic and total demineralization process

Booncharoen

Khacha-ananda

Kanchai

et al. 2021

Egypt J Forensic Sci

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Background The extraction of DNA from skeletal remains with good quality and quantity is often challenging for the ability to generate DNA typing. Previous studies demonstrated the DNA extraction with total demineralization from fresh teeth and bones; however, the application in old skeletal remains has been less performed. To obtain good quality and high yield of DNA amount extracted from skeletal remains, the objective of this study was focused on exploring the factors influencing the total demineralization process to obtain developing effective methods. Results The concentration of EDTA was found to significantly enhance calcium chelation from the bone while pH of EDTA solution, incubation temperature, incubation time, and volume of EDTA solution were not significant. The optimal condition of total demineralization obtained from Placket-Burmann results represented good-quality DNA and the highest concentration of extracted DNA yield. Subsequently, the STR typing in some bone specimens processed by total demineralization process prior to DNA extraction was improved. Conclusions EDTA concentration was a key influencing factor on the total demineralization process to chelate calcium from human skeletal remains. The total demineralization process in old bone specimens probably improved the STR profiles.

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“…If the strength of the fire has been increased using fire accelerants or perhaps by the combustion of some highly combustible material in the vicinity and the bodies of the deceased have been carbonized, the soft tissue may have been destroyed and the bones severely burnt and moreor-less disintegrated. In this case, genetic analysis may be possible using the lesser burned areas and/or better preserved bones or teeth, although it is important to take into consideration during the sample-gathering process for nuclear or mitochondrial DNA analysis [39][40][41], the avoidance of areas of potential contamination which could preclude successful genetic examination, e.g., areas burnt with an accelerant, as well as to specifically locate gathering areas which may potentially contain usable information that is difficult or near impossible to visually detect.…”

Section: Burnt Human Remainsmentioning

confidence: 99%

Most Common Medico-Legal Autopsy-Related Human and Nonhuman Biological Samples for DNA Analysis

Pádár¹,

Zenke²,

Kozma³

2018

Post Mortem Examination and Autopsy - Current Issues From Death to Laboratory Analysis

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The identification and individualization of biological evidences is crucial to actual criminal investigations. In spite of the differences at the national level, all the legal processes attribute particular importance to forensic DNA analysis. However, none of the qualified results from any professional laboratory can produce substantive, valuable evidence with insufficient quality of samples and/or problems with provision of a pristine and controlled environment. The methodology and efficiency of sampling are distinct in case of living persons and in medico-legal autopsy and crime scenes. This chapter is a short overview from the basic introductory information up to ongoing research, and in accordance with constraints on the chapter size, it briefly discusses the important topics of sample collection at medico-legal autopsy for DNA analysis. The content sorts the major types of samples, reviews the common methods of sampling and the potential risk of poor sampling or contamination transfer. The corpses can be more or less degraded, which in special cases (e.g., paraffin embedded tissues, drowned, burning and/or buried cadaver) allow only for analysis of highly degraded samples. The samples can be associated with tissues of a corpse (e.g., blood, soft tissues, bone, tooth, hair) and/or additional extraneous tissues and remains, which are often mixed (e.g., blood, saliva, semen, vaginal fluid, debris of fingernails) on the corpse.

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Highly efficient automated extraction of DNA from old and contemporary skeletal remains

Cited by 37 publications

References 41 publications

Recent trends and developments in forensic DNA extraction

Recent trends and developments in forensic DNA extraction

Factors influencing DNA extraction from human skeletal remains: bone characteristic and total demineralization process

Most Common Medico-Legal Autopsy-Related Human and Nonhuman Biological Samples for DNA Analysis

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