Highly Efficient Biodegradation of Ecotoxic Dehydroabietic Acid by Resting Cells of Rhodococcus rhodochrous IEGM 107

Ivanova, Kseniya M; Гришко, В. В.; Ившина, И. Б.

doi:10.1134/s0026261722100599

Cited by 4 publications

(4 citation statements)

References 38 publications

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“…Early studies on the bioconversion of various organic compounds using resting Rhodococcus cells showed the dependence of the catalytic activity of cells on the biomass optical density (Grishko et al, 2013;Nawawi et al, 2016;Ivanova et al, 2022). The data we obtained on the biotransformation of OA and GA confirmed the previously observed dependence (Fig.…”

Section: Resultssupporting

confidence: 88%

“…Actinobacteria interacting with hydrophobic compounds are known to synthesize glycolipid biosurfactants, for which the function is also to dissolve the substrate and trigger the mechanism of its transport into the cell (Ivshina et al, 1998). It was previously shown that biotransformations of terpenoids betulin and dehydroabietic acid were accompanied by cell adhesion to the surface of substrates and by the formation of an extracellular lipophilic fluid-a biosurfactant (Tarasova et al, 2017;Cheremnykh et al, 2018;Ivanova et al, 2022).…”

Section: Resultsmentioning

confidence: 99%

“…compounds is the use of resting cells-bacterial cells in the stationary phase of growth washed from nutrients and resuspended in a buffer solution (Grishko et al, 2013;Ivshina et al, 2015;Nawawi et al, 2016;Ivanova et al, 2022). The absence of growth factors allows regulating the amount of biomass and its physiological state that helps to increase the efficiency and reduce the duration of the target process.…”

Section: Experimental Articlesmentioning

confidence: 99%

“…At the same time, the use of a buffer solution as a biotransformation medium reduces the risk of bacterial contamination, limits the growth of foreign microflora, and facilitates the process of metabolite isolation. Previously, we have demonstrated successful intensification of the biotransformation processes of plant terpenoids, namely betulin and dehydroabietic acid (Grishko et al, 2013;Ivanova et al, 2022).…”

Section: Experimental Articlesmentioning

confidence: 99%

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Rhodococcus rhodochrous IEGM 1360, an Effective Biocatalyst of C3 Oxidative Transformation of Oleanane Triterpenoids

et al. 2023

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The optimal conditions for C3 oxidative biotransformation of 1.0 g/L pentacyclic triterpenoids oleanolic (OA) and glycyrrhetinic (GA) acids were determined using the resting cells of Rhodococcus rhodochrous IEGM 1360 from the Regional Specialised Collection of Alkanotrophic Microorganisms. Resting cell suspensions (OD 600 2.6, pH 8.0, and OD 600 2.2, pH 6.0) showed the highest catalytic activity against OA and GA, resulting in the formation of 61 and 100% of their 3-oxo derivatives, respectively. Using phase contrast, atomic force, and confocal laser scanning microscopy, an adaptive response of rhodococci to the effects of OA and GA was revealed. In silico , the apoptotic activity of 3-oxo-OA and antioxidant activity of 3-oxo-GA have been assumed. In vitro, a pronounced antibacterial activity of 3-oxo-OA against Micrococcus luteus , Escherichia coli , Staphylococcus aureus , and Bacillus subtilis was shown. The absence of toxic effects of the above triterpenoids and their 3-oxo derivatives on aquatic objects and plants was demonstrated in silico and in vitro, respectively. Supplementary Information The online version contains supplementary material available at 10.1134/S0026261722603360.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Experimental Articlesmentioning

confidence: 99%

Section: Experimental Articlesmentioning

confidence: 99%

See 2 more Smart Citations

Rhodococcus rhodochrous IEGM 1360, an Effective Biocatalyst of C3 Oxidative Transformation of Oleanane Triterpenoids

et al. 2023

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<i>Rhodococcus rhodochrous</i> IEGM 1360, an Efficient Biocatalyst of C3 Oxidative Transformation of Oleanane Triterpenoids

Luchnikova,

Tarasova,

Grishko

et al. 2023

Microbiology

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Abstract—The optimal conditions for C3 oxidative biotransformation of 1.0 g/L pentacyclic triterpenoids oleanolic (OA) and glycyrrhetic (GA) acids were determined using the resting cells of Rhodococcus rhodochrous IEGM 1360 from the Regional Specialised Collection of Alkanotrophic Microorganisms. Suspensions of the resting cells with OD600 2.6 in pH 8.0 buffer and OD600 2.2 in pH 6.0 buffer showed the highest catalytic activity against OA and GA, which ensured the formation of 61 and 100% of their 3-oxo derivatives, respectively. Using phase contrast, atomic force, and confocal laser scanning microscopy, an adaptive response of rhodococci to the effects of OA and GA was revealed. In silico, the apoptotic and antioxidant activities of 3-oxo-OA and 3-oxo-GA, respectively, have been assumed. In vitro, a pronounced antibacterial activity of 3-oxo-OA against Micrococcus luteus, Escherichia coli, Staphylococcus aureus, and Bacillus subtilis was shown. The absence of toxic effects of the triterpenoids and their 3-oxo derivatives on aquatic and plant objects was demonstrated in silico and in vitro, respectively.

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Bioinformatics Analysis of the Genome of Rhodococcus rhodochrous IEGM 1362, an (−)-Isopulegol Biotransformer

Maltseva,

Plotnitskaya,

Krivoruchko

et al. 2024

Genes

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A genome of Rhodococcus rhodochrous IEGM 1362 was sequenced and annotated. This strain can transform monoterpene alcohol (–)-isopulegol with the formation of two novel pharmacologically promising metabolites. Nine genes encoding cytochrome P450, presumably involved in (–)-isopulegol transformation, were found in the genome of R. rhodochrous IEGM 1362. Primers and PCR conditions for their detection were selected. The obtained data can be used for the further investigation of genes encoding enzymes involved in monoterpene biotransformation.

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Highly Efficient Biodegradation of Ecotoxic Dehydroabietic Acid by Resting Cells of Rhodococcus rhodochrous IEGM 107

Cited by 4 publications

References 38 publications

Rhodococcus rhodochrous IEGM 1360, an Effective Biocatalyst of C3 Oxidative Transformation of Oleanane Triterpenoids

Rhodococcus rhodochrous IEGM 1360, an Effective Biocatalyst of C3 Oxidative Transformation of Oleanane Triterpenoids

<i>Rhodococcus rhodochrous</i> IEGM 1360, an Efficient Biocatalyst of C3 Oxidative Transformation of Oleanane Triterpenoids

Bioinformatics Analysis of the Genome of Rhodococcus rhodochrous IEGM 1362, an (−)-Isopulegol Biotransformer

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