Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol

Benjaphokee, Suthee; Hasegawa, Daisuke; Yokota, Daiki; Asvarak, Thipa; Auesukaree, Choowong; Sugiyama, Minetaka; Kaneko, Yoshinobu; Boonchird, Chuenchit; Harashima, Satoshi

doi:10.1016/j.nbt.2011.07.002

Cited by 100 publications

(45 citation statements)

References 40 publications

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“…However, the optimal temperature for both enzymes was 60 C, much higher than the growth and fermentation temperatures for yeast. Thus, the enzymes will work more efficiently if they are expressed in S. cerevisiae strains with stress tolerance to high temperature (Benjaphokee et al, 2012). Given the effects of the metal ions tested, only Ca 2+ could enhance both enzyme activities (Tables 2 and 3).…”

Section: Discussionmentioning

confidence: 99%

Direct fermentation of amorphous cellulose to ethanol by engineered Saccharomyces cerevisiae coexpressing Trichoderma viride EG3 and BGL1

Yang¹,

Tang²,

Wang³

et al. 2014

J. Gen. Appl. Microbiol.

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Direct ethanol fermentation from amorphous cellulose was achieved using an engineered industrial Saccharomyces cerevisiae strain. Two cellulase genes endoglucanase (eg3) and β-glucosidase (bgl1) were obtained from Trichoderma viride and integrated into the genome of S. cerevisiae. These two cellulases could be constitutively coexpressed and secreted by the recombinant strain S. cerevisiae-eb. The enzyme activities were analyzed in the culture supernatants, with the highest endoglucanase activity of 2.34 units/ml and β-glucosidase activity of 0.95 units/ml. The effects of pH, temperature and metal ions on enzyme activities were analyzed. The coexpression strain S. cerevisiae-eb could grow in carboxymethyl cellulose (CMC) and utilize it as the single carbon source. The 20 g/L CMC as a model substrate of amorphous cellulose was used in fermentation. The ethanol production reached 4.63 g/L in 24 h, with the conversion ratio of 64.2% compared with the theoretical concentration. This study demonstrated that the engineered industrial strain S. cerevisiae-eb could convert amorphous cellulose to ethanol simultaneously and achieve consolidated bioprocessing (CBP) directly.

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Section: Discussionmentioning

confidence: 99%

Direct fermentation of amorphous cellulose to ethanol by engineered Saccharomyces cerevisiae coexpressing Trichoderma viride EG3 and BGL1

Yang¹,

Tang²,

Wang³

et al. 2014

J. Gen. Appl. Microbiol.

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“…Because of their dominant Htg þ phenotype, it is feasible to use these isolates in a breeding program to develop a superior hybrid yeast strain that displays multiple characteristics such as tolerance to multiple stresses and high ethanol productivity that are useful for ethanol fermentation. Toward this goal, we were recently successful in breeding a strain that was tolerant to high temperature (41 C) and high acidity (pH 3.5) and also capable of producing and tolerating high ethanol yields (28).…”

Section: Discussionmentioning

confidence: 99%

Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits

Auesukaree

Koedrith

Saenpayavai

et al. 2012

Journal of Bioscience and Bioengineering

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“…To improve ethanol yield and productivity and to search for less expensive technology for the production of ethanol, the development of a fermentation process at high temperature with a high concentration substrate is necessary (Morimura et al 1997). The advantages of fermentation at high temperature (40-45°C) are not only reduced cooling, simultaneous saccharification and fermentation (SSF) and downstream distillation costs but also minimization of the risk of contamination (Benjaphokee et al 2012;Nevoigt 2008;Olofsson et al 2008). An increase in fermentation temperature of only 5°C can greatly affect ethanol production costs (Abdel-Banat et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional analysis of Saccharomyces cerevisiae during high-temperature fermentation

et al. 2013

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DNA microarrays were used to investigate the transcriptional response of Saccharomyces cerevisiae genes under high temperature fermentation. Up to 35.73 % of yeast genes were up-regulated or down-regulated at least two-fold in their expression level at the late stage of fermentation. Nine genes involved in the pathways of glycolysis, ethanol generation and stress response were selected for study of their transcription profiles during high temperature fermentation processes by using quantitative real-time PCR assay. Our data indicated that the genes involved in trehalose biosynthesis and encoding heat shock proteins (HSPs) were significantly induced, while the genes involved in ethanol production were down-regulated during the 40°C fermentation. Specially, HSP26 displayed the highest transcription level of 166.19±15.82-fold at 6 h, indicating that this gene may play important roles at the onset of 40°C fermentation. Moreover, transcription levels of the nine genes were reduced significantly and returned to normal levels compared with controls after the samples were treated at 30°C for another 2 h. The results of this study suggest that these genes and their related pathways are involved in the response to high temperature; these findings will be helpful in improving the characteristics and fermentation capacity of industrial yeast strains by metabolic engineering.

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Highly efficient bioethanol production by a Saccharomyces cerevisiae strain with multiple stress tolerance to high temperature, acid and ethanol

Cited by 100 publications

References 40 publications

Direct fermentation of amorphous cellulose to ethanol by engineered Saccharomyces cerevisiae coexpressing Trichoderma viride EG3 and BGL1

Direct fermentation of amorphous cellulose to ethanol by engineered Saccharomyces cerevisiae coexpressing Trichoderma viride EG3 and BGL1

Characterization and gene expression profiles of thermotolerant Saccharomyces cerevisiae isolates from Thai fruits

Transcriptional analysis of Saccharomyces cerevisiae during high-temperature fermentation

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