Highly efficient cascading synergy of cancer photo-immunotherapy enabled by engineered graphene quantum dots/photosensitizer/CpG oligonucleotides hybrid nanotheranostics

Wu, Chunhui; Guan, Xiaotian; Xu, Jiming; Zhang, Yingxue; Liu, Qiuyue; Tian, Yuan; Li, Shun; Qin, Xiang; Yang, Hong; Liu, Yiyao

doi:10.1016/j.biomaterials.2019.03.020

Cited by 99 publications

(71 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fortunately, the advent of nanotechnology has led to the development of different kinds of gene delivery systems, such as mesoporous silica nanoparticles 12, nanographene 13, PLGA-based nanoparticles 14, gold nanoparticles 15, and iron oxide nanoparticles. Such delivery systems are able to protect and transfer plasmid DNA 16, 17, oligonucleotides 18 and fluorescent probes 19 into living cells. For example, Choi et al constructed nanoprobes from iron oxide nanoparticles (IONPs) and hairpin MBs to detect miR-200a in living cells 20; Yin et al utilized PEI coated magnetic core-shell nanoparticles to deliver a heat-inducible gene vector that encodes TRAIL into mesenchymal stem cells 21.…”

Section: Introductionmentioning

confidence: 99%

Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging

et al. 2019

Self Cite

View full text Add to dashboard Cite

Background & Aims: The use of antisense oligonucleotide-based nanosystems for the detection and regulation of tumor-related gene expression is thought to be a promising approach for cancer diagnostics and therapies. Herein, we report that a cubic-shaped iron oxide nanoparticle (IONC) core nanobeacon is capable of delivering an HSP90α mRNA-specific molecular beacon (HSP90-MB) into living cells and enhancing T2-weighted MR imaging in a tumor model.Methods: The nanobeacons were built with IONC, generation 4 poly(amidoamine) dendrimer (G4 PAMAM), Pluronic P123 (P123) and HSP90-MB labeled with a quencher (BHQ1) and a fluorophore (Alexa Fluor 488).Results: After internalization by malignant cells overexpressing HSP90α, the fluorescence of the nanobeacon was recovered, thus distinguishing cancer cells from normal cells. Meanwhile, MB-mRNA hybridization led to enzyme activity that degraded DNA/RNA hybrids and resulted in downregulation of HSP90α at both the mRNA and protein levels. Furthermore, the T2-weighted MR imaging ability of the nanobeacons was increased after PAMAM and P123 modification, which exhibited good biocompatibility and hemocompatibility.Conclusions: The nanobeacons show promise for applicability to tumor-related mRNA detection, regulation and multiscale imaging in the fields of cancer diagnostics and therapeutics.

show abstract

Section: Introductionmentioning

confidence: 99%

Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging

et al. 2019

Self Cite

View full text Add to dashboard Cite

show abstract

“…Reproduced with permission. [ 23 ] Copyright 2019, Elsevier Ltd. c) The structure of COOH‐PLL, the schematic illustration of cryopreserving NK cells and evaluation of cryopreservation effect of NK cells. Reproduced with permission.…”

Section: Structural Designs Of Polymeric Nonviral Gene Delivery Systemsmentioning

confidence: 99%

“…[ 22 ] Recently, Wu et al. reported a novel photo/immune‐active hybrid nanostructure, which was based on PLL cationic polymer, graphene quantum dot (GQD), and nucleic acid adjuvant oligodeoxynucleotides (ODN) containing cytosine‐phosphate‐guanine (CpG) motifs, [ 23 ] as shown in Figure 1b. Due to the photoresponsibility of GQD, this nanocomposite could release endogenous tumor antigen and promote the immune stimulation response in the tumor microenvironment for enhanced cancer growth inhibition, indicating its promising application in cancer immunotherapy.…”

Section: Structural Designs Of Polymeric Nonviral Gene Delivery Systemsmentioning

confidence: 99%

Polymeric Nonviral Gene Delivery Systems for Cancer Immunotherapy

Cai

et al. 2020

Advanced Therapeutics

View full text Add to dashboard Cite

Unlike viral vectors with their undesired safety or immunogenicity concerns, biocompatible polymeric nonviral vectors as gene delivery systems are more promising in gene or cell therapy. Evidence has demonstrated that the rational design of polymeric nonviral gene vectors with optimal structure, charge density, biocompatibility, and stimulus responsiveness can deliver therapeutic genes or gene vaccines (in terms of deoxyribonucleic acid DNA or ribonucleic acid RNA) into tumor‐associated immunocytes (i.e., macrophages, T cells, or dendritic cells) in an effective and controllable manner for enhanced cancer immunotherapy. However, a timely and systematic summary of this subject is lacking. This review presents an overview of polymeric nonviral carriers with immune cell transfection ability and their potential applications in cancer immunotherapy; it also provides a tutorial for designing polymeric immune‐cell genetic modification vector, although there are still few vectors in the product pipeline. With the rapid growth of immunotherapy in cancer treatment and knowledge accumulation in vector structure design, polymeric nonviral gene delivery systems may provide new hope for tumor therapy.

show abstract

“…In this scenario, the IR820‐hydrogel‐based hyperthermia can effectively eliminate the primary tumor and generated tumor specific‐antigens for assisted immunotherapy, while CpG immunoadjuvant could significantly promote the antigen presenting the ability of APCs. Consequently, the antigen‐specific CTLs response was further improved to elicit antitumor immunity . Researchers also developed poly(lactic‐ co ‐glycolic) acid (PLGA) NPs for codelivery of a TLR7 agonist (imiquimod), and CTLA‐4 checkpoint inhibitor, along with a photothermal agent (ICG) for combination PTT immunotherapy.…”

Section: Evidence Of Nanoparticle‐mediated Photoimmunotherapy For Indmentioning

confidence: 99%

Immunological Consequences of Nanoparticle‐Mediated Antitumor Photoimmunotherapy

Hameed

Mustafa

et al. 2019

Advanced Therapeutics

View full text Add to dashboard Cite

Conventional phototherapies have been thought to destroy the malignant cells through apoptosis or necrosis, shut down tumor microvasculature, and activate the host immune system. Accumulating evidence indicates that photothermal agents stimulate both innate and adaptive arms of host immune systems by i) priming tumor antigen-specific T-cell responses, ii) attracting lymphocytes into the tumor site, and iii) modulation of the tumor microenvironment (TME). However, the salutary effect of traditional phototherapies is hindered by its limited penetration depth in tissues. Recent advances in cancer immunotherapies have significantly transformed the landscape of cancer treatment from conventional phototherapies to most standard cancer therapy regimen. Therefore, phototherapies synergized with immunotherapy, maximize the anticancer efficacy by overcoming immunosuppressive TME and elucidating immune responses outside the irradiation area. This synergism is further strengthened by nanomaterial-based strategies to form innovative nanocarriers for efficient and simultaneous delivery of photosensitizers and immunomodulators at the desired target. These nanocarriers can improve the accumulation and retention of both photosensitizer and immunomodulators within the target location, thereby enhancing safety and efficacy. Herein, the systematic effects of phototherapies on the immune system are summarized and the ways in which nanomaterial-based photoimmunotherapy can facilitate the eradication of both primary and metastatic tumors are discussed.

show abstract

Highly efficient cascading synergy of cancer photo-immunotherapy enabled by engineered graphene quantum dots/photosensitizer/CpG oligonucleotides hybrid nanotheranostics

Cited by 99 publications

References 50 publications

Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging

Dendrimer-Functionalized Superparamagnetic Nanobeacons for Real-Time Detection and Depletion of HSP90α mRNA and MR Imaging

Polymeric Nonviral Gene Delivery Systems for Cancer Immunotherapy

Immunological Consequences of Nanoparticle‐Mediated Antitumor Photoimmunotherapy

Contact Info

Product

Resources

About